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村瀬 貴幸ムラセ タカユキ

所属部署医学研究科共同研究教育センター
職名准教授
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Last Updated :2020/05/18

研究者基本情報

学位

  • 医学博士, 名古屋市立大学

研究活動情報

論文

  • Immunohistochemistry for identification of CCND1, NSD2, and MAF gene rearrangements in plasma cell myeloma., Murase T, Ri M, Narita T, Fujii K, Masaki A, Iida S, Inagaki H, Cancer science, 110, (8) 2600 - 2606,   2019年08月, 査読有り
  • Plasma cell myeloma positive for t(14;20) with relapse in the central nervous system., Murase T, Inagaki A, Masaki A, Fujii K, Narita T, Ri M, Hanamura I, Iida S, Inagaki H, Journal of clinical and experimental hematopathology : JCEH,   2019年08月, 査読有り
  • A mutation analysis of the EGFR pathway genes, RAS, EGFR, PIK3CA, AKT1, and BRAF, and TP53 gene in thymic carcinoma and thymoma type A/B3., Sakane T, Murase T, Okuda K, Saida K, Masaki A, Yamada T, Saito Y, Nakanishi R, Inagaki H, Histopathology,   2019年06月, 査読有り
  • Thymic inflammatory pseudotumor with multilocular thymic cyst caused by immunoglobulin G4-related disease., Oda R, Okuda K, Murase T, Watanabe T, Sakane T, Tatematsu T, Yokota K, Haneda H, Nakanishi R, Thoracic cancer, 10, (1) 116 - 119,   2019年01月, 査読有り
  • Postoperative radiotherapy for T1/2N0M0 mucoepidermoid carcinoma positive for CRTC1/3-MAML2 fusions., Okumura Y, Murase T, Saida K, Fujii K, Takino H, Masaki A, Ijichi K, Shimozato K, Tada Y, Nibu KI, Inagaki H, Head & neck, 40, (12) 2565 - 2573,   2018年12月, 査読有り
  • CCR4 mutations associated with superior outcome of adult T-cell leukemia/lymphoma under mogamulizumab treatment., Sakamoto Y, Ishida T, Masaki A, Murase T, Yonekura K, Tashiro Y, Tokunaga M, Utsunomiya A, Ito A, Kusumoto S, Iida S, Ueda R, Inagaki H, Blood, 132, (7) 758 - 761,   2018年08月, 査読有り
  • Four immunohistochemical assays to measure the PD-L1 expression in malignant pleural mesothelioma., Watanabe T, Okuda K, Murase T, Moriyama S, Haneda H, Kawano O, Yokota K, Sakane T, Oda R, Inagaki H, Nakanishi R, Oncotarget, 9, (29) 20769 - 20780,   2018年04月, 査読有り
  • Mutation analysis of the EGFR pathway genes, EGFR, RAS, PIK3CA, BRAF, and AKT1, in salivary gland adenoid cystic carcinoma., Saida K, Murase T, Ito M, Fujii K, Takino H, Masaki A, Kawakita D, Ijichi K, Tada Y, Kusafuka K, Iida Y, Onitsuka T, Yatabe Y, Hanai N, Hasegawa Y, Shinomiya H, Nibu KI, Shimozato K, Inagaki H, Oncotarget, 9, (24) 17043 - 17055,   2018年03月, 査読有り
  • Achromobacter Infection Is Rare in Japanese Patients with Pulmonary B-cell Lymphoma., Aoyama S, Masaki A, Sakamoto Y, Takino H, Murase T, Ohshima K, Yoshino T, Kato S, Inagaki H, Internal medicine (Tokyo, Japan), 57, (6) 789 - 794,   2018年03月, 査読有り
  • Improved clonality detection in Hodgkin lymphoma using a semi-nested modification of the BIOMED-2 PCR assay for IGH and IGK rearrangements: A paraffin-embedded tissue study., Han S, Masaki A, Sakamoto Y, Takino H, Murase T, Iida S, Inagaki H, Pathology international, 68, (5) 287 - 293,   2018年03月, 査読有り
  • A comparative study of PD-L1 immunohistochemical assays with four reliable antibodies in thymic carcinoma., Sakane T, Murase T, Okuda K, Takino H, Masaki A, Oda R, Watanabe T, Kawano O, Haneda H, Moriyama S, Saito Y, Yamada T, Nakanishi R, Inagaki H, Oncotarget, 9, (6) 6993 - 7009,   2018年01月, 査読有り
  • MYB, MYBL1, MYBL2 and NFIB gene alterations and MYC overexpression in salivary gland adenoid cystic carcinoma, Kana Fujii, Takayuki Murase, Shintaro Beppu, Kosuke Saida, Hisashi Takino, Ayako Masaki, Kei Ijichi, Kimihide Kusafuka, Yoshiyuki Iida, Tetsuro Onitsuka, Yasushi Yatabe, Nobuhiro Hanai, Yasuhisa Hasegawa, Hiroshi Inagaki, HISTOPATHOLOGY, 71, (5) 823 - 834,   2017年11月, 査読有り, Aims: Adenoid cystic carcinoma (AdCC) is one of the most common salivary gland malignancies and the long-term prognosis is poor. In this study, we examined alterations of AdCC-associated genes, MYB, MYBL1, MYBL2 and NFIB, and their target molecules, including MYC. The results were correlated to clinicopathological profile of the patients. Methods and results: Using paraffin tumour sections from 33 cases of salivary gland AdCC, we performed a detailed fluorescence in-situ hybridization (FISH) analysis for gene splits and fusions of MYB, MYBL1, MYBL2 and NFIB. We found that 29 of 33 (88%) AdCC cases showed gene splits in either MYB, MYBL1 or NFIB. None of the cases showed an MYBL2 gene alteration. AdCCs were divided genetically into six gene groups, MYB-NFIB (n = 16), MYB-X (n = 4), MYBL1-NFIB (n = 2), MYBL1-X (n = 1), NFIB-X (n = 6) and gene-split-negative (n = 4). AdCC patients showing the MYB or MYBL1 gene splits were associated with microscopically positive surgical margins (P = 0.0148) and overexpression of MYC (P = 0.0164). MYC expression was detected in both ductal and myoepithelial tumour cells, and MYC overexpression was associated with shorter disease- free survival of the patients (P = 0.0268). Conclusions: The present study suggests that (1) nearly 90% of AdCCs may have gene alterations of either MYB, MYBL1 or NFIB, suggesting the diagnostic utility of the FISH assay, (2) MYB or MYBL1 gene splits may be associated with local aggressiveness of the tumours and overexpression of MYC, which is one of the oncogenic MYB/ MYBL1 targets and (3) MYC overexpression may be a risk factor for disease-free survival in AdCC.
  • Improved clonality detection in B-cell lymphoma using a semi-nested modification of the BIOMED-2 PCR assay for IGH rearrangement: A paraffin-embedded tissue study, Yuma Sakamoto, Ayako Masaki, Satsuki Aoyama, Shusen Han, Kosuke Saida, Kana Fujii, Hisashi Takino, Takayuki Murase, Shinsuke Iida, Hiroshi Inagaki, PATHOLOGY INTERNATIONAL, 67, (9) 453 - 460,   2017年09月, 査読有り, The BIOMED-2 PCR protocol for targeting the IGH gene is widely employed for detecting clonality in B-cell malignancies. Unfortunately, the detection of clonality with this method is not very sensitive when paraffin sections are used as a DNA source. To increase the sensitivity, we devised a semi-nested modification of a JH consensus primer. The clonality detection rates of three assays were compared: the standard BIOMED-2, BIOMED-2 assay followed by BIOMED-2 re-amplification, and BIOMED-2 assay followed by semi-nested BIOMED-2. We tested more than 100 cases using paraffin-embedded tissues of various B-cell lymphomas, and found that the clonality detection rates with the above three assays were 63.9%, 79.6%, and 88.0%, respectively. While BIOMED-2 re-amplification was significantly more sensitive than the standard BIOMED-2, the semi-nested BIOMED-2 was significantly more sensitive than both the standard BIOMED-2 and BIOMED-2 re-amplification. An increase in sensitivity was observed in all lymphoma subtypes examined. In conclusion, tumor clonality may be detected in nearly 90% of B-cell lymphoma cases with semi-nested BIOMED-2. This ancillary assay may be useful when the standard BIOMED-2 fails to detect clonality in histopathologically suspected B-cell lymphomas.
  • Expression of cancer/testis antigens in salivary gland carcinomas with reference to MAGE-A and NY-ESO-1 expression in adenoid cystic carcinoma, Shintaro Beppu, Yohei Ito, Kana Fujii, Kosuke Saida, Hisashi Takino, Ayako Masaki, Takayuki Murase, Kimihide Kusafuka, Yoshiyuki Iida, Tetsuro Onitsuka, Yasushi Yatabe, Nobuhiro Hanai, Yasuhisa Hasegawa, Kei Ijichi, Shingo Murakami, Hiroshi Inagaki, HISTOPATHOLOGY, 71, (2) 305 - 315,   2017年08月, 査読有り, AimsCancer/testis antigens (CTAs) are detected in cancer cells but not in healthy normal tissues, with the exception of gametogenic tissues. CTAs are highly immunogenic proteins, and thus represent ideal targets for cytotoxic T-lymphocyte-mediated specific immune therapy. The aim of this study was to screen CTA expression in various types of salivary gland carcinoma and to clarify clinicopathological significance of MAGE-A and NY-ESO-1 expression in adenoid cystic carcinomas (AdCCs) of the salivary gland, which is one of the most common salivary gland carcinomas, and usually has a fatal outcome. Methods and resultsWe used immunohistochemistry to examine the expression of four CTAs (MAGE-A, NY-ESO-1, CT7, and GAGE7) in various types of salivary gland carcinoma (n = 95). When carcinoma cases were divided into low-grade and intermediate/high-grade types, NY-ESO-1 and CT7 were expressed more frequently in intermediate/high-grade carcinomas. We then focused on MAGE-A and NY-ESO-1 expression in a large cohort of adenoid cystic carcinomas (AdCCs) (n = 46). MAGE-A and NY-ESO-1 were frequently expressed in AdCC; specifically, MAGE-A was expressed in >60% of the AdCC cases. MAGE-A expression and tumour site (minor salivary gland) were identified as independent risk factors for locoregional tumour recurrence. ConclusionsThese findings suggest that CTAs may be expressed in a variety of salivary gland carcinomas, especially in those with higher histological grades. In addition, MAGE-A, which is frequently expressed in AdCC cases, may be a useful prognostic factor for poorer locoregional recurrence-free survival.
  • Striated duct adenoma presenting with intra-tumoral hematoma and papillary thyroid carcinoma-like histology, Yohei Ito, Kana Fujii, Takayuki Murase, Kosuke Saida, Yoshihide Okumura, Hisashi Takino, Ayako Masaki, Shintaro Beppu, Daisuke Kawakita, Kei Ijichi, Hiroshi Inagaki, PATHOLOGY INTERNATIONAL, 67, (6) 316 - 321,   2017年06月, 査読有り, Striated duct adenoma of the salivary gland is a rare benign tumor characterized by unilayered duct epithelium and striations of the tumor cell membranes. To the best of our knowledge, only six cases have been reported in the literature. Here we report an additional case, which was complicated by an intra-tumoral hematoma on clinical presentation and by papillary thyroid carcinoma-like histology on intra-operative frozen section diagnosis. An asymptomatic 78-year-old male presented with a two-year-history of a painless tumor of the left parotid. An intra-tumoral hematoma, which is unusual for a salivary gland tumor, was suspected from results of pre-operative radiology. The patient then underwent a left parotidectomy. The intra-operative frozen section diagnosis indicated a benign tumor, although ectopic papillary thyroid carcinoma was raised as a differential diagnosis since the eosinophilic tumor cells occasionally possessed nuclear grooves and nuclear pseudo-inclusions. By precise histopathological examination using paraffin sections, the tumor was finally diagnosed as striated duct adenoma. This type of tumor has unique features of hypervascular stroma and papillary thyroid carcinoma-like nuclei. In our case, the former feature was associated with the intra-tumoral hematoma and the latter feature, with difficulty in frozen section tumor diagnosis.
  • Cellular-level characterization of B cells infiltrating pulmonary MALT lymphoma tissues, Keiichiro Fujii, Ken-ichiro Ishibashi, Junki Kato, Jushin Kan, Kana Fujii, Yohei Ito, Hisashi Takino, Ayako Masaki, Takayuki Murase, Hiroshi Inagaki, VIRCHOWS ARCHIV, 469, (5) 575 - 580,   2016年11月, 査読有り, Mucosa-associated lymphoid tissue (MALT) lymphoma mainly consists of three types of tumor B cells, small (centrocyte-like), scattered large transformed, and intraepithelial. However, it is difficult to differentiate tumor B cells from reactive B cells at the cellular level. We examined five cases of API2-MALT1 fusion-positive MALT lymphoma of the lung. A single paraffin section for each case was subjected to sequential retrieval of whole-slide imaging (WSI) data of hematoxylin and eosin (HE) staining, immunofluorescence staining for CD79a, and fluorescence in situ hybridization (FISH) for the MALT1 split. We counted the number of MALT1 split-positive or MALT1 split-negative cells among CD79a-positive cells. The MALT1 split was detected in 59, 46, and 76 % of small, large, and intraepithelial B cells, respectively. A review of the HE-WSI data showed that cytomorphological distinction between the MALT1 split-positive and MALT1 split-negative B cells was virtually impossible. None of CD79a-negative lymphoid cells, epithelial cells, and microvascular endothelial cells was positive for MALT1 splits. As API2-MALT1 fusion is an early and critical event in the lymphomatogenesis, our findings are best interpreted as that a considerable number of B cells, either small, large, or intraepithelial, are reactive cells and that it is difficult to distinguish cytomorphologically between tumor B cells and reactive B cells. These findings suggest that the tumor architecture may be the central factor for making a correct histopathological diagnosis of MALT lymphoma. The sequential WSI of HE staining, immunofluorescence staining, and FISH as described here is a useful tool for pathological analysis at the cellular level.
  • Quantitative PCR for HTLV-1 provirus in adult T-cell leukemia/lymphoma using paraffin tumor sections, Junki Kato, Ayako Masaki, Keiichiro Fujii, Hisashi Takino, Takayuki Murase, Kentaro Yonekura, Atae Utsunomiya, Takashi Ishida, Shinsuke Iida, Hiroshi Inagaki, PATHOLOGY INTERNATIONAL, 66, (11) 618 - 621,   2016年11月, 査読有り, Detection of HTLV-1 provirus using paraffin tumor sections may assist the diagnosis of adult T-cell leukemia/lymphoma (ATLL). For the detection, non-quantitative PCR assay has been reported, but its usefulness and limitations remain unclear. To our knowledge, quantitative PCR assay using paraffin tumor sections has not been reported. Using paraffin sections from ATLLs and non-ATLL T-cell lymphomas, we first performed non-quantitative PCR for HTLV-1 provirus. Next, we determined tumor ratios and carried out quantitative PCR to obtain provirus copy numbers. The results were analyzed with a simple regression model and a novel criterion, cut-off using 95 % rejection limits. Our quantitative PCR assay showed an excellent association between tumor ratios and the copy numbers (r = 0.89, P < 0.0001). The 95 % rejection limits provided a statistical basis for the range for the determination of HTLV-1 involvement. Its application suggested that results of non-quantitative PCR assay should be interpreted very carefully and that our quantitative PCR assay is useful to estimate the status of HTLV-1 involvement in the tumor cases. In conclusion, our quantitative PCR assay using paraffin tumor sections may be useful for the screening of ATLL cases, especially in HTLV-1 non-endemic areas where easy access to serological testing for HTLV-1 infection is limited.
  • Pelvic tumors with normal-appearing shapes of ovaries and uterus presenting as an emergency (Review), Atsushi Imai, Satoshi Ichigo, Hiroshi Takagi, Kazutoshi Matsunami, Sadayoshi Watanabe, Takayuki Murase, Tsuneko Ikeda, ONCOLOGY LETTERS, 4, (1) 10 - 14,   2012年07月, 査読有り, Abdominal pain with an associated pelvic mass is a common problem in everyday practice. Concerns about ectopic pregnancy, torsion of an enlarged ovary or malignancy usually dominate the diagnostic evaluation. On physical and imaging examination, when a palpable painful mass is present in the pelvis and the two ovaries and uterus are detected in their normal anatomical locations, the content and origin of the lesions may be significant in narrowing the pre-operative differential diagnosis. Thus, the emergent pelvic indications discussed in this review should be considered. The causes of acute abdominal pain are few in number and therefore an accurate diagnosis may be most frequently made at the time of exploratory laparotomy.
  • Early diagnosis of malignant-transformed ovarian mature cystic teratoma: fat-suppressed MRI findings, Hiroshi Takagi, Satoshi Ichigo, Takayuki Murase, Tsuneko Ikeda, Atsushi Imai, JOURNAL OF GYNECOLOGIC ONCOLOGY, 23, (2) 125 - 128,   2012年04月, 査読有り, The most common form of malignant transformation developing from a mature cystic teratoma is squamous cell carcinoma, representing 80% of malignant transformations, while adenocarcinoma accounts for approximately 5%. Because of this rarity, few reports exist of preoperative diagnosis of this tumor by magnetic resonance imaging, in particular with fat suppression techniques. Here, we report magnetic resonance imaging findings and clinical features of a 79-year-old woman with mucinous adenocarcinoma arising from a mature cystic teratoma (measuring 5 x 6 cm), classified as surgical stage IA. Because of the poor prognosis of malignant transformation, when mature cystic teratomas are detected (even smaller than 5 cm tumor size) in postmenopausal women, serum tumor marker carcinoembryonic antigen levels and fat-suppressed magnetic resonance imaging may be potential indicators of malignant transformation.
  • Transitional cell carcinoma of the ovary, Satoshi Ichigo, Hiroshi Takagi, Kazutoshi Matsunami, Takayuki Murase, Tsuneko Ikeda, Atsushi Imai, ONCOLOGY LETTERS, 3, (1) 3 - 6,   2012年01月, 査読有り, Transitional cell carcinoma (TCC) of the ovary is a rare recently recognized subtype of ovarian epithelial cancer. Ovarian TCC has a modest response to chemotherapy, and metastatic TCC from the renal pelvis results in mortality. The clinical presentation is indistinguishable from other types of ovarian carcinoma. Histopathological examination remains the first tool used in the diagnosis of these heterogeneous tumors and in the separation of closely related tumors. Since it is generally accepted that surgical resection is the primary therapeutic approach, and patient outcomes following chemotherapy are better than for other types of ovarian cancers, it is a reasonable concept to detect tumors when they are still confined within the ovaries. Thus, the aim of this review was to describe typical cases of primary TCC, and to review the medical literature for information on TCC management in order to determine appropriate diagnostic methods and therapy.
  • Critical role for a single leucine residue in leukemia induction by E2A-PBX1, Richard Bayly, Takayuki Murase, Brandy D. Hyndman, Rachel Savage, Salima Nurmohamed, Kim Munro, Richard Casselman, Steven P. Smith, David P. LeBrun, MOLECULAR AND CELLULAR BIOLOGY, 26, (17) 6442 - 6452,   2006年09月, 査読有り, In roughly 5% of cases of acute lymphoblastic leukemia, a chromosomal translocation leads to expression of the oncogenic protein E2A-PBX1. The N-terminal portion of E2A-PBX1, encoded by the E24 gene, is identical in sequence to the corresponding portion of the E proteins E12/E47 and includes transcriptional activation domains. The C terminus consists of most of the HOX interacting transcription factor PBX1, including its DNA-binding homeodomain. Structure-frinction correlative experiments have suggested that oncogenesis by E2A-PBXI requires an activation domain, called AD1, at the extreme N terminus. We recently demonstrated that a potentially helical portion of AD1 interacts directly with the transcriptional coactivator protein cyclic AMP response element-binding protein (CBP) and that this interaction is essential in the immortalization of primary bone marrow cells in tissue culture. Here we show that a conserved LXXLL motif within AD1 is required in the interaction between E2A-PBX1 and the KIX domain of CBP. We show by circular dichroism spectroscopy that the LXXLL-containing portion of AD1 undergoes a helical transition upon interacting with the KIX domain and that amino acid substitutions that prevent helix formation prevent both the KIX interaction and cell immortalization by E2A-PBX1. Perhaps most strikingly, substitution of a single, conserved leucine residue (1,20) within the LXXLL motif impairs leukemia induction in mice after transplantation with E2A-PBX1-expressing bone marrow. The KIX domain of CBP mediates well-characterized interactions with several transcription factors of relevance to leukemia induction. Circumstantial evidence suggests that the side chain of L20 might interact with a deep hydrophobic pocket in the KIX domain. Therefore, our results serve to identify a potential new drug target.
  • Expression of glial cell line-derived neurotrophic factor family members and their receptors in pancreatic cancers, Y Ito, Y Okada, M Sato, H Sawai, H Funahashi, T Murase, T Hayakawa, T Manabe, SURGERY, 138, (4) 788 - 794,   2005年10月, 査読有り, Background. The glial cell line-derived neurotrophic factor (GDNF) is a member of neurotrophic Polypeptide family, which promotes survival and rescue of various neural cells in the central and peripheral nerve systems. We previously reported that GDNF promotes tumor cell invasion in pancreatic cancer cell lines. The purpose of this study was to investigate GDNF family expression and the status of related receptors in actual cancer tissues, and assess correlations with clinicopathologic behavior. Methods. Immunohistochemical assessment of GDNF, neurturin, persephin, artemin, GDNF family receptor alpha-1 and alpha-2, and RET was performed for 51 cases of surgically resected pancreatic cancer. Results. In all intrapancreatic nerves, GDNF and artermin were expressed strongly. In pancreatic cancer tissues. The expression of RET was stronger than that seen in normal ductal cells and was significantly related to the survival rate after resection (P = .026) and lymphatic invasion (P = .014). Intrapancreatic neural invasion was significantly related to the expression of GDAT (P = .047). Conclusions. We conclude that the expression of RET in pancreatic cancer tissues may be a useful prognostic marker and GDNF may play an important role in neural invasion.
  • F-18FDG uptake in a malignant localized fibrous tumor of the pleura, M Hara, M Kume, H Oshima, N Shibamoto, A Iida, Y Mori, A Nakamura, T Murase, JOURNAL OF THORACIC IMAGING, 20, (2) 118 - 119,   2005年05月, 査読有り, In the case presented here, FDG-PET was performed to evaluate the possibility of malignancy High FDG accumulation, with a standardized uptake ratio (SUR) of 3.0, was noted in an upper nodular compartment of the mass that exhibited malignant features histopathologically. It was suggested that FDG-PET is helpful to know which parts of lesions are benign or malignant in patients with LFTP whose prognoses are usually difficult to predict.
  • False-positive and true-negative hilar and mediastinal lymph nodes on FDG-PET - Radiological-pathological correlation, N Shiraki, M Hara, H Ogino, Y Shibamoto, A Iida, T Tamaki, T Murase, T Eimoto, ANNALS OF NUCLEAR MEDICINE, 18, (1) 23 - 28,   2004年02月, 査読有り, Objective: To compare histological findings of FDG-PET false-positive and true-negative hilar and mediastinal lymph nodes. Methods: Sixty-seven lymphnode areas in I I patients who were diagnosed to have N3 lymph nodes by FDG-PET and underwent surgery were histologically examined, and the histopathological findings in false-positive and true-negative lymph nodes were compared. Lymph nodes with higher accumulation of FDG than the surrounding mediastinum level were judged as positive. On histological sections, proportions of macrophages and lymphocytes, amount of coal dust deposit, presence of silicotic nodules, long- and short-axes of the largest node, and volume of macrophages and lymphocytes were evaluated. Correlations between the above-mentioned factors and FDG accumulation were evaluated. Results: FDG uptake was not correlated with the proportion of macrophages and lymphocytes, coal dust amounts, or the presence of silicotic nodules. The long- and short-axes of the largest node in the false-positive areas were significantly longer than those in the true-negative areas (p = 0.01, and 0.001, respectively). Volumes of lymph nodes (mean +/- SD: 150 +/- 190 mm(3)) and macrophages (78 +/- 71 mm(3)) in false-positive areas were markedly larger than those in true-negative areas (68 +/- 87 mm(3), p = 0.0009 and 34 +/- 54 mm(3), p = 0.0001, respectively). The volume of lymphocytes was also larger in false-positive areas but less markedly. Conclusion: Our study suggested that false-positive results of FDG-PET in hilar and mediastinal lymph nodes were closely related to the size of lymph node and the volume of macrophages.
  • Clonality analysis of different histological components in combined small cell and non-small cell carcinoma of the lung, T Murase, H Takino, S Shimizu, H Inagaki, H Tateyama, E Takahashi, H Matsuda, T Eimoto, HUMAN PATHOLOGY, 34, (11) 1178 - 1184,   2003年11月, 査読有り, Combined small cell and non-small cell carcinoma is relatively rare in the lung. Examination of the clonal relationship of different components in this type of tumor may give a clue to the rarity. We retrieved 6 such tumors; all 6 had small cell carcinoma and adenocarcinoma components, and 3 had an additional squamous cell carcinoma component. We examined the point mutations in the p53 gene and allelic loss (ie, the loss of heterozygosity [LOH] pattern) of chromosome 3p in each component. p53 mutations were detected in the small cell carcinoma component of 5 tumors and in the non-small cell carcinoma components of 2 tumors. In I case, the squamous cell carcinoma component had a p53 mutation locus identical to that in the small cell carcinoma component, but in the other case, the adenocarcinoma component had a different mutation than that in the small cell carcinoma component. Chromosome 3p LOH loci in the squamous cell carcinoma component were present in the small cell carcinoma component in all 3 cases, but some LOH loci were not identical in the small cell carcinoma and adenocarcinoma components in 3 cases. These results suggest that the small cell and squamous cell carcinoma components of combined small cell lung carcinomas have an intimate clonal relationship. On the other hand, the adenocarcinoma component often may be derived from a separate clone or, more likely, undergo a progressive process separate from the squamous cell-small cell carcinoma beginning in a very early stage, that is, before the appearance of p53 and chromosome 3p abnormalities. This tumorigenesis process may explain the relative rarity of combined small cell and non-small cell carcinoma, which occurs primarily in the peripheral lung, an infrequent site of squamous cell carcinoma. (C) 2003 Elsevier Inc. All rights reserved.
  • Sclerosing hemangioma with metastases to multiple nodal stations, M Yano, Y Yamakawa, M Kiriyama, M Hara, T Murase, ANNALS OF THORACIC SURGERY, 73, (3) 981 - 983,   2002年03月, 査読有り, We present a case of a large pulmonary sclerosing hemangioma with metastases to multiple lymph nodal stations and suspected contralateral pulmonary metastasis. Four cases (including the present) have been reported to have lymph node metastasis, and all had large tumors exceeding 15 cm in diameter. Accordingly, resection of sclerosing hemangioma is advisable while the tumor is small. Even in cases with a large sclerosing hemangioma, lymph node metastasis may be uncommon. However lymph node dissection may be necessary to detect lymph node metastasis in selected cases. (C) 2002 by The Society of Thoracic Surgeons 10.
  • Nasal NK-cell lymphoma followed by relapse in the uterine cervix, T Murase, H Inagaki, N Takagi, M Okabe, T Eimoto, LEUKEMIA & LYMPHOMA, 43, (1) 203 - 206,   2002年01月, 査読有り, We report a case of nasal natural killer (NK)-cell lymphoma in a 51-year-old Japanese woman who showed a later relapse in the uterine cervix. The nasal NK-cell lymphoma regressed after local radiation therapy. Six months after the diagnosis while the patient was being treated with chemotherapy for a subclinical tumor, a mass lesion of the uterine cervix was noticed by follow-up computed tomography. Giemsa-stained vaginal smear showed lymphoid tumor cells with large azurophilic granules, leading to a rapid diagnosis of cervical involvement by NK-cell lymphoma. The chemotherapy regimens were immediately changed, but the patient died 2 months after the relapse with an overall survival of 8 months. This case may be of value in elucidating the biological behavior and natural history of NK-cell lymphoma.


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