Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Date (from‐to) : 2013/04 -2019/03
Author : Maeyama Jun-ichi; Isaka Masanori
When G9.1, which is a novel oligo DNA containing CpG motif, was nasally administered as a mucosal adjuvant with diphtheria toxoid, enhancement of the specific antibody production and that of the diphtheria antitoxin titers were observed. Involvement of TLR9 and plasmacytoid dendritic cells (pDC) in adjuvanticity of G9.1 was confirmed. Coculture of mouse bone marrow cells with G9.1 strongly induced IFN-αproduction. T-bet, which is a specific transcription factor for Th1 cells, was strongly expressed. Therefore, G9.1 was shown to be a mucosal adjuvant that enhances Th1 immunity via TLR9 of pDC. And, also, IFN-α produced by pDC near the mucosal membrane is an important factor as mechanisms of adjuvanticity of G9.1.
The evaluation system of tuberculosis booster vaccine efficacy by delayed type hypersensitivity reaction against tuberculosis antigen was established by guinea pigs administered BCG with low immunostimulatory ability as prime immunization.