Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2016/04 -2019/03
Author : MORITOKI yoshinobu
"Development of mouse model for undescended testes". We have established robust undescended testes model by rats. However, rodent model for spermatogonial stem cell research has been performed using mainly mice, not rats. Therefore, as a preliminary experiment before running RNA-sequence for human testis, we decided to perform RNA-sequence for mouse testis. We established robust mouse undescended testis model, not by hormonal deprivation, by surgical approach. Using this model, we will next perform molecular analysis of atrophic process of undescended testis.
"Amplification of tiny volume of mRNA". We have developed robust mRNAs amplification method in the laboratory. Finally we have amplified as small as 20 copies per cell of mRNAs. We next tried to run RNA-sequence, but have not yet established stable protocol.