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TSUCHIYA Syuhei

    Graduate School of Medical Sciences Department of Oral and Maxillofacial Surgery　Lecturer
Last Updated :2024/03/19

Researcher Information

J-Global ID

Research Interests

  • Osseointegration   Dental implant   Bone   

Research Areas

  • Life sciences / Regenerative dentistry and dental engineering

Academic & Professional Experience

  • 2021/04 - Today  Nagoya City UniversityDepartment of Oral and Maxillofacial Surgery, Nagoya City University Graduate School of Medical SciencesAssistant professor
  • 2020/01 - 2021/03  やまて歯科
  • 2011/01 - 2019/12  Nagoya University HospitalOral and Maxillofacial SurgeryAssistant Professor
  • 2009/09 - 2010/12  Nagoya University HospitalOral and Maxillofacial SurgeryMedical Staff
  • 2008/09 - 2009/08  ミシガン大学歯学部生体材料科学講座研究員
  • 2008/04 - 2008/08  Nihon UniversityAnatomy II兼任講師
  • 2004/04 - 2008/03  Nagoya UniversityGraduate School of Medicine大学院生
  • 2002/04 - 2004/03  Nagoya University HospitalOral and Maxillofacial SurgeryResident

Education

  • 2004/04 - 2008/03  名古屋大学大学院  医学系研究科
  • 1996/04 - 2002/03  Okayama University  歯学部

Association Memberships

  • 顎顔面インプラント学会   日本口腔科学会   JAPANESE SOCIETY OF ORAL IMPLANTOLOGY   JAPAN PROSTHODONTIC SOCIETY   JAPANESE SOCIETY OF ORAL AND MAXILLOFACIAL SURGEONS   

Published Papers

Books etc

  • The Osteoperiosteal Flap: A Simplified Approach to Alveolar Bone Reconstruction
    土屋 周平 (Joint work)Quintessence Publishing 2010
  • 科学と工業
    土屋 周平 (Joint work組織工学的手法を用いた歯の再生)大阪工研協会 2008
  • 歯の再生
    土屋 周平 (Joint workアグリゲーション法を用いた歯の再生)真興交易 2006

MISC

Research Grants & Projects

  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    Date (from‐to) : 2021/04 -2024/03 
    Author : 土屋 周平; 黒田 健介; 加藤 伸一郎; 本田 雅規; 渋谷 恭之
     
    二酸化チタン上の骨髄由来間葉系細胞（BMSCs）のO結合型糖鎖の機能をin vitroで解析をした。BMSCsにおけるO結合型糖鎖の糖転移酵素であるキシロシルトランスフェラーゼ（XylT）-1、-2をRNA干渉技術でノックダウンし、それぞれshXylT-1、shXylT-2とした。対照群はベクターのみを形質転換し、NTCとした。それぞれの細胞をシングルセルクローニングし、安定発現細胞株を作成した。これらの細胞を二酸化チタン上で培養をすると、shXylT-1およびshXylT-2はNTCと比較して石灰化能を有意に低下した。また、二酸化チタン上におけるshXylT-1およびshXylT-2の細胞増殖能は、NTCと比較して有意に低下した。さらに、二酸化チタン上のshXylT-1は、IDO、PGES2、AHR、PD-L1、IL-10など、免疫寛容能を促進する因子の遺伝子発現がNTCと比較して有意に上昇した。また、二酸化チタン上のshXylT-2は、PGES2、IDO、TSG6、IL-6の発現がNTCと比較して有意に低下することが明らかになった。さらに、shXylT-1およびshXylT-2の培養上清を回収し、THP-1から分化させたマクロファージに添加をすると、shXylT-1の培養上清は、組織修復型のM2マクロファージに分化し、shXylT-2の培養上清は炎症型のM1マクロファージに分化する傾向がみられた。さらに、二酸化チタン上で培養したshXylT-1およびshXylT-2をHL-60から分化した好中球と共培養をすると、shXylT-2はNTCおよびshXylT-1と比較して有意にアポトーシス細胞数が低下した。 以上の結果からXylT-1およびXylT-2は、二酸化チタン上のBMSCsの石灰化能および免疫寛容能に影響を与えることが明らかになった。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2019/04 -2024/03 
    Author : 日比 英晴; 黒田 健介; 興戸 正純; 岡部 一登; 酒井 陽; 藤尾 正人; 土屋 周平
     
    われわれは骨髄由来間葉系幹細胞培養上清を利用した組織再生に取り組み，培養細胞はさまざまな刺激を加えることで骨形成・血管新生に関連する成長因子が多く産生することがわかってきた．本年度の研究目的は伸展刺激により細胞培養上清の骨形成・血管新生能がどう影響されるかを明らかにすることであった．細胞種はヒト骨髄由来間葉系幹細胞およびヒト歯根膜由来線維芽細胞とし，伸展刺激は培養細胞伸展システムを用いて加え，任意の伸展率と周期を設定した．24 時間培養後の細胞および培養上清を回収し，骨形成・血管新生関連因子の発現を遺伝子，タンパクレベルで評価した．またヒト骨芽細胞・ヒト臍帯静脈内皮細胞を使用して上清の石灰化能・管腔形成能についても評価した．さらにマウスの頭蓋骨欠損モデルを用いてアテロコラーゲンスポンジを担体として上清を適用し一定期間後の骨形成・血管新生について組織学的，X線学的に評価した．その結果，いずれの細胞種でも伸展刺激により骨形成・血管新生関連遺伝子の発現が上昇し，関連因子の含有量も増加しており，骨形成・血管新生能は向上していた．頭蓋骨欠損モデルではいずれの細胞種でも伸展刺激により骨形成・血管新生が促進されることを確認した．伸展刺激を加えることで，培養上清の骨形成・血管新生能が向上し，その投与により骨形成・血管新生が促進されることが示された．劣悪な培養環境にさらされた細胞は，生きのびようと反応し生成した物質を含んだ細胞外小胞を放出することを利用して，組織再生に適した合目的な細胞外小胞を調製することができると考える．この細胞外小胞を組織欠損部に適用することにより，動員した内在性の幹・前駆細胞を集積させて組織を再生させる方法を検討することが可能であり，生体内に潜在する組織再生能力を特別な装置なしで高め，引き出すことができるはずであり，本課題の最終目標は十分に実現し得ると確信する．
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2018/04 -2021/03 
    Author : Kuroda Kensuke
     
    Metals / alloys, sintered ceramics, and polymers were picked up as implant base materials. The surface of the base material was hydrophilized using a new process developed by the authors for the purpose of preparing the base of the hierarchical structure to be built on the surface of the base material. We optimized the adsorbing conditions for multiple substances (ions, polypeptides, surfactants, and proteins) for stepwise or simultaneous approach on the surface-modified material and evaluated them. From an engineering point of view, the correlation between the amount of adsorption / desorption and adsorption / desorption rate of substances and the chemical surface properties of the material was clarified, and by evaluating both in vivo and in vitro, cells and bacteria can be evaluated. We comprehensively examined the findings on adhesion and proliferation and the findings on biological reactions.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2018/04 -2021/03 
    Author : FUJIO Masahito
     
    Human bone marrow-derived mesenchymal stem cells and human periodontal ligament-derived fibroblasts were cultured by applying cyclic stretch stimulation. The conditioned media was collected and the secretome were analyzed. Angiogenesis-related factors (VEGF, PDGF-AA) and osteogenic factors (BMP-2, BMP-4) were released more than when cultured without applying cyclic stretch stimulation. An increase in angiogeneis and osteogenesis was observed in vitro and in vivo. It was suggested that by changing the cell culture environment, a disease-specific conditioned medium could be obtained.
  • 日本学術振興会：基盤研究（C)
    Date (from‐to) : 2017/04 -2020/03 
    Author : 土屋 周平
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2016/04 -2019/03 
    Author : Hibi Hideharu; YAMAMOTO akihito; KATAGIRI wataru
     
    Stimulation of stem cells by hypoxia or mechanical stress promoted gene expression related to osteogenesis and angiogenesis and increased the amount of protein resulting from them in the cell-conditioned media. The application of the conditioned media to various tissue defect and inflammatory tissue injury models controled inflammation, repaired and regenerated the tissue. The mechanism was that localized macrophages were oppositely polarized and locally mobilized tissue forming cells constructed a vascular network and repaired or formed compatible tissue. We identified the growth factors and substrates contained in the conditioned media, clarified the optimal combination of them according to the target function, and showed that they actually repaired or regenerated the tissue in experimental animal models.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
    Date (from‐to) : 2015/04 -2018/03 
    Author : OKIDO Masazumi
     
    Surface modification of various implant materials was carried out in order to deepen the control of implant surface hierarchical structure. Nb, Ta, Ti-Nb-Ta-Zr and so on were implanted in the rat tibia for 2 weeks, and the osteoconductivity was evaluated from the amount of hard tissue formation on the surface. It is found that it can be arranged by water drop contact angle without depending on the kind of material. With the water drop contact angle of 65 degree as the boundary, osteoconductivity improved as more hydrophilic or hydrophobic. Protein adsorption was strongly dependent on surface hydrophilicity as well as osteoconductivity. On the other hand, it succeeded in making the surface hydrophilic by surface modification of various plastics. It was clarified that polyether ether ketone (PEEK) with super-hydrophilicity had high osteoconductivity.
  • オッセオインテグレーションを形成するタンパク質の解析
    日本学術振興会：基盤研究（C)
    Date (from‐to) : 2014/04 -2017/03 
    Author : 土屋 周平
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2013/04 -2017/03 
    Author : Kuroda Kensuke
     
    Manufacturing processing of “Protein-adsorbed Bio-implant” was constructed by the combination of engineering and biological techniques. Protein-adsorbed bio-implant did not have any surface coating layers, and the proteins, such as fibronectin, decorin, and albumin, adsorbed to implant surface directly. The protein-adsorbed bio-implants were subjected to in vivo testing using rats. The correlation between in vivo results and in vitro was examined. The kind of adsorbed protein to the bio-implants did not influence the osteoconductivity and the implants with any kinds of protein had high osteoconductivity. Protein adsorption rate to the implant surface at very early stage, which was affected by the hydrophilicity/hydrophobicity of implant surface, showed the strong correlation with the osteoconductivity.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
    Date (from‐to) : 2014/04 -2016/03 
    Author : Kuroda Kensuke; HIBI Hideharu; TSUCHIYA Shuhei
     
    We developed the formation technique for “Bioimplants”, integrating medical experiences into engineering techniques. It is important to produce the hydrophilic surface in order to adsorb the several kinds of protein on the metallic, ceramics, and polymer implants. In this research, we found hydro-techniques for the super-hydrophilicity of these implants and for keeping the super-hydrophilicity. Several kinds of protein were adsorbed on the super-hydrophilic implants surfaces (bioimplant). Important proteins were found for the improvement of the osteoconductivity by the implantation of the protein-adsorbed samples in rats’ tibia.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2013/04 -2016/03 
    Author : HIBI Hideharu; YAMAMOTO Akihito; KURODA Kensuke; KATAGIRI Wataru; HATTORI Hisashi; TSUCHIYA Shuhei
     
    The components of the conditioned media collected from bone marrow mesenchymal stem cells and dental pulp stem cells were detected and partially quantified. We showed that the conditioned media enhanced cell adhesion ability, cell migration ability, angiogenic potential, anti-inflammatory action and anti-apoptotic effect, and that the conditioned media from hypoxic-cultured cells further enhanced the cell migration ability and the angiogenic potential in vitro. We also showed that the conditioned media promoted vascular network formation and consequent bone regeneration, and that the conditioned media from hypoxic-cultured cells further promoted this process in vivo.
  • 培養細胞由来の細胞外基質を用いた放射線性骨髄炎の治療法の開発
    日本学術振興会：若手研究（B)
    Date (from‐to) : 2012/04 -2014/03 
    Author : 土屋 周平
  • 組織工学的手法によって再生した骨に含まれる骨シアロタンパクの糖鎖構造と機能解析
    日本学術振興会：若手研究（B)
    Date (from‐to) : 2010/04 -2012/03 
    Author : 土屋 周平

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