Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Date (from‐to) : 2011/04 -2015/03
Author : NAKAZAWA takahiro; OHARA Hirotaka; INAGAKI Hiroshi; HAYSHI Kaduki; NAITOH Itaru
We analyzed the immunoglobulin V heavy chain (VH) gene rearrangement and somatic hypermutation of lymphoid cells infiltrating in AIP (n=3), using obstructive pancreatitis (n=3) as non-autoimmune control. DNA was extracted from the affected inflammatory lesions. After PCR amplification of rearranged VH genes, the clones were subcloned. 100 recombinant clones were randomly picked-up in each case and sequenced. Monoclonal VH rearrangement was not detected in any cases examined. As compared with obstructive pancreatitis, there was no VH family or VH fragment specific to AIP. However, rates of unmutated VH fragments in AIP (25%) were higher than that in obstructive pancreatitis (5.1%) (P=0.0039).
Conclusions: Some autoantibodies encoded by germline or less mutated VH genes may fail to be eliminated, and could play a role in the development of AIP.