Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2016/04 -2020/03
Author : TAKANARI keisuke
In this project period, we collected 24 samples of human keloid, hypertrophic scar, normal scar and normal skin samples. With two-photon microscopy imaging, we obtained unfixed and unstained collagen fiber images from samples. Analysis of fiber orientation in the obtained images revealed that 1: keloid has more aligned orientation of fibers compared to the normal skin, 2: orientation and its variance are differ between the locations in the body, 3: thus fiber orientation and variance are affected by mechanical stimulation to the tissue. These phenomenon may partially explain the variety of keloid phenotypes seen in the clinical practice.