Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
Date (from‐to) : 2016/04 -2019/03
Author : OKADA atsushi
In order to establish a crystal phagocytosis model of macrophage (Mφ), Mφ nuclei were stained with DAPI, cytoplasm with Phalloidin-iFluor 555, COM crystals with Alexa-Fluor 488, and phagocytosis ability was measured by imaging cytometry using Incell Analyzer.
Human spot urine was collected and the related factors of urinary Mφ were measured, and univariate analysis showed high AUC value of IL-4 and IL1a between healthy persons and first-time stone formers. Multinomial logit model analysis among 3 groups of healthy persons, first-time and recurrent stone formers showed that the combination of IL4, IL10, IL1b, GM-CSF and urinary Mg had discriminatory power of more than 87%.