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ITO Saotomo

    Graduate School of Pharmaceutical Sciences Department of Molecular and Cellular Health Science　Associate Professor
Contact： s-itohphar.nagoya-cu.ac.jp
Last Updated :2024/09/07

Researcher Information

URL

J-Global ID

Research Interests

  • 免疫回避   免疫回避   黄色ブドウ球菌   

Research Areas

  • Life sciences / Pharmaceuticals - health and biochemistry

Education

  •        -   Nagoya City University

Published Papers

MISC

Industrial Property Rights

Research Grants & Projects

  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2019/04 -2022/03 
    Author : Itoh Saotomo
     
    Staphylococcus aureus is though to be associated with allergic disorders. In this study, We showed that two staphylococcal toxins, alpha-hemolysin and Staphylococcal superantigen-like 12 (SSL12) activate the important immune cells in the development of the allergic inflammation, mast cells and basophils.We also identified the responsible amino acid residues of SSL12 for mast cells activation. These results demonstrate the involvement of staphylococcal toxins in the development of allergic disorders.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2016/04 -2019/03 
    Author : Itoh Saotomo
     
    Staphylococcal superantigen like is a family of exoproteins, that consist of 14 SSLs and contributes to immune evasion of S. aureus. We attempted to identify their unknown host target molecules, and specify responsible region for the binding to the target molecules. In this study, we have identified the responsible regions for the binding of SSL10 to prothrombin, that of SSL10 binding to IgG, and that of SSL5 interfere for the proteolytic activity of MMP-9. We have revealed that SSL12 activates mast cells. SSL12 induces the degranulation of mast cells and cytokine production. SSL12 increased vascular permeability when it applied intradermally.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2013/04 -2016/03 
    Author : Itoh Saotomo
     
    Staphylococcal superantigen-like proteins(SSL) recognize and disturb various host immune molecules, and involved in immune evasion of the bacteria. In this study, we analayzed molecular mechanisms of binding SSLs to host target molecules.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2010 -2012 
    Author : ITOH Saotomo
     
    Staphylococcal superantigen-like (SSL) proteins, composed 14 member of exoprotein, share structural similarity with superantigens but no superantigenic activity. Several SSLs are known to be involved in immune evasion of S. aureus, however remaining SSLs are not identified their target proteins. In this study, we showed that SSL3 binds to TLR2, SSL8 binds to tenascin C, SSL10 binds to phosphatidyl serine and coagulation factors. We also showed the interactions between SSLs and the target proteins interferes the physiological functions of them.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2007 -2009 
    Author : ITOH Saotmo
     
    During hemodialysis, the contact of blood components with artificial hemodialysis membranes induce the activation of leukocytes and production of reactive oxygen species (ROS), followed with development of long-term dialysis related complications. In this study, I revealed the mechanism of leukocyte activation induced by hemodialysis membranes, i.e. the contact of blood with hemodialysis membrane evokes expression of P-selectin on activated platelets, formation of platelet-neutrophil microaggregates and production of ROS by neutrophils. The contact of plasma with hemodialysis membrane also activates complement system and induces formation of anaphylatoxin C5a and cap formation of P-selectin ligand on neutrophils which enhances formation of the microaggregates. I found alpha-tocopherol and glycyrrhizin could prevent the interaction of platelets and neutrophils and activation of neutrophils during hemodialysis.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2007 -2008 
    Author : TSUJI Tsutomu; ITOH Saotomo; OKU Teruaki; SAITO Yuta; MIZUNO Hiromi; SEKINE Wakana; GOTOU Toshie; SHIMADA Kentaro; KAMOSHIDA Go
     
    悪性度の高い肝がんや胃がんに高発現する細胞接着分子α3β1インテグリンのがんの浸潤・転移における役割について解析した. このインテグリンと組織マトリックスに含まれるラミニンの相互作用により細胞運動や浸潤能が高まり, またマトリックス分解酵素の産生が亢進し, これらが相乗的に作用し, がんの浸潤・転移に対し促進的に働くことが推察された. この過程を制御することによりがん転移の予防に資するものと考えられる.
  • 日本学術振興会：科学研究費助成事業 若手研究(B)
    Date (from‐to) : 2004 -2006 
    Author : 伊藤 佐生智
     
    p57は免疫細胞に特異的に発現するアクチン結合タンパク質であり,食作用をはじめとする細胞運動に関与していると考えられている.我々は白血球の貪食の際にp57がF-アクチンとともにファゴソームに集積し,ファゴソームの成熟に先行してファゴソーム膜から解離すること,p57はこの過程において一過性にリン酸化されることを見出している.一方,細胞内寄生菌である結核菌はp57のファゴソームからの解離を抑制してファゴソーム成熟を抑制し,細胞内での生存を可能にしているという報告があり,p57とアクチン細胞骨格系との相互作用の解析は細胞内寄生菌の生存機構の解明とこれを標的とした細胞内寄生菌感染症の新規治療法の開発に寄与すると考えられる.p57はN末端のWDリピート領域とC末端のロイシンジッパーを含むコイルドコイル領域からなる.WDリピート領域中には少なくとも2ヶ所アクチン結合能が存在し,一方でコイルドコイル領域は二量体形成に関与していることを明らかにしている.今年度の研究においてp57のリン酸化状態と細胞骨格系への結合の関係を検討するため,HL60細胞より調整した界面活性剤不溶性の細胞骨格成分へのp57の分布を検討したところ,PMA処理によりp57は細胞骨格から解離し,リン酸化の抑制により細胞骨格への結合が促進された.このことはGFP-p57強制発現細胞においても示された.また細胞骨格に結合しているp57のリン酸化状態を二次元電気泳動により解析した結果,細胞骨格結合性のp57は低リン酸化型であり,可溶性のp57は高リン酸化型であることが示された.我々は以前にp57が二量体形成することによりアクチンの架橋形成を媒介することを見出している.今回の結果よりp57は二量体形成とリン酸化による調節を介してファゴソームの形成と成熟におけるアクチン細胞骨格の再構成の調節を担っている可能性が示された.

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