Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2012/04 -2015/03
Author : JOH Takashi; TANIDA Satoshi; HIGASHIYAMA Shigeki
Colon epithelial cells, many expressed in monocyte cells I was paying attention to the ARP 36-2 seen. When IL-1β stimulates colon epithelial cell line, TNFα concentration in the culture solution in over time increased but TPA, LPS, increase was observed. On the other hand, when the monocytic cell line TPA stimulation, TNFα concentration in the culture solution is increased, IL-1β, the stimulation with TPA, increase was observed. Further, when the deletion of the ADAM17 by KB-R7785, siRNA, colon epithelial cells, IL-1β in the monocyte cell, the concentration increase due to TPA stimulation during TNFαshedding, was significantly inhibited. Furthermore, even if lacking the ARP36-2 by siRNA, similarly concentration rise due to IL-1β, TPA stimulus during TNFαshedding has been significantly suppressed. ARP36, it seemed to be made in inflammatory bowel disease novel therapeutic target to control the TNFαshedding.