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HAYASHI Hidetoshi

FacultyGraduate School of Pharmaceutical Sciences Department of Cell Signaling, Executives
PositionProfessor
Mailhhayashiphar.nagoya-cu.ac.jp
HomepageURL
Birthday
Last Updated :2019/11/30

Researcher Profile and Settings

Education

  •   1978 04  - 1982 03 , The University of Tokyo

Association Memberships

  • the American Society for Cell Biology
  • International Cytokine Society

Research Activities

Research Areas

  • Pharmacy, Environmental and hygienic pharmacy
  • Pharmacy, Biological pharmacy
  • Oncology, Tumor biology

Research Interests

    Epithelial-Mesenchymal Transition, drug repositioning, ER stress, cancer molecular target drugs, TGF-beta, deubiquitination, cellular stress, inflammation, glucose and lipid metabolism

Published Papers

  • Kurarinone from Sophora flavescens roots triggers ATF4 activation and cytostatic effects through PERK phosphorylation, Sakiko Nishikawa, Yuka Itoh, Muneshige Tokugawa, Yasumichi Inoue, Ken ichi Nakashima, Yuka Hori, Chiharu Miyajima, Kou Yoshida, Daisuke Morishita, Nobumichi Ohoka, Makoto Inoue, Hajime Mizukami, Toshiaki Makino, Hidetoshi Hayashi, Molecules, 24, (17) , 08 , Refereed
  • TRB1 negatively regulates gluconeogenesis by suppressing the transcriptional activity of FOXO1, Kaori Tsuzuki, Yuka Itoh, Yasumichi Inoue, Hidetoshi Hayashi, FEBS Letters, 593, (3) 369 - 380, 02 , Refereed
  • Lysine-specific demethylase 1 (LSD1/KDM1A) is a novel target gene of c-myc, Mai Nagasaka, Kaori Tsuzuki, Yu Ozeki, A. Muneshige Tokugawa, Nobumichi Ohoka, Yasumichi Inoue, Hidetoshi Hayashi, Biological and Pharmaceutical Bulletin, 42, (3) 481 - 488, 01 , Refereed
  • Anti-tumorigenic activity of chrysin from oroxylum indicum via non-genotoxic p53 activation through the ATM-Chk2 pathway, Mai Nagasaka, Ryoko Hashimoto, Yasumichi Inoue, Kan'ichiro Ishiuchi, Michiyo Matsuno, Yuka Itoh, Muneshige Tokugawa, Nobumichi Ohoka, Daisuke Morishita, Hajime Mizukami, Toshiaki Makino, Hidetoshi Hayashi, Molecules, 23, (6) , 01 , Refereed
  • The CDK inhibitor p21 is a novel target gene of ATF4 and contributes to cell survival under ER stress, Yasumichi Inoue, Shiori Kawachi, Tsubasa Ohkubo, Mai Nagasaka, Shogo Ito, Keishi Fukuura, Yuka Itoh, Nobumichi Ohoka, Daisuke Morishita, Hidetoshi Hayashi, FEBS Letters, 591, (21) 3682 - 3691, 11 , Refereed
  • Gerontoxanthone B from Maclura cochinchinensis var. gerontogea exhibits anti-inflammatory potential as an aryl hydrocarbon receptor agonist, Ken ichi Nakashima, Takumi Ogiwara, Takao Hirai, Toshiyuki Tanaka, Hiroko Murata, Kouichi Kaburagi, Yoshiaki Fujii-Kuriyama, Hidetoshi Hayashi, Makoto Inoue, Bioorganic and Medicinal Chemistry, 25, (16) 4253 - 4258, 01 , Refereed
  • Atranorin and lecanoric acid antagonize TCDD-induced xenobiotic response element-driven activity, but not xenobiotic response element-independent activity, Ken ichi Nakashima, Hiroki Tanabe, Yoshiaki Fujii-Kuriyama, Hidetoshi Hayashi, Makoto Inoue, Journal of Natural Medicines, 70, (3) 476 - 482, 07 , Refereed, © 2016, The Japanese Society of Pharmacognosy and Springer Japan. Lichens are symbiotic organisms that consist of fungi and photosynthetic symbionts (algae and/or cyanobacteria). Previous studies of their constituents
  • Regulation of epithelial-mesenchymal transition by E3 ubiquitin ligases and deubiquitinase in cancer, Yasumichi Inoue, Yuka Itoh, Koichi Sato, Fumihiro Kawasaki, Chihiro Sumita, Takahito Tanaka, Daisuke Morishita, Hidetoshi Hayashi, Current Cancer Drug Targets, 16, (2) 110 - 118, 02 , Refereed
  • Tribbles-related protein family members as regulators or substrates of the ubiquitin-proteasome system in cancer development, Satoshi Sakai, Chiharu Miyajima, Chiharu Uchida, Yuka Itoh, Hidetoshi Hayashi, Yasumichi Inoue, Current Cancer Drug Targets, 16, (2) 147 - 156, 02 , Refereed
  • Positive regulation of interleukin-2 expression by a pseudokinase, tribbles 1, in activated T cells, Chiharu Miyajima, Yuka Itoh, Yasumichi Inoue, Hidetoshi Hayashi, Biological and Pharmaceutical Bulletin, 38, (8) 1126 - 1133, 08 , Refereed
  • TGF-β decreases the stability of IL-18-induced IFN-γ mRNA through the expression of TGF-β-induced tristetraprolin in KG-1 cells, Yasumichi Inoue, Kenji Abe, Kikuo Onozaki, Hidetoshi Hayashi, Biological and Pharmaceutical Bulletin, 38, (4) 536 - 544, 04 , Refereed
  • Pseudokinase tribbles 1 (TRB1) negatively regulates tumor-suppressor activity of p53 through p53 deacetylation, Chiharu Miyajima, Yasumichi Inoue, Hidetoshi Hayashi, Biological and Pharmaceutical Bulletin, 38, (4) 618 - 624, 04 , Refereed
  • The NADPH oxidase inhibitor diphenyleneiodonium activates the human TRPA1 nociceptor, Hiroka Suzuki, Noriyuki Hatano, Yukiko Muraki, Yuka Itoh, Satoko Kimura, Hidetoshi Hayashi, Kikuo Onozaki, Yoshiaki Ohi, Akira Haji, Katsuhiko Muraki, American Journal of Physiology - Cell Physiology, 307, (4) C384 - C394, 08 , Refereed
  • A novel transgenic mouse model carrying human tribbles related protein 3 (TRB3) gene and its site specific phenotype, Yuto Sakai, Katsumi Fukamachi, Mitsuru Futakuchi, Ichiro Miyoshi, Hiroyuki Tsuda, Masumi Suzui, Hidetoshi Hayashi, Biological and Pharmaceutical Bulletin, 37, (6) 1068 - 1074, 01 , Refereed
  • Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells, Yuto Sakai, Katsumi Fukamachi, Mitsuru Futakuchi, Hidetoshi Hayashi, Masumi Suzui, Oncology Reports, 30, (1) 64 - 70, 07 , Refereed
  • Staphylococcal superantigen-like protein 8 (SSL8) binds to tenascin C and inhibits tenascin C-fibronectin interaction and cell motility of keratinocytes, Saotomo Itoh, Natsuko Yamaoka, Go Kamoshida, Takemasa Takii, Tsutomu Tsuji, Hidetoshi Hayashi, Kikuo Onozaki, Biochemical and Biophysical Research Communications, 433, (1) 127 - 132, 03 , Refereed
  • Hypoxia-inducible factor-1α (HIF1α) switches on transient receptor potential ankyrin repeat 1 (TRPA1) gene expression via a hypoxia response element-like motif to modulate cytokine release, Noriyuki Hatano, Yuka Itoh, Hiroka Suzuki, Yukiko Muraki, Hidetoshi Hayashi, Kikuo Onozaki, Ian C. Wood, David J. Beech, Katsuhiko Muraki, Journal of Biological Chemistry, 287, (38) 31962 - 31972, 09 , Refereed
  • Dihydrotestosterone inhibits interleukin-1α or tumor necrosis factor α-induced proinflammatory cytokine production via androgen receptor-dependent inhibition of nuclear factor-κB activation in rheumatoid fibroblast-like synovial cell line, Jian Xu, Yuka Itoh, Hidetoshi Hayashi, Takemasa Takii, Keiji Miyazawa, Kikuo Onozaki, Biological and Pharmaceutical Bulletin, 34, (11) 1724 - 1730, 11 , Refereed
  • Reduction of transforming growth factor-β type II receptor is caused by the enhanced ubiquitin-dependent degradation in human renal cell carcinoma, Hirotaka Fukasawa, Tatsuo Yamamoto, Yoshihide Fujigaki, Taro Misaki, Naro Ohashi, Tatsuya Takayama, Sayuri Suzuki, Soichi Mugiya, Toshiaki Oda, Chiharu Uchida, Kyoko Kitagawa, Takayuki Hattori, Hidetoshi Hayashi, Seiichiro Ozono, Masatoshi Kitagawa, Akira Hishida, International Journal of Cancer, 127, (7) 1517 - 1525, 10 , Refereed
  • Cigarette smoke condensate extracts augment collagen-induced arthritis in mice, Satomi Chujo, Shosuke Okamoto, Ryohei Sunahara, Miki Adachi, Kyohei Yamada, Hidetoshi Hayashi, Takemasa Takii, Kazuichi Hayakawa, Kikuo Onozaki, International Immunopharmacology, 10, (10) 1194 - 1199, 10 , Refereed
  • Dual mode of regulation of cell division cycle 25 a protein by TRB3, Satoshi Sakai, Nobumichi Ohoka, Kikuo Onozaki, Masatoshi Kitagawa, Makoto Nakanishi, Hidetoshi Hayashi, Biological and Pharmaceutical Bulletin, 33, (7) 1112 - 1116, 07 , Refereed
  • IL-1-induced ERK1/2 activation up-regulates p21Waf1/Cip1 protein by inhibition of degradation via ubiquitin-independent pathway in human melanoma cells A375, Tomohiro Arakawa, Hidetoshi Hayashi, Saotomo Itoh, Takemasa Takii, Kikuo Onozaki, Biochemical and Biophysical Research Communications, 392, (3) 369 - 372, 02 , Refereed
  • Anaphase-promoting complex/cyclosome-cdh1 mediates the ubiquitination and degradation of TRB3, Nobumichi Ohoka, Satoshi Sakai, Kikuo Onozaki, Makoto Nakanishi, Hidetoshi Hayashi, Biochemical and Biophysical Research Communications, 392, (3) 289 - 294, 02 , Refereed
  • [Interleukin-1(IL-1) alpha, beta, IL-1 receptor (IL-1R), IL-1 receptor antagonist (IL-1ra)]., Hidetoshi Hayashi, Kikuo Onozaki, Nippon Rinsho. Japanese Journal of Clinical Medicine, 68, ( Suppl 7) 62 - 66, 01
  • An environmental sensor, TRPV4 is a novel regulator of intracellular Ca2+ in human synoviocytes, Yuka Itoh, Noriyuki Hatano, Hidetoshi Hayashi, Kikuo Onozaki, Keiji Miyazawa, Katsuhiko Muraki, American Journal of Physiology - Cell Physiology, 297, (5) C1082 - C1090, 11 , Refereed
  • Two mechanistically and temporally distinct NF-κB activation pathways in IL-1 signaling, Kohsuke Yamazaki, Jin Gohda, Atsuhiro Kanayama, Yusei Miyamoto, Hiroaki Sakurai, Masahiro Yamamoto, Shizuo Akira, Hidetoshi Hayashi, Bing Su, Jun Ichiro Inoue, Science Signaling, 2, (93) , 10 , Refereed
  • Gene expression profiling identifies a role for CHOP during inhibition of the mitochondrial respiratory chain, Fumihiro Ishikawa, Takashi Akimoto, Haruka Yamamoto, Yuri Araki, Toshihiko Yoshie, Kazunori Mori, Hidetoshi Hayashi, Kiyoshi Nose, Motoko Shibanuma, Journal of Biochemistry, 146, (1) 123 - 132, 07 , Refereed
  • The orphan nuclear receptor RORα restrains adipocyte differentiation through a reduction of C/EBPβ activity and perilipin gene expression, Nobumichi Ohoka, Shogo Kato, Yu Takahashi, Hidetoshi Hayashi, Ryuichiro Sato, Molecular Endocrinology, 23, (6) 759 - 771, 06 , Refereed
  • TRB3 suppresses adipocyte differentiation by negatively regulating PPARγ transcriptional activity, Yu Takahashi, Nobumichi Ohoka, Hidetoshi Hayashi, Ryuichiro Sato, Journal of Lipid Research, 49, (4) 880 - 892, 04 , Refereed
  • Contribution of extracellular signal-regulated kinases to the IL-1-induced growth inhibition of human melanoma cells A375, Tomohiro Arakawa, Takanori Yamamura, Takayuki Hattori, Hidetoshi Hayashi, Akiko Mori, Ayako Yoshida, Chiharu Uchida, Masatoshi Kitagawa, Kikuo Onozaki, International Immunopharmacology, 8, (1) 80 - 89, 01 , Refereed
  • The interaction with Sp1 and reduction in the activity of histone deacetylase 1 are critical for the constitutive gene expression of IL-1α in human melanoma cells, Kazuaki Enya, Hidetoshi Hayashi, Takemasa Takii, Nobumichi Ohoka, Shinya Kanata, Takashi Okamoto, Kikuo Onozaki, Journal of Leukocyte Biology, 83, (1) 190 - 199, 01 , Refereed
  • Critical and functional regulations of CHOP (C/EBP homologous protein) through the N-terminal portion., Ohoka N, Hattori T, Kitagawa M, Onozaki K, Hayashi H, The Journal of Biological Chemistry, 282, (49) 35687 - 35694, 12 , Refereed
  • Cigarette smoke condensate upregulates the gene and protein expression of proinflammatory cytokines in human fibroblast-like synoviocyte line, Miki Shizu, Yuka Itoh, Ryohei Sunahara, Satomi Chujo, Hidetoshi Hayashi, Yuko Ide, Takemasa Takii, Masaya Koshiko, Sang Woon Chung, Kazuichi Hayakawa, Keiji Miyazawa, Kunitaka Hirose, Kikuo Onozaki, Journal of Interferon and Cytokine Research, 28, (8) 509 - 521, 08 , Refereed
  • A novel regulatory mechanism of the Bone Morphogenetic Protein (BMP) signaling pathway involving the carboxyl-terminal tail domain of BMP type II receptor, Chan MC, Nguyen PH, Davis BN, Ohoka N, Hayashi H, Du K, Lagna G, Hata A, Molecular and Cellular Biology, 27, (16) 5776 - 5789, 08 , Refereed
  • Dihydrotestosterone inhibits tumor necrosis factor α induced interleukin-1α mRNA expression in rheumatoid fibroblast-like synovial cells, Yuka Itoh, Hidetoshi Hayashi, Jian Xu, Takemasa Takii, Keiji Miyazawa, Hiroyoshi Ariga, Tohru Akahoshi, Yuko Waguri-Nagaya, Takanobu Otsuka, Takashi Okamoto, Kikuo Onozaki, Biological and Pharmaceutical Bulletin, 30, (6) 1140 - 1143, 06 , Refereed
  • Cell-cycle-dependent regulation of Ca2+-activated K+ channel in Jurkat T-lymphocyte., Morimoto T, Ohya S, Hayashi H, Onozaki K, Imaizumi Y, Journal of pPharmacological Sciences, 104, (1) 94 - 98, 05 , Refereed
  • Synthesis of glycosylated human tumor necrosis factor α coupled with N-acetylneuraminic acid, Akiko Hayashi, Taku Chiba, Hidetoshi Hayashi, Satoshi Sasayama, Toshiyuki Ishiguro, Kikuo Onozaki, Cancer Immunology, Immunotherapy, 56, (4) 545 - 553, 04 , Refereed
  • N-acetylneuraminic acid coupled human recombinant TNFα exhibits enhanced anti-tumor activity against Meth-A fibrosarcoma and reduced toxicity, Akiko Hayashi, Hidetoshi Hayashi, Taku Chiba, Satoshi Sasayama, Kikuo Onozaki, Cancer Immunology, Immunotherapy, 56, (4) 555 - 562, 04 , Refereed
  • 17β-estradiol induces IL-1α gene expression in rheumatoid fibroblast-like synovial cells through estrogen receptor α (ERα) and augmentation of transcriptional activity of Sp1 by dissociating histone deacetylase 2 from ERα, Yuka Itoh, Hidetoshi Hayashi, Keiji Miyazawa, Soichi Kojima, Tohru Akahoshi, Kikuo Onozaki, Journal of Immunology, 178, (5) 3059 - 3066, 03 , Refereed
  • Smad3 is acetylated by p300/CBP to regulate its transactivation activity., Inoue Y, Itoh Y, Abe K, Okamoto T, Daitoku H, Fukamizu A, Onozaki K, Hayashi H., 26, (4) 500 - 508, 06 , Refereed
  • CCAAT/enhancer-binding protein homologous protein (CHOP) regulates osteoblast differentiation., Shirakawa K, Maeda S, Gotoh T, Hayashi M, Shinomiya K, Ehata S, Nishimura R, Mori M, Onozaki K, Hayashi H, Uematsu S, Akira S, Ogata E, Miyazono K, Imamura T, Molecular and Cellular Biology, 26, (16) 6105 - 6116, 08 , Refereed
  • Estrogen stimulates transcription of human immunodeficiency virus type 1 (HIV-1), Daichi Katagiri, Hidetoshi Hayashi, Ann Florence B. Victoriano, Takashi Okamoto, Kikuo Onozaki, International Immunopharmacology, 6, (2) 170 - 181, 02 , Refereed
  • Transcriptional induction of Smurf2 ubiquitin ligase by TGF-β, Naro Ohashi, Tatsuo Yamamoto, Chiharu Uchida, Akashi Togawa, Hirotaka Fukasawa, Yoshihide Fujigaki, Sayuri Suzuki, Kyoko Kitagawa, Takayuki Hattori, Toshiaki Oda, Hidetoshi Hayashi, Akira Hishida, Masatoshi Kitagawa, FEBS Letters, 579, (12) 2557 - 2563, 05 , Refereed
  • TRB3, a novel ER stress inducible gene, is induced via ATF4-CHOP pathway and involved in CHOP dependent cell death., Ohoka N, Yoshii S, Hattori T, Onozaki K, Hayashi H, EMBO Journal, 24, (6) 1243 - 1255, 03 , Refereed
  • Interleukin-1 (IL-1) alpha, beta, IL-1 receptor, IL-1 receptor antagonist (IL-1ra), Hidetoshi Hayashi, Kikuo Onozaki, Nippon rinsho. Japanese journal of clinical medicine, 63, (Suppl 8) 60 - 64, 01
  • TGF-β down-regulates IL-1α-induced TLR2 expression in murine hepatocytes., Matsumura T, Hayashi H, Takii T, Thorn CF, Whitehead AS, Inoue JI, Onozaki K, Journal of leukocyte biology, 75, (6) 1056 - 1061, 06 , Refereed
  • Polycyclic aromatic hydrocarbon increases mRNA level for interleukin 1 beta in human fibroblast-like synoviocyte line via aryl hydrocarbon receptor, Ayako Tamaki, Hidetoshi Hayashi, Hironori Nakajima, Takemasa Takii, Daichi Katagiri, Keiji Miyazawa, Kunitaka Hirose, Kikuo Onozaki, Biological and Pharmaceutical Bulletin, 27, (3) 407 - 410, 03 , Refereed
  • A potential live vector, foamy virus, directed intra-cellular expression of ovine interferon-τ exhibited the resistance to HIV infection, Yoichi Fujii, Yasunori Murase, Kaori Otake, Yasuko Yokota, Shinya Omoto, Hidetoshi Hayashi, Hidetika Okada, Noriko Okada, Masahiro Kawai, Harumi Okuyama, Kazuhiko Imakawa, Journal of Veterinary Medical Science, 66, (2) 115 - 121, 02 , Refereed
  • Contribution of the Constitutive and Inducible Degradation of Smad3 by the Ubiquitin-Proteasome Pathway to Transforming Growth Factor-β Signaling, Yasumichi Inoue, Masatoshi Kitagawa, Kikuo Onozaki, Hidetoshi Hayashi, Journal of Interferon and Cytokine Research, 24, (4) 43 - 54, 01 , Refereed
  • TRAF6-NF-κB pathway is essential for interleukin-1-induced TLR2 expression and its functional response to TLR2 ligand in murine hepatocytes, Takayuki Matsumura, Takahiro Degawa, Takemasa Takii, Hidetoshi Hayashi, Takashi Okamoto, Jun Ichiro Inoue, Kikuo Onozaki, Immunology, 109, (1) 127 - 136, 05 , Refereed
  • C/EBP homologous protein (CHOP) up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform, Takayuki Hattori, Nobumichi Ohoka, Hidetoshi Hayashi, Kikuo Onozaki, FEBS Letters, 541, (1-3) 33 - 39, 04 , Refereed
  • Synthesis and biological activities in vitro and in vivo of glycosylated human interleukin-1α, neoglyco IL-1α, coupled with N-acetylneuraminyl-galactose, Masaaki Ootsubo, Taku Chiba, Yutaka Kobayashi, Hidetoshi Hayashi, Akiko Hayashi, Kikuo Onozaki, Glycoconjugate Journal, 20, (2) 119 - 131, 03 , Refereed
  • C/EBP family transcription factors are degraded by the proteasome but stabilized by forming dimer., Hattori T, Ohoka N, Inoue Y, Hayashi H, Onozaki K, Oncogene, 22, (9) 1273 - 1280, 03 , Refereed
  • Multiple mechanisms involved in the inhibition of proinflammatory cytokine production from human monocytes by N-(p-coumaroyl)serotonin and its derivatives, Takemasa Takii, Sachiko Kawashima, Taku Chiba, Hidetoshi Hayashi, Masaru Hayashi, Hiroyuki Hiroma, Hiroaki Kimura, Yoshitaka Inukai, Yayoi Shibata, Akito Nagatsu, Jinnsaku Sakakibara, Yasukazu Oomoto, Kunitaka Hirose, Kikuo Onozaki, International Immunopharmacology, 3, (2) 273 - 277, 02 , Refereed
  • TGFβ down-regulates IFN-γ production in IL-18 treated NK cell line LNK5E6, Hidetoshi Hayashi, Yasumichi Inoue, Hiroko Tsutsui, Haruki Okamura, Kenji Nakanishi, Kikuo Onozaki, Biochemical and Biophysical Research Communications, 300, (4) 980 - 985, 01 , Refereed
  • 3-Methylcholanthrene activates human immunodeficiency virus type 1 replication via aryl hydrocarbon receptor, Hirokazu Ohata, Toshifumi Tetsuka, Hidetoshi Hayashi, Kikuo Onozaki, Takashi Okamoto, Microbiology and Immunology, 47, (5) 363 - 370, 01 , Refereed
  • CHOP, a basic leucine zipper transcriptional factor, contributes to the antiproliferative effect of IL-1 on A375 human melanoma cells through augmenting transcription of IL-6, T. Hattori, S. Itoh, H. Hayashi, T. Chiba, T. Takii, K. Yoshizaki, K. Onozaki, Journal of Interferon and Cytokine Research, 21, (5) 323 - 332, 08 , Refereed
  • Activation of two distinct anti-proliferative pathways, apoptosis and p38 MAP kinase-dependent cell cycle arrest, by tumor necrosis factor in human melanoma cell line A375, T. Hattori, H. Hayashi, T. Chiba, K. Onozaki, European Cytokine Network, 12, (2) 244 - 252, 06 , Refereed
  • Glycosylated human interleukin-1α, neoglyco IL-1α, coupled with N-acetylneuraminic acid exhibits selective activities in vivo and altered tissue distribution, S. Sasayama, K. Moriya, T. Chiba, T. Matsumura, H. Hayashi, A. Hayashi, K. Onozaki, Glycoconjugate Journal, 17, (6) 353 - 359, 12 , Refereed
  • Endotoxin and cytokine regulation of toll-like receptor (TLR) 2 and TLR4 gene expression in murine liver and hepatocytes, T. Matsumura, A. Ito, T. Takii, H. Hayashi, K. Onozaki, Journal of Interferon and Cytokine Research, 20, (10) 915 - 921, 10 , Refereed
  • Synthesis of glycosylated human interleukin-1α, neoglyco IL-1α, coupled with N-acetylneuraminic acid, Taku Chiba, Kayoko Moriya, Sachi Nabeshima, Hidetoshi Hayashi, Yutaka Kobayashi, Satoshi Sasayama, Kikuo Onozaki, Glycoconjugate Journal, 16, (9) 499 - 505, 12 , Refereed
  • In vitro biological activities of glycosylated human interleukin-1α, neoglyco IL-1α, coupled with N-acetylneuraminic acid, Kayoko Moriya, Taku Chiba, Sachi Nabeshima, Hidetoshi Hayashi, Kikuo Onozaki, Glycoconjugate Journal, 16, (9) 563 - 568, 12 , Refereed
  • Tyrosine kinase is essential for the constitutive expression of type I interleukin-1 receptor in human fibroblast cells, Takemasa Takii, Atsushi Ito, Sachiko Kawashima, Ayako Ninomiya, Takayuki Matsumura, Hidetoshi Hayashi, Kikuo Onozaki, European Cytokine Network, 10, (2) 237 - 245, 07 , Refereed
  • Antiproliferative effect of IL-1 is mediated by p38 mitogen-activated protein kinase in human melanoma cell A375, Saotomo Itoh, Takayuki Hattori, Hidetoshi Hayashi, Yukiko Mizutani, Makoto Todo, Takemasa Takii, De Yang, John C. Lee, Senya Matsufuji, Yasuko Murakami, Taku Chiba, Kikuo Onozaki, Journal of Immunology, 162, (12) 7434 - 7440, 06 , Refereed
  • Interleukin-1 (IL-1), IL-1 receptor, IL-1 receptor antagonist (IL-1ra), H. Hayashi, K. Onozaki, Nippon rinsho. Japanese journal of clinical medicine, 57 Suppl, 769 - 774, 01 01
  • Molecular analysis of constitutive IL-1α gene expression in human melanoma cells: Autocrine stimulation through NF-κB activation by endogenous IL-1α, Hiroaki Kimura, Yoshitaka Inukai, Takemasa Takii, Yasuji Furutani, Yayoi Shibata, Hidetoshi Hayashi, Shinsaku Sakurada, Takashi Okamoto, Jun Ichiro Inoue, Yasukazu Oomoto, Kikuo Onozaki, Cytokine, 10, (11) 872 - 879, 01 , Refereed
  • Acquired resistance to the anti-proliferative effect of interleukin-1 and interleukin-6 is a recessive phenotype in A375 human melanoma cells, S. Itoh, H. Hayashi, D. Yang, T. Takii, K. Onozaki, Melanoma Research, 7, (6) 455 - 462, 12 , Refereed
  • Resistance to IL-1 anti-proliferative effect, accompanied by characteristics of advanced melanoma, permits invasion of human melanoma cells in vitro, but not metastasis in the nude mouse, Hidetoshi Hayashi, Reiko Shimizu, Kaori Fujii, Saotomo Itoh, De Yang, Kikuo Onozaki, International Journal of Cancer, 71, (3) 416 - 421, 06 , Refereed
  • Dharmendra Antigen but not Integral M. leprae is an Efficient Inducer of Immunostimulant Cytokine Production by Human Monocytes, and M. leprae Lipids Inhibit the Cytokine Production, Chikako Nakamura, Yasuo Fukutomi, Yoshiko Kashiwabara, Yasukazu Oomoto, Miyae Kojima, Hidetoshi Hayashi, Kikuo Onozaki, International Journal of Leprosy and Other Mycobacterial Diseases, 65, (1) 63 - 72, 03 , Refereed
  • Interleukin-1-induced growth inhibition of human melanoma cells Interleukin-1 induced antizyme expression is responsible for ornitine decarboxylase activity down-regulation., Yang D, Hayashi H, Takii T, Mizutani Y, Inukai Y, Onozaki K., Journal of Biological Chemistry, 272, (6) 3376 - 3383, 02 , Refereed
  • TbetaRI phosphorylation of Smad2 on serine465 and serine467 is required for Smad2-Smad4 complex formation and signaling., Abdollah S, Macías-Silva M, Tsukazaki T, Hayashi H, Attisano L, Wrana JL., Journal of Biological Chemistry, 272, (44) 27678 - 27685, 10 , Refereed
  • The MAD-related protein Smad7 associates with the TGF-beta receptor and function as an antagonist of TGF-beta signaling., Hayashi H, Abdollah S, Qiu Y, Cai J, Xu YY, Grinnell BW, Richardson, MA, Topper JN, Gimbrone MA Jr, Wrana JL, Falb D, Cell, 89, (7) 1165 - 1173, 06 , Refereed
  • Interleukin 1 (IL-1) production is not essential for acquired resistance of human A375 melanoma cells to anti-proliferative effect of IL-1, Saotomo Itoh, Hidetoshi Hayashi, Naoko Watanabe, Yoshiro Kobayashi, Takemasa Takii, Kikuo Onozaki, International Journal of Cancer, 65, (6) 805 - 811, 03 , Refereed
  • Molecular cloning of human antizyme cDNA, De Yang, Takemasa Takii, Hidetoshi Hayashi, Saotomo Itoh, Masaru Hayashi, Kikuo Onozaki, Biochemistry and Molecular Biology International, 38, (5) 957 - 964, 01
  • Identification of a novel growth-promoting factor with a wide target cell spectrum from various tumor cells as catalase, Tomomi Miyamoto, Masaru Hayashi, Akihiko Takeuchi, Takayuki Okamoto, Sachiko Kawashima, Takemasa Takii, Hidetoshi Hayashi, Kikuo Onozaki, Journal of Biochemistry, 120, (4) 725 - 730, 01 , Refereed
  • A high α-linolenate diet has no significant effect on interleukin-1 production but suppresses the loss of body weight in lipopolysaccharide-treated mice, Shiro Watanabe, Hidenori Ito, Tetsuyuki Kobayashi, Hidetoshi Hayashi, Kikuo Onozaki, Harumi Okuyama, Journal of Nutritional Immunology, 3, 61 - 70, 07
  • Differential Effects of Prostaglandin E1 and Prostaglandin E2 on Growth and Differentiation of Murine Myeloid Leukemic Cell Line, M1, Hiroshi Sawamura, Hidetoshi Hayashi, Kikuo Onozaki, Microbiology and Immunology, 39, (10) 809 - 815, 01 , Refereed
  • Transfection of human melanoma cells with type I interleukin-1 (IL-1) receptor cdna rendered them IL-1-responsive and revealed the importance of ODC activity down-regulation in IL-1-induced growth inhibition, De Yang, Hidetoshi Hayashi, Yoshio Hiyama, Takemasa Takii, Kikuo Onozaki, Journal of Biochemistry, 118, (4) 802 - 809, 01 , Refereed
  • Ubiquitous interleukin-1α in fetal bovine serum may mislead the experimental results in vitro, S. Hida, A. Takeuchi, H. Hayashi, T. Yano, S. J. Hopkins, K. Onozaki, European Cytokine Network, 6, (2) 121 - 126, 01 , Refereed
  • Glycosylated Human Recombinant Interleukin-1α, Neo Interleukin-1α, with D-Mannose Dimer Exhibits Selective Activities in Vivo, Yutaka Takei, Taku Chiba, Kaoru Wada, Hidetoshi Hayashi, Masaaki Yamada, Junji Kuwashima, Kikuo Onozaki, Journal of Interferon and Cytokine Research, 15, (8) 713 - 719, 01 , Refereed
  • Type I and Type II Interferons Upregulate Functional Type I Interleukin-1 Receptor in a Human Fibroblast Cell Line TIG-1, Takemasa Takii, Noriyuki Niki, de Yang, Hiroaki Kimura, Atsushi Ito, Hidetoshi Hayashi, Kikuo Onozaki, Journal of Interferon and Cytokine Research, 15, (12) 1065 - 1073, 01 , Refereed
  • A human erythrocyte-derived growth promoting factor with a wide target cell spectrum: identification as catalase., Takeuchi A, Miyamoto T, Yamaji K, Masuho Y, Hayashi M, Hayashi H, Onozaki K., Cancer Research, 55, (7) 1586 - 1589, 04 , Refereed
  • Synthesis of immunosuppressive neoglycoproteins: Bovine serum albumin coupled with 8-(hydrazino-carbonyl)octyl 4- OR 6-O-α- D-MAnnopyranosyl-a-d-mannopyranoside, Kaoru Wada, Taku Chiba, Yutaka Takei, Hideko Ishihara, Hidetoshi Hayashi, Kikuo Onozaki, Journal of Carbohydrate Chemistry, 13, 941 - 965, 10
  • Development of glycosylated human interleukin-1α, neoglyco IL-1α, coupled with D-mannose dimer: Synthesis and biological activities in vitro, Y. Takei, K. Wada, T. Chiba, H. Hayashi, H. Ishihara, K. Onozaki, Lymphokine and Cytokine Research, 13, (4) 265 - 270, 01 , Refereed
  • Novel growth promoting activity with a wide target cell spectrum is present in extracts of various cell types, T. Miyamoto, A. Takeuchi, H. Hayashi, K. Onozaki, Biochemistry and Molecular Biology International, 32, (5) 973 - 981, 01 , Refereed
  • Interleukin-1 down-regulates type I interleukin 1 receptor mRNA expression in a human fibroblast cell line TIG-1 in the absence of prostaglandin E2 synthesis, T. Takii, H. Hayashi, T. Marunouchi, K. Onozaki, Lymphokine and Cytokine Research, 13, (3) 213 - 219, 01 , Refereed
  • Differences in interleukin 1 (IL-1), IL-6, tumor necrosis factor and IL-1 receptor antagonist production by human monocytes stimulated with muramyl dipeptide (MDP) and its stearoyl derivative, romurtide, Katsuya Suzuki, Keiko Torii, Shigeaki Hida, Hidetoshi Hayashi, Yoshio Hiyama, Yasukazu Oomoto, Takemasa Takii, Taku Chiba, Kikuo Onozaki, Immunopharmacology, 28, (1) 31 - 38, 01 , Refereed
  • Resistance to the anti‐proliferative effect of IL‐1 on human melanoma cell line is associated with endogenous production of IL‐1 and IL‐6, Makoto Araki, Taishi Yano, Hidetoshi Hayashi, Takemasa Takii, Katsuya Suzuki, Kikuo Onozaki, International Journal of Cancer, 56, (2) 275 - 280, 01 , Refereed
  • Purification and characterization of a human erythrocyte-derived growth factor with a wide target cell spectrum., Takeuchi A, Miyamoto T, Hayashi H, Tomita M, Onozaki K., Journal of Biological Chemistry, 269, (40) 24935 - 24940, 10 , Refereed
  • Differential production of interleukin 1 (IL-1), IL-6, tumor necrosis factor, and IL-1 receptor antagonist by human monocytes stimulated with Mycobacterium leprae and M. bovis BCG, K. Suzuki, Y. Fukutomi, M. Matsuoka, K. Torii, H. Hayashi, T. Takii, Y. Oomoto, K. Onozaki, International Journal of Leprosy, 61, (4) 609 - 618, 12 , Refereed
  • Development of neoglycoproteins conjugated with natural oligosaccharides through carboxyl residues of proteins and its application to recombinant human interleukin 1, H. Ishihara, T. Chiba, A. Takeuchi, H. Hayashi, Y. Takei, T. Takii, K. Onozaki, Biochemistry and Molecular Biology International, 31, (3) 527 - 535, 01 , Refereed
  • Human myelomonocytic cellline THP-1 produces a novel growth-promoting factor with a wide target cell spectrum., 53, (8) 1871 - 1876, 04 , Refereed
  • Three Types of Membranous ATPase on Rat Liver Lysosomes, Hidetoshi Hayashi, Kunizo Arai, Osamu Sato, Akiyoshi Shimaya, Yoshimichi Sai, Shoji Ohkuma, Chemical and Pharmaceutical Bulletin, 40, (10) 2783 - 2786, 01 , Refereed
  • Interleukin‐1 up‐regulates transcription of its own receptor in a human fibroblast cell line TIG‐1: role of endogenous PGE2 and cAMP, Takemasa Takii, Tohru Akahoshi, Koichi Kato, Hidetoshi Hayashi, Tohru Marunouchi, Kikuo Onozaki, European Journal of Immunology, 22, (5) 1221 - 1227, 01 , Refereed
  • Biological response of guinea pig peritoneal macrophages to platelet-activating factor, Hidetoshi Hayashi, Ichiro Kudo, Shoshichi Nojima, Keizo Inoue, Lipids, 26, (12) 1193 - 1199, 12 , Refereed
  • Guinea pig bone marrow cells treated with platelet-activating factor generate factor(s) which affects their DNA synthesis and microbicidal activity, Ichiro Kudo, Toshiyuki Kato, Hidetoshi Hayashi, Ryohei Yanoshita, Koichi Ikizawa, Hiroko Uda, Keizo Inoue, Lipids, 26, (12) 1065 - 1070, 12 , Refereed
  • Effect of dietary α-linolenate/linoleate balance on lipopolysaccharide-induced tumor necrosis factor production in mouse macrophages, Shiro Watanabe, Hidetoshi Hayashi, Kikuo Onozaki, Harumi Okuyama, Life Sciences, 48, (21) 2013 - 2020, 01 , Refereed
  • Antiproliferative effect of interleukin 1 (IL-1) on tumor cells: G0-G1 arrest of a human melanoma cell line by IL-1, Yoshihiro Morinaga, Hidetoshi Hayashi, Akihiko Takeuchi, Kikuo Onozaki, Biochemical and Biophysical Research Communications, 173, (1) 186 - 192, 11 , Refereed
  • Membranous atpases and transport functions of lysosomes, Shoji Ohkuma, Hidetoshi Hayashi, Kunizo Arai, Sakae Shimizu, Yoshinori Moriyama, Kiyohisa Iinuma, Yoshinobu Morimoto, Hirokazu Watanabe, Acta Histochemica et Cytochemica, 23, 41 - 51, 01 , Refereed
  • Augmentation of DNA synthesis in guinea pig bone marrow cells by platelet-activating factor (PAF), Toshiyuki Kato, Ichiro Kudo, Hidetoshi Hayashi, Kikuo Onozaki, Keizo Inoue, Biochemical and Biophysical Research Communications, 157, (2) 563 - 568, 12 , Refereed
  • A novel bioaction of PAF: Induction of microbicidal activity in guinea pig bone marrow cells, Hidetoshi Hayashi, Ichiro Kudo, Toshiyuki Kato, Ryushi Nozawa, Shoshichi Nojima, Keizo Inoue, Lipids, 23, (12) 1119 - 1124, 12 , Refereed
  • Induction by platelet activating factor of candidacidal activity in guinea pig bone marrow cells, I. Kudo, H. Hayashi, T. Kato, R. Nozawa, S. Nojima, K. Inoue, International Journal of Immunopharmacology, 10, 01 01
  • Antitumor activity of synthetic alkylphospholipids with or without PAF activity, Ichiro Kudo, Shoshichi Nojima, Hyeun Wook Chang, Ryohei Yanoshita, Hidetoshi Hayashi, Eri Kondo, Hiroaki Nomura, Keizo Inoue, Lipids, 22, (11) 862 - 867, 11 , Refereed
  • Activation of guinea pig peritoneal macrophages by platelet-activating factor in free form or incorporated into liposomes., K. Inoue, H. Hayashi, I. Kudo, S. Tsushima, H. Nomura, S. Nojima, Advances in prostaglandin, thromboxane, and leukotriene research, 15, 711 - 713, 12 , Refereed
  • Activation of Guinea pig peritoneal macrophages by platelet activating factor (PAF) and its agonists, Hidetoshi Hayashi, Ichiro Kudo, Keizo Inoue, Kikuo Onozaki, Susumu Tsushima, Hiroaki Nomura, Shoshichi Nojima, Journal of Biochemistry, 97, (6) 1737 - 1745, 01 , Refereed
  • Macrophage activation by PAF incorporated into dipalmitoylphosphatidylcholine-cholesterol liposomes, Hidetoshi Hayashi, Ichiro Kudo, Keizo Inoue, Hiroaki Nomura, Shoshichi Nojima, Journal of Biochemistry, 97, (4) 1255 - 1258, 01 , Refereed
  • The ubiquitin-specific protease USP17 prevents cellular senescence by stabilizing the methyltransferase SET8 and transcriptionally repressing p21., Keishi Fukuura, Yasumichi Inoue, Chiharu Miyajima, Shin Watanabe, Muneshige Tokugawa, Daisuke Morishita, Nobumichi Ohoka, Masayuki Komada, Hidetoshi Hayashi, Journal of Biological Chemistry, Refereed

Conference Activities & Talks

  • TGF-β induces p53/Smads complex formation in the PAI-1 promoter to activate transcription, Yuki Kawarada, Yasumichi Inoue, Fumihiro Kawasaki, Keishi Fukuura, Koichi Sato, Takahito Tanaka, Yuka Itoh, Hidetoshi Hayashi, Scientific Reports,   2016 10 19 , © The Author(s) 2016. Transforming growth factor β (TGF-β) signaling facilitates tumor development during the advanced stages of tumorigenesis, but induces cell-cycle arrest for tumor suppression during the early stages. However, the mechanism of functional switching of TGF-β is still unknown, and it is unclear whether inhibition of TGF-β signaling results amelioration or exacerbation of cancers. Here we show that the tumor suppressor p53 cooperates with Smad proteins, which are TGF-β signal transducers, to selectively activate plasminogen activator inhibitor type-1 (PAI-1) transcription. p53 forms a complex with Smad2/3 in the PAI-1 promoter to recruit histone acetyltransferase CREB-binding protein (CBP) and enhance histone H3 acetylation, resulting in transcriptional activation of the PAI-1 gene. Importantly, p53 is required for TGF-β-induced cytostasis and PAI-1 is involved in the cytostatic activity of TGF-β in several cell lines. Our results suggest that p53 enhances TGF-β-induced cytostatic effects by activating PAI-1 transcription, and the functional switching of TGF-β is partially caused by p53 mutation or p53 inactivation during cancer progression. It is expected that these findings will contribute to optimization of TGF-β-targeting therapies for cancer.

Research Grants & Projects

  • 腫瘍細胞に高発現するdecoy kinase TRB3の機能解析,   2006 04  - 2008 03 , Hidetoshi Hayashi
  • ストレスセンサー分子TRBによる脂肪細胞の機能制御,   2006 04  - 2008 03
  • 新規kinase様タンパクTRBファミリーによる細胞の増殖・分化の制御,   2006 04  - 2008 03
  • 外的ストレスにおけるCHOP依存的な細胞死の分子基盤,   2004 04  - 2006 03
  • 内分泌攪乱物質による免疫応答の攪乱作用,   2002 04  - 2004 03
  • TGFβの情報伝達分子Smadタンパクの分解によるシグナル伝達の制御機構,   2001 04  - 2003 03
  • 担癌状態におけるTGFβを介した免疫抑制作用の基礎的研究,   1999 04  - 2001 03
  • インタ-ロイキン1の構成的産生ヒトメラノ-マ細胞の癌原性及び転移能に関する研究,   1994 04  - 1996 03
  • インタ-ロイキン1の細胞増殖阻害作用に対する耐性獲得機構の解析,   1994 04  - 1996 03

Social Contribution Activities Information

Social Contribution

  • 環境省環境研究企画委員会分科会委員, Advisor, 環境省,   2004 04 01  - 2013 03 31
  • 名古屋市廃棄物処理施設専門委員会委員, Advisor, 名古屋市,   2002 08 01  - 2014 07 31
  • 薬学共用試験センター試験統括委員会委員, Advisor,   2012 09 01  - 現在


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