Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2012/04 -2015/03
Author : KEIKO Yamada
In this study, we aimed to developed specific antibodies against resistant-factor for rapid detection of drug-resistant microorganisms. In this study, target molecules are key proteins of drug-resistance, 1) PBP2a of methicillin resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (MRCNS), 2) IMP-1 of carbapenem-resistant bacteria, and 3) oseltamivir- and peramivir-resistant type neuraminidase of influenza virus. I have prepared specific antibodies for them, and they showed the specificity with recombinant proteins and resistant/susceptible microorganisms. I constructed immunochromatography assay system using specific anti-PBP2a chicken IgY. It was revealed that it could detected MRSA and MRCNS directly from mimicked blood-cultured bottle in about 3 hours.