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KUBOTA Yasue

FacultyGraduate School of Nursing Physiology
PositionProfessor
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Last Updated :2020/07/02

Researcher Profile and Settings

Education

  • Nagoya City University

Degree

  • 名古屋市立大学医学部/博士(医学)

Association Memberships

  • JAPAN SOCIETY FOR REPRODUCTIVE MEDICINE
  • THE JAPAN SOCIETY FOR ORIENTAL MEDICINE
  • THE JAPANESE UROLOGICAL ASSOCIATION
  • JAPANESE SOCIETY OF GERIATRIC UROLOGY
  • THE JAPAN SOCIETY FOR MEDICAL EDUCATION

Research Activities

Published Papers

  • A kit ligand, stem cell factor as a possible mediator inducing overactive bladder., Kubota Yasue, Hamakawa T, Osaga S, Okada A, Hamamoto S, Kawai N, Kohri K, Yasui T, 37, (4) 1258 - 1265, 04 , Refereed
  • Adrenal Neuroblastoma in an Adult: Effect of Radiotherapy on Local Progression after Surgical Removal., Kurokawa S, Mizuno K, Nakane A, Moritoki Y, Nishio H, Kamisawa H, Kubota Y, Okada A, Kawai N, Hayashi Y, Yasui T, Case reports in urology, 2016, Refereed
  • Novel effect of the inhibitor of mitochondrial cyclophilin D activation, N-methyl-4-isoleucine cyclosporin, on renal calcium crystallization, Kazuhiro Niimi, Takahiro Yasui, Atsushi Okada, Yasuhiko Hirose, Yasue Kubota, Yukihiro Umemoto, Noriyasu Kawai, Keiichi Tozawa, Kenjiro Kohri, INTERNATIONAL JOURNAL OF UROLOGY, 21, (7) 707 - 713, 07 , Refereed, Objectives: To experimentally evaluate the clinical application of N-methyl-4-isoleucine cyclosporin, a novel selective inhibitor of cyclophilin D activation. Methods: In vitro, cultured renal tubular cells were exposed to calcium oxalate monohydrate crystals and treated with N-methyl-4-isoleucine cyclosporin. The mitochondrial membrane was stained with tetramethylrhodamine ethyl ester perchlorate and observed. In vivo, Sprague-Dawley rats were divided into four groups: a control group, an ethylene glycol group (administration of ethylene glycol to induce renal calcium crystallization), a N-methyl-4-isoleucine cyclosporin group (administration of N-methyl-4-isoleucine cyclosporin) and an ethylene glycol + N-methyl-4-isoleucine cyclosporin group (administration of ethylene glycol and N-methyl-4-isoleucine cyclosporin). Renal calcium crystallization was evaluated using Pizzolato staining. Oxidative stress was evaluated using superoxide dismutase and 8-hydroxy-deoxyguanosine. Mitochondria within renal tubular cells were observed by transmission electron microscopy. Cell apoptosis was evaluated using cleaved caspase-3. Results: In vitro, calcium oxalate monohydrate crystals induced depolarization of the mitochondrial membrane potential, which was remarkably prevented by N-methyl-4-isoleucine cyclosporin. In vivo, ethylene glycol administration induced renal calcium crystallization, oxidative stress, mitochondrial collapse and cell apoptosis in rats, which were significantly prevented by N-methyl-4-isoleucine cyclosporin. Conclusions: Herein we first report a new treatment agent determining renal calcium crystallization through cyclophilin D activation.
  • Bladder neck sling suspension during robot-assisted radical prostatectomy to improve early return of urinary continence: a comparative analysis., Kojima Y, Hamakawa T, Kubota Y, Ogawa S, Haga N, Tozawa K, Sasaki S, Hayashi Y, Kohri K, Urology, 83, (3) 632 - 639, 03 , Refereed
  • Long-term follow-up of nephrotoxicity in rats administered both melamine and cyanuric acid., Yasui T, Kobayashi T, Okada A, Hamamoto S, Hirose M, Mizuno K, Kubota Y, Umemoto Y, Kawai N, Tozawa K, Gao B, Kohri K, BMC research notes, 7, 02 , Refereed
  • Kidney stone formation is positively associated with conventional risk factors for coronary heart disease in Japanese men., Ando R, Nagaya T, Suzuki S, Takahashi H, Kawai M, Okada A, Yasui T, Kubota Y, Umemoto Y, Tozawa K, Kohri K, The Journal of urology, 189, (4) 1340 - 1346, 04 , Refereed
  • Long-term survival of a patient with invasive signet-ring cell carcinoma of the urinary bladder managed by combined s-1 and Cisplatin adjuvant chemotherapy., Hamakawa T, Kojima Y, Naiki T, Kubota Y, Yasui T, Tozawa K, Hayashi Y, Kohri K, Case reports in urology, 2013, Refereed
  • Clinical impact of palliative treatment using octreotide for inoperable malignant bowel obstruction caused by advanced urological cancer., Kubota H, Taguchi K, Kobayashi D, Naruyama H, Hirose M, Fukuta K, Kubota Y, Yasui T, Yamada Y, Kohri K, Asian Pacific journal of cancer prevention : APJCP, 14, (12) 7107 - 7110, Refereed
  • Up-Regulation of alpha(1a) and alpha(1d)-Adrenoceptors in the Prostate by Administration of Subtype Selective alpha(1)-Adrenoceptor Antagonist Tamsulosin in Patients With Benign Prostatic Hyperplasia, Yoshiyuki Kojima, Shoichi Sasaki, Yasue Kubota, Makoto Imura, Nobuyuki Oda, Mamoru Kiniwa, Yutaro Hayashi, Kenjiro Kohri, JOURNAL OF UROLOGY, 186, (4) 1530 - 1536, 10 , Refereed, Purpose: We examined the change in alpha(1)-adrenoceptor subtype expression in the prostate due to chronic tamsulosin administration in a benign prostatic hyperplasia rat model and in patients. Materials and Methods: We measured alpha(1)-adrenoceptor subtype expression after tamsulosin administration in the prostate of the benign prostatic hyperplasia rat model using TaqMan (R) reverse transcriptase-polymerase chain reaction. We also measured expression before and after 12-week tamsulosin treatment in the prostate of patients with benign prostatic hyperplasia. We examined the correlation between the change in alpha(1)-adrenoceptor expression due to tamsulosin treatment and acute urinary retention during long-term followup. Results: The expression of alpha(1a) and alpha(1d)-adrenoceptors was significantly increased in dose dependent fashion by tamsulosin in the benign prostatic hyperplasia rat model. Median mRNA expression of subtypes alpha(1a) and alpha(1d)-adrenoceptors was 1.4 (IQR 0.6, 3.0) and 1.7 X 1,000 copies per 1 ng beta-actin (IQR 0.9, 2.4) before treatment, and 6.0 (IQR 2.0, 8.0) and 2.2 X 1,000 copies per 1 ng beta-actin (IQR 1.7, 3.6), respectively, after treatment. The expression of alpha(1a) and alpha(1d)-adrenoceptors significantly increased after tamsulosin treatment (p < 0.01 and < 0.05, respectively). This increase was observed in 10 patients in whom acute urinary retention did not develop during long-term followup but not in 4 in whom acute urinary tract retention developed. Conclusions: Tamsulosin up-regulated alpha(1a) and alpha(1d)-adrenoceptors, suggesting that it has clinical selectivity for alpha(1a) and alpha(1d)-adrenoceptors. Up-regulation of alpha(1)-adrenoceptors subtype expression is considered an adaptive response to chronic tamsulosin administration. The difference in the response to alpha(1)-adrenoceptors antagonists among patients may contribute to the diversity in the long-term efficiency of alpha(1)-adrenoceptor antagonists.
  • Characterization of alpha(1)-adrenoceptor Subtypes Mediating Contraction in Human Isolated Ureters, Shoichi Sasaki, Yoshitaka Tomiyama, Shinya Kobayashi, Yoshiyuki Kojima, Yasue Kubota, Kenjiro Kohri, UROLOGY, 77, (3) 762.e13 - 7, 03 , Refereed, OBJECTIVES To characterize the contractile functions of the alpha(1)-adrenoceptor (AR) subtypes present in the human ureter. METHODS Specimens were taken from patients with renal cancer ("upper ureters;" n = 51) or bladder cancer ("lower ureters;" n = 23) who had not been treated by chemotherapy, radiation therapy, or immunotherapy before surgery. Patients systemically taking an alpha(1)-AR agonist or antagonist were excluded from this study. The effects of alpha(1)-AR antagonists against phenylephrine (alpha(1)-AR agonist)-induced contractions were evaluated in human isolated ureteral preparations. RESULTS Pooled data from all ureters showed that phenylephrine (alpha(1)-AR agonist) induced a concentration-dependent tonic contraction (pD(2) value, 4.92 +/- 011). The phenylephrine-induced maximum contraction was significantly greater in lower ureters than in upper ones. Prazosin (non-selective alpha(1)-AR antagonist), silodosin (selective alpha(1A)-AR antagonist), and BMY-7378 (selective alpha(1D)-AR antagonist) all shifted the concentration-contractile response curve for phenylephrine to the right, the rank order of potencies in all ureters (pK(B) values) being silodosin (9.72 +/- 0.14) > prazosin (8.64 +/- 0.08) > BMY-7378 (7.04 +/- 0.14). The alpha(1A)-AR antagonist silodosin was thus much more potent than the other 2 antagonists. CONCLUSIONS Our results suggest that among alpha(1)-ARs, the alpha(1A) subtype plays the major role in contraction in the human ureter. UROLOGY 77: 762.e13-762.e17, 2011. Crown Copyright (C) 2011 Published by Elsevier Inc.
  • Role of KIT-positive interstitial cells of Cajal in the urinary bladder and possible therapeutic target for overactive bladder., Kubota Yasue, Kojima Yoshiyuki, Shibata Yasuhiro, Imura Makoto, Kohri Kenjiro, Sasaki Shoichi, Adv Urol, 816342, 2011

Research Grants & Projects

  • 過活動膀胱におけるKit受容体を標的とした新規分子標的治療薬の開発,   2013  - 2015
  • 過活動膀胱における膀胱粘膜下微小循環の変化と KIT 陽性間質細胞の役割,   2011  - 2012
  • 過活動膀胱の発症に関わるKIT-SCF遺伝子の一塩基遺伝子多型解析,   2009  - 2010
  • 正常および過活動膀胱におけるc-kit陽性間質細胞機能の研究
  • 過活動膀胱の膀胱興奮性におけるKit陽性細胞の役割

Social Contribution Activities Information

Social Contribution

  • 名古屋市女性会館主催講座(招聘講演), 名古屋市,  - 2015 05 19 , 「ワタシだけのヒミツ「泌尿器」の悩み」の講演
  • 名古屋市女性会館共催講座, 行政,   2012 11 17  - 2012 11 17 , 他人には言えない「泌尿器の悩み」について一般市民向けに講演した。
  • 女子力向上Bookへの寄稿, 民間企業,   2012 09 06  - 2012 09 06 , 女性のための漢方セミナーの際に配布される女子力向上Bookに漢方を処方する医師として寄稿した。
  • テレビ愛知「健康ワンダフル」(テレビ出演), テレビ愛知,  - 2012 05 14 , 「頻尿と尿失禁」


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