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佐藤 剛サトウ タケシ

所属部署医学研究科産科婦人科学分野
職名准教授
メールアドレスog.satomed.nagoya-cu.ac.jp
ホームページURL
生年月日
Last Updated :2019/07/06

研究者基本情報

学歴

  •  - 1990年, 名古屋市立大学, 医学部

学位

  • 名古屋市立大学医学部/博士(医学)

所属学協会

  • 日本産科婦人科学会
  • 日本人類遺伝学会
  • 日本生殖医学会
  • 日本受精着床学会
  • 日本先天異常学会
  • 日本周産期・新生児医学会

研究活動情報

研究分野

  • 外科系臨床医学, 産婦人科学

研究キーワード

    着床前スクリーニング, 胚, 割球, 着床前診断, 不妊症

論文

  • Clinical utility of decorin in follicular fluid as a biomarker of oocyte potential, Yuki Sawada, Takeshi Sato, Chieko Saito, Fumiko Ozawa, Yasuhiko Ozaki, Mayumi Sugiura-Ogasawara, Reproductive Biology, 18, 33 - 39,   2018年03月, 査読有り, © 2017 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn This study investigated the concentration of decorin (DCN) in mature follicular fluid and the existence in the granulosa cells. It also investigated whether DCN is useful as a biomarker for outcomes of assisted reproductive technology (ART). A retrospective cohort study was performed involving 130 oocytes of 88 patients treated with ART because of unexplained infertility. The concentration of DCN in the follicular fluid (F-DCN) was 39.26 ng/ml (median value); it was higher than that in serum. F-DCN of the oocytes fertilized by intracytoplasmic sperm injection (ICSI) was significantly lower than that of oocytes that were not fertilized (33.24 ng/ml vs 40.18 ng/ml; P = 0.043). When a cut-off level of 34.5 ng/ml was set according to the receiver-operating characteristic curve, the fertilization rate of the oocytes from the follicles in which F-DCN was lower than the cut-off level tended to be good compared to that of the oocytes with F-DCN higher than the cut-off level (P = 0.052). DCN is less likely to be produced by the granulosa cells (GCs), because it was not detected in GCs by immunostaining and Western blot analysis. F-DCN has a possibility to be a biomarker indicating the quality of oocytes collected from the corresponding follicle.
  • Preimplantation genetic diagnosis and natural conception: A comparison of live birth rates in patients with recurrent pregnancy loss associated with translocation, Shinichiro Ikuma, Shinichiro Ikuma, Takeshi Sato, Mayumi Sugiura-Ogasawara, Motoi Nagayoshi, Atsushi Tanaka, Satoru Takeda, PLoS ONE, 10,   2015年06月, 査読有り, © 2015 Ikuma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Background: Established causes of recurrent pregnancy loss (RPL) include antiphospholipid syndrome, uterine anomalies, parental chromosomal abnormalities, particularly translocations, and abnormal embryonic karyotypes. The number of centers performing preimplantation genetic diagnosis (PGD) for patients with translocations has steadily increased worldwide. The live birth rate with PGD was reported to be 27-54%. The live birth rate with natural conception was reported to be 37-63% on the first trial and 65-83% cumulatively. To date, however, there has been no cohort study comparing age and the number of previous miscarriages in matched patients undergoing or not undergoing PGD. Thus, we compared the live birth rate of patients with RPL associated with a translocation undergoing PGD with that of patients who chose natural conception. Methods and Findings: After genetic counseling, 52 patients who desired natural conception and 37 patients who chose PGD were matched for age and number of previous miscarriages and these comprised the subjects of our study. PGD was performed by means of fluorescence in situ hybridization analysis. The live birth rates on the first PGD trial and the first natural pregnancy after ascertainment of the carrier status were 37.8% and 53.8%, respectively (odds ratio 0.52, 95% confidence interval 0.22-1.23). Cumulative live birth rates were 67.6% and 65.4%, respectively, in the groups undergoing and not undergoing PGD. The time required to become pregnancy was similar in both groups. PGD was found to reduce the miscarriage rate significantly. The prevalence of twin pregnancies was significantly higher in the PGD group. The cost of PGD was $7,956 U.S. per patient. Conclusions: While PGD significantly prevented further miscarriages, there was no difference in the live birth rate. Couples should be fully informed of the similarity in the live birth rate, the similarity in time to become pregnancy, the advantages of PGD, such as the reduction in the miscarriage rate, as well as its disadvantages, such as the higher cost, and the advantages of a natural pregnancy, such as the avoidance of IVF failure. The findings presented here should be incorporated into the genetic counseling of patients with RPL and carrying a translocation.
  • Comparison of follicular fluid and serum anti-Mullerian hormone levels as predictors of the outcome of assisted reproductive treatment, Yukio Hattori, Takeshi Sato, Hideki Okada, Chieko Saito, Mayumi Sugiura-Ogasawara, European Journal of Obstetrics Gynecology and Reproductive Biology, 169, 252 - 256,   2013年07月, 査読有り, Objective To compare follicular fluid and serum concentrations of anti-Mullerian hormone (AMH) as predictors of the outcome of assisted reproduction. Study design This observational study enrolled 58 women who were undergoing IVF or ICSI treatment with the long stimulation protocol. Patients diagnosed as having PCOS were excluded. Serum and follicular fluid AMH levels were assessed as predictors of clinical pregnancy. Results Both the serum and follicular fluid AMH levels were higher in the clinical pregnancy group than in the failed group. A significant correlation was found between the serum and follicular fluid AMH levels, but a discrepancy was observed in some patients with elevated AMH levels in the follicular fluid or serum alone. Assisted reproductive treatment resulted in clinical pregnancy in all of the patients with elevated AMH levels in the follicular fluid (>40 pM) or in the serum (>10 pM). The ROC-AUC for the combination of follicular and serum AMH was 0.772, which was relatively higher than that for either the serum AMH (AUC: 0.691) or follicular fluid AMH (AUC: 0.688) alone. Conclusion(s) Elevated AMH levels in either the serum or follicular fluid appeared to be predictive of clinical pregnancy, even if AMH levels in other fluids were low. This is a pilot study with preliminary data that need further confirmation. © 2013 Elsevier Ireland Ltd. All rights reserved.
  • 新生児および妊婦褥婦由来B群連鎖球菌の薬剤感受性と血清型−他施設共同研究 1999年〜2000年−., 脇本寛子, 脇本幸夫, 矢野久子, 松原康策, 宮川創平, 吉田 敦, 奥住捷子, 佐藤洋子, 澤田恭子, 山田恭聖, 二村真秀, 北川眞理子, 佐藤 剛, 南 正明, 長谷川忠男. , 感染症学雑誌, 85, 155 - 160 ,   2011年, 査読有り
  • 染色体相互転座に起因する習慣流産に対するFISHを用いた着床前診断の現況と技術的問題点., 佐藤 剛, 齋藤知恵子, 服部幸雄, 杉浦真弓., 20, 21 - 26,   2010年, 査読有り
  • The polycystic ovary syndrome does not predict further miscarriage in Japanese couples experiencing recurrent miscarriages, Mayumi Sugiura-Ogasawara, Takeshi Sato, Nobuhiro Suzumori, Tamao Kitaori, Kyoko Kumagai, Yasuhiko Ozaki, American Journal of Reproductive Immunology, 61, 62 - 67,   2009年01月, 査読有り, Problem: It has been a matter of controversy whether the polycystic ovary syndrome (PCOS) is actually a causal factor of miscarriages because of the absence of internationally established criteria. We, therefore, in this study investigated whether PCOS and a polycystic ovary (PCO) morphology have predictive value for subsequent miscarriages using new International and Japanese criteria. Method of study: A total of 195 patients with a history of two consecutive first trimester miscarriages and without abnormal chromosomes in either partner, antiphospholipid antibodies or uterine anomalies, were examined. The prospective pregnancy outcome was compared between patients with and without PCOS, PCO morphology, elevated luteinizing hormone (LH), hyperandrogenism and obesity. Results: Of a total of 195 patients, 56 (28.7%) miscarried subsequently. Three (1.5%) and 12 (6.2%) were diagnosed as suffering from PCOS by Japanese and International criteria respectively. There was no relation between a diagnosis of PCOS, PCO morphology, elevated LH, free testosterone or obesity and the subsequent miscarriage rate. Conclusions: A routine test for diagnosis of PCOS is not necessary in patients experiencing recurrent miscarriages because none of the related parameters examined in this study predicted subsequent miscarriage. © 2009 John Wiley & Sons A/S.
  • 着床後黄体機能不全に陥った自然周期の凍結融解胚移植例., 生田克夫, 永谷 都, 岡田英幹, 佐藤 剛, 服部幸雄, 杉浦真弓., 東海産科婦人科学会雑誌, 45, 65 - 70,   2009年, 査読有り
  • Occasional antiphospholipid antibody positive patients with recurrent pregnancy loss also merit aspirin therapy: A retrospective cohort-control study, Mayumi Sugiura-Ogasawara, Yasuhiko Ozaki, Tamao Nakanishi, Takeshi Sato, Nobuhiro Suzumori, Kyoko Kumagai, American Journal of Reproductive Immunology, 59, 235 - 241,   2008年03月, 査読有り, Problem: It is well known that treatment with aspirin plus heparin is effective for patients with antiphospholipid syndrome (APS) to prevent pregnancy loss. However, it is unclear if occasional antiphospholipid antibodies (aPL) are a risk factor and whether patients with aPL at one time point but not diagnosed as APS should be treated. Methods of study: We therefore studied whether aspirin alone is effective in patients with occasional aPL who did not meet the criteria for APS. We compared live birth rates between 52 patients with occasional aPL treated with aspirin and 672 unexplained patients with no medication. Patients in both group had a history of two or three pregnancy losses. Results: In all, 44 of 52 patients (84.6%) with occasional aPL could experience live birth when treated with aspirin alone. 509 of 672 patients (75.7%) with unexplained pregnancy losses could have babies. When miscarriage cases caused by an abnormal embryonic karyotype were excluded, the success rates were 95.7% (44/46) and 81.2% (509/621), respectively. The live birth rate in patients with occasional aPL treated with aspirin was significantly higher than that in unexplained patients with no medication (P = 0.008). Conclusion: We therefore conclude that aspirin is also useful in patients with occasional aPL but not APS. © Journal compilation © 2008 Blackwell Munksgaard.
  • Meiotic segregation analysis in male translocation carriers by using fluorescent in situ hybridization, N. Nishikawa, T. Sato, N. Suzumori, S. Sonta, K. Suzumori, K. Suzumori, International Journal of Andrology, 31, 60 - 66,   2008年02月, 査読有り, Balanced reciprocal and Robertsonian translocations are the most common structural chromosome abnormalities in humans, with incidences of 0.7 and 1.23 per 1000. These translocations can affect fertility and/or pregnancy outcome because of possibly impaired production of gametes with an unbalanced zygote caused by the parental arrangement. Fertility problems in male translocation carriers are because of various degrees of sperm alterations that are directly related to the disturbance of the meiotic process. Investigation of human sperm chromosomes was performed by karyotyping spermatozoa after penetration of zona-free hamster oocytes, karyotype analysis now being possible to analyse the segregation patterns by using fluorescent in situ hybridization (FISH). Here, we document the results of meiotic segregation analysis for four Robertsonian and four reciprocal translocation carriers by FISH. In the sperm of Robertsonian translocation males, the majority of spermatozoa were normal/balanced. On the other hand, males with reciprocal translocations demonstrated a high rate of unbalanced spermatozoa of about 50% on meiotic segregation, with an unusually high rate (23.5%) of 3 : 1 segregation. This knowledge can be used for genetic counselling of families with these types of translocations. © 2007 The Authors.
  • Successful pregnancy after artificial insemination in a case of human seminal plasma allergy, Aiko Makino, Takeshi Sato, Yukio Hattori, Chieko Saito, Mayumi Sugiura-Ogasawara, Chiyo Saito, Yoichi Shintani, Reproductive Medicine and Biology, 7, 119 - 122,   2008年01月, 査読有り, Human seminal plasma allergy in women is uncommon, but causes a variety of serious reactions, including urticaria, dyspnea and vomiting, in those that are affected. Semen barriers, such as condoms, are the most widely advocated method for avoiding these reactions. However, this is not acceptable to couples who wish to have children. We present a case of a woman with human seminal plasma allergy who became pregnant after the eighth cycle of artificial insemination using washed sperm from her spouse. © Journal compilation © 2008 Japan Society for Reproductive Medicine.
  • Uterine cervical inflammatory cytokines, interleukin-6 and -8, as predictors of miscarriage in recurrent cases, Yukio Hattori, Tamao Nakanishi, Yasuhiko Ozaki, Kyoko Nozawa, Takeshi Sato, Mayumi Sugiura-Ogasawara, American Journal of Reproductive Immunology, 58, 350 - 357,   2007年10月, 査読有り, Problem: Inflammatory changes frequently occur in cases of second trimester miscarriage or pre-term delivery, but little attention has been paid to this association with recurrent miscarriage. As interleukin-6 and interleukin-8 are inflammatory cytokines reported to be associated with bacterial vaginosis, intrauterine infections, and pre-term delivery, we here investigated whether they might have predictive value for spontaneous abortion in recurrent cases. Method of Study: Cervical mucus and sera were collected at 4-5 weeks' gestation from a total of 59 patients with a history of two or more unexplained consecutive first trimester miscarriages, and examined by enzyme-linked immunosorbent assay. Patients then were followed up without medication and their pregnancy outcomes were compared with the test results. Results: Of a total of 59 patients, 13 (22%) miscarried subsequently. Both IL-6 and IL-8 in cervical mucus were significantly higher in patients who miscarried subsequently than in those who had a live birth. In addition, there was no correlation between cervical mucus and serum concentrations of IL-6 and IL-8 take at the same time, and there was no relation with serum IL-6 and IL-8 levels between the two groups. Conclusion: Cervical IL-6 and IL-8 might have predictive value for cases of recurrent miscarriage. © 2007 The Authors Journal compilation © 2007 Blackwell Munksgaard.
  • 当院における精巣内精子回収法(TESE)または精巣上体精子吸引術(MESA)により得られた精子を用いた卵細胞質内精子注入法(ICSI)の臨床成績, 牧野亜衣子, 佐藤 剛, 服部幸雄, 杉浦真弓, 金子朋功, 佐々木昌一, 東海産科婦人科学会雑誌 , 43, (67) ,   2007年, 査読有り
  • 体外受精・胚移植治療におけるLaser assisted hatchingの効果の検討, 服部幸雄, 佐藤 剛, 齋藤知恵子, 牧野亜衣子, 杉浦真弓, 日本受精着床学会雑誌, 23/1,192-196, 192 - 196,   2006年, 査読有り
  • Elevated aldosterone in amniotic fluid and maternal blood has diagnostic potential in pregnancies complicated with a fetus of bartter syndrome, Tamao Nakanishi, Nobuhiro Suzumori, Nobuhiro Suzumori, Haruo Mizuno, Kazutaka Suzuki, Takeshi Sato, Mitsuyo Tanemura, Yoshikatsu Suzuki, Kaoru Suzumori, Fetal Diagnosis and Therapy, 20, 481 - 484,   2005年11月, 査読有り, Pregnancies with fetuses affected with the Bartter syndrome, an autosomal recessive disorder of hyperreninism and hyperaldosteronism, are complicated by early onset of polyhydramnios which results in preterm deliveries. We have assessed biochemical changes in amniotic fluid and the mother's blood with a view to early diagnosis. Aldosterone levels of both amniotic fluid and the mother's blood were found to be increased at 27 weeks of gestation, while electrolyte levels did not differ significantly from those reported earlier for controls. After birth the baby suffered from polyuria with hyponatremia, hypomagnesemia and hypercalciuria which could be controlled by treatment with sodium chloride and magnesium. Elevated aldosterone thus might be a useful marker for early diagnostic purposes. Copyright © 2005 S. Karger AG.
  • Uterine lavage in prenatal screening for aneuploidy in continuing pregnancies [1], Xiao Xi Zhao, Kaoru Suzumori, Nobuhiro Suzumori, Takeshi Sato, Yasuhiko Ozaki, Prenatal Diagnosis, 25, 960 - 962,   2005年10月, 査読有り
  • 出生前診断された胎児心疾患33例の検討, 野沢恭子, 鈴森伸宏, 服部幸雄, 中西珠央, 山本珠生, 金子さおり, 佐藤 剛, 種村光代, 山口幸子, 水野寛太郎, 鈴木佳克, 杉浦真弓, 東海産科婦人科学会雑誌 , 42, 125 - 129,   2005年, 査読有り
  • 当院における非免疫性胎児水腫25例の検討, 中西珠央, 鈴森伸宏, 山本珠生, 金子さおり, 佐藤 剛, 種村光代, 福田純男, 鈴木佳克, 杉浦真弓, 東海産科婦人科学会雑誌 , 42, 145 - 150,   2005年, 査読有り
  • 膀胱内精子を用いた顕微授精により妊娠成立した逆行性射精の一例, 牧野亜衣子, 佐藤 剛, 服部幸雄, 杉浦真弓, 佐々木昌一, 鈴森 薫., 東海産科婦人科学会雑誌 , 42, 109 - 112,   2005年, 査読有り
  • Role of apoptosis controlled by cytochrome c released from mitochondria for luteal function in human granulosa cells., 53/2,   2005年, 査読有り
  • 体外受精胚移植治療における採卵時卵巣血流動態, 佐藤 剛, 生田克夫, 牧野亜衣子, 岡田英幹, 鈴森 薫, 産婦人科の実際, 53, 615 - 620,   2004年, 査読有り
  • Poor prognosis of recurrent aborters with either maternal or paternal reciprocal translocations., Sugiura-Ogasaware M, Ozaki Y, Sato T, Suzumori N, Suzumor K., Fertil. Steril., 81, 367 - 373,   2004年, 査読有り
  • The use of aspirin and low molecular weight ofheparin to prevent from the recurrence of maternal floor infarction in women without evidence of antiphospholipid antibody syndrome., Makino A, Suzuki Y, Yamamoto T, Sato T, Kojima K, Suzumori K, Fetal Diag Ther, 19, 261 - 265,   2004年, 査読有り
  • Examination of fetal cells and cell-free fetal DNA in maternal blood for fetal gender determination., Zhao XX, Suzumori N, Ozaki Y, Sato T, Suzumori K, Gynecol Obstet Invest, 58, 57 - 60,   2004年, 査読有り
  • A case of postpartum cardiomyopathy and pulmonary edema due to left ventricular systolic dysfunction., Suzumori N, Suzuki Y, Okada J, Nakanishi T, Sato T, Sugiyama M, Ohte N, Suzumori K, Acta Obstet Gynecol Scand, 83, 507 - 509,   2004年, 査読有り
  • Characteristic findings for diagnosis of baby complicated with both the VACTERL association and duodenal atresia., Fujishiro E, Suzuki Y, Sato T, Kondo S, Miyachi M, Suzumori K., Fetal Diag Ther , 19, 134 - 137,   2004年, 査読有り
  • Reduction of pleural effusion by OK-432 in a fetus complicated with congenital hydrothorax., Tsukihara A, Tanemura M, Suzuki Y, Sato T, Tanaka T, Suzumori K., Fetal Diag Ther , 19, 327 - 331,   2004年, 査読有り
  • Prenatal findings for complete trisomy 9 [5], Nobuhiro Suzumori, Takeshi Sato, Junko Okada, Tamao Nakanishi, Kenji Shirai, Mitsuyo Tanemura, Yoshikatsu Suzuki, Kaoru Suzumori, Prenatal Diagnosis, 23, 866 - 868,   2003年10月, 査読有り
  • 体外受精・胚移植治療におけるL-arginine併用療法の効果の検討, 生田克夫, 李 世隆, 牧野亜衣子, 青山和史, 佐藤 剛, 松原寛和, 岡田英幹, 鈴森 薫, 産婦人科の実際, 52, 361 - 367,   2003年, 査読有り
  • Comparison between fluorescence in situ hybridization (FISH) and quantitative fluorescent polymerase chain reaction (QF-PCR) for the detection of aneuploidies in single blastomeres., 佐藤 剛, 名古屋市立大学, 23/,678-84,   2003年, 査読有り
  • Conservative treatment by angiographic artery embolization of an 12 week cervical pregnancy following a period of heavy bleeding., Suzumori N, Katano K, Sato T, Okada J, Nakanishi T, Muto D, Suzuki Y, Ikuta K, Suzumori K. , Fertil Steril, 80, 617 - 619,   2003年, 査読有り
  • Prenatal diagnosis of triple X using fetal cells obtained by endocervical lavage., Xiao XZ, Suzumori K, Sato T., Prenta Diagn, 23, 549 - 551,   2003年, 査読有り
  • Cardiac failure caused by severe pre-eclampsia with placental abruption, and its treatment with anti-hypertensive drugs., Aoyama K, Suzuki Y, Sato T, Yamamoto T, Kojima K, Usami T, Ohte N, Suzumori K, J Obstet Gynaecol Res , 29, 339 - 342,   2003年, 査読有り
  • Prenatal diagnosis of lissencephaly (type II) by ultrasound and fast magnetic resonance imaging, Kazuhisa Kojima, Kazuhisa Kojima, Yoshikatsu Suzuki, Koichiro Seki, Tamao Yamamoto, Takeshi Sato, Taihei Tanaka, Kaoru Suzumori, Fetal Diagnosis and Therapy, 17, 34 - 36,   2002年02月, 査読有り, We report a case of lissencephaly which could be diagnosed by detailed examination during pregnancy. We first found bilateral enlarged ventricles in the fetus by routine abdominal ultrasonography at mid-pregnancy. Fast scanning MRI subsequently allowed confirmation of a diagnosis of lissencephaly during pregnancy. Copyright © 2002 S. Karger AG, Basel.
  • クエン酸クロミフェンDay1投与の検討, 日本受精着床学会雑誌, 19/6,   2002年, 査読有り
  • Enrichment of fetal cells from maternal blood by magnetic activated cell sorting (MACS) with fetal cell specific antibodies: one-step versus two-step MACS., Xaio XZ, Ozaki Y, Suzumori N, Sato T, Suzumori K., Congenital Anomalies , 42, 120 - 124,   2002年, 査読有り
  • An examination of different fetal specific antibodies and magnetic activated cell sorting for the enrichment of fetal erythroblasts from maternal blood., Xiao XZ, Ozaki Y, Suzumori N, Sato T, Suzumori K. , Congenital Anomalies , 42, 175 - 180,   2002年, 査読有り
  • Pneumomediastinum secondary to hyperemesis gravidarum during early pregnancy, T. Yamamoto, Y. Suzuki, K. Kojima, T. Sato, M. Tanemura, M. Kaji, Y. Yamakawa, M. Yokoi, K. Suzumori, Acta Obstetricia et Gynecologica Scandinavica, 80, 1143 - 1145,   2001年12月, 査読有り
  • クエン酸クロミフェンDay1投与の検討, 青山和史, 佐藤 剛, 松原寛和, 生田克夫, 鈴森 薫, 日本受精着床学会雑誌, 19, 34 - 37,   2001年, 査読有り
  • 初期胚の染色体異常の検討, 佐藤 剛, 青山和史, 鈴木由佳, 松原寛和, 生田克夫, 鈴森 薫, 日本受精着床学会雑誌, 18/,34-37, 34 - 37,   2001年, 査読有り
  • Gonadotropins and cytokines affect luteal function through control of apoptosis in human luteinized granulosa cells, Hirokazu Matsubara, Hirokazu Matsubara, Katsuo Ikuta, Yasuhiko Ozaki, Yuka Suzuki, Noritaka Suzuki, Takeshi Sato, Kaoru Suzumori, Journal of Clinical Endocrinology and Metabolism, 85, 1620 - 1626,   2000年01月, 査読有り, The luteal phase in the normal human menstrual cycle is known to be about 14 days. The physiological mechanisms that regulate the corpus luteum remain to be clarified, although apoptosis is reported to be involved. This study was undertaken to investigate the regulation of luteal function by gonadotropins, cytokines, and PGs, concentrating attention on the incidence of apoptosis and its molecular mechanisms in cultured human luteinized granulosa cells collected at oocyte pick-up from patients undergoing in vitro fertilization and embryo transfer. Clusters of granulosa cells were pipetted in 0.1% hyaluronidase in phosphate-buffered saline. After cell separation by centrifugation using Ficoll-Paque, 1 × 104viable cells/mL in RPMI 1640 medium with 10% FCS were used for experimentation. Substances added were FSH (100 ng/mL), hCG (100 ng/mL), LH (100 ng/mL), interleukin-1β (IL-1β; 10 ng/mL), transforming growth factor-β1 (TGFβ1; 10 ng/mL), macrophage colony-stimulating factor (M-CSF; 10 ng/mL), tumor necrosis factor-α (TNFα; 10 ng/mL), and PGF2α(10 ng/mL). After 24-h culture at 37 C under 5% CO2and air, cells were fixed with 4% neutral buffered formalin and stained with Hoechst 33258. Apoptotic bodies were counted under a fluorescence microscope, and immunostaining was performed using anti-Fas, Fas ligand, Bcl-2, Bax, and p53 antibodies. Incidences of apoptotic bodies in the group without substance addition were 0.7 ± 0.2% (0 h), 5.9 ± 0.6% (24 h), and 7.9 ± 1.2% (48 h); spontaneous increase was significant at the latter time points. Defining the incidence at 24 h as 100%, values after treatment were: FSH, 57%; LH, 84%; hCG, 44%; IL-1β, 76%; TGFβ1, 52%; M-CSF, 50%; TNFα, 177%; and PGF2α, 147%. Significant suppression was observed with FSH, hCG, TGFβ1, and M-CSF (P < 0.01). On the other hand, significant induction occurred with TNFα and PGF2α(P < 0.01). On immunostaining, the incidence of stained cells with anti-Fas, Fas ligand, Bax, and p53 antibody was increased after 24-h incubation without addition. This was reduced by hCG, TGFβ1, and M-CSF. No stained cells were observed with anti-Bcl-2 antibody before or after incubation. In conclusion, our results suggest that both gonadotropins (FSH and hCG) and cytokines (TGFβ1 and M-CSF) may be involved in the support of luteal function via suppression of apoptosis, and that TNFα and PGF2αmay contribute to ovarian dysfunction and/or luteal regression via its induction in human luteinized granulosa cells. Our results also suggest that Fas, Fas ligand, p53, and Bax may play roles in this apoptosis controlled by hCG, TGFβ1, and M-CSF.
  • 初期胚の形態と染色体異常, 産婦人科の世界, 52/11,983-992,   2000年, 査読有り
  • 着床前診断, 東海産科婦人科学会雑誌, 37/,25-33,   2000年, 査読有り
  • 体外受精胚移植において低採卵率を示した周期の内分泌学的背景の検討, 鈴木由佳, 青山和史, 鈴木規敬, 佐藤 剛, 松原寛和, 生田克夫, 鈴森 薫, 日本受精着床学会雑誌, 17/2, 163 - 167,   2000年, 査読有り
  • 体外受精・胚移植治療余剰胚における染色体異常の検索, 佐藤 剛, 青山和史, 鈴木由佳, 鈴木規敬, 松原寛和, 生田克夫, 鈴森 薫, 東海産科婦人科学会雑誌, 36/,29-33, 73 - 76,   1999年, 査読有り
  • 体外受精胚移植における子宮動脈血流PI値の測定, 中由佳, 浅野順子, 鈴木規敬, 佐藤 剛, 松原寛和, 生田克夫, 鈴森 薫, 東海産科婦人科雑誌, 35/2, 73 - 76,   1998年, 査読有り

MISC

  • スペシャリストに聞く 産婦人科でのアレルギー対応法 【疾患各論】精液アレルギー, 澤田祐季, 齋藤知恵子, 松川 泰, 佐藤 剛, 杉浦真弓, 臨床婦人科産科, 72,   2018年
  • 特集「生殖医療−知っておきたい最新トピックス」6. 着床前スクリーニング (PGS), 佐藤 剛, 産科と婦人科, 85,   2018年
  • 不育症のエビデンス〜命の大切さについて考える〜, 杉浦真弓, 佐藤 剛, 尾崎康彦, 思春期学, 34,   2016年
  • 着床前診断、着床前スクリーニングの実際, 佐藤 剛, 東海産科婦人科学会雑誌 , 53,   2016年
  • 特集① 話題の生殖医療 着床前診断、着床前スクリーニング, 佐藤 剛, 現代医学, 64,   2016年
  • 着床前診断、着床前スクリーニングの現状, 佐藤 剛, 東海産科婦人科学会雑誌 , 52,   2015年
  • 出生前診断を考える「着床前診断の現状と問題点」, 杉浦真弓, 佐藤 剛, 尾崎康彦, 日本医師会雑誌, 143,   2014年
  • 現代生殖医療のメインストリーム –ARTマスターたちの治療戦略- 「Ⅱ不妊の原因 8.不育症・習慣流産」., 杉浦真弓, 尾崎康彦, 片野衣江, 佐藤 剛, 北折珠央, 後藤志信, 産婦人科の実際, 63,   2014年
  • 産婦人科における臨床遺伝学 –ゲノム医療の展開:各論-「2. 着床前診断」, 佐藤 剛, 日本産婦人科医会 研修ノート, 92,   2014年
  • 生まれてくる子どもたちにとってよりよい生殖補助医療を, 佐藤 剛, エコ・あいち・マガジン, 5,   2013年
  • 生殖医療と周辺領域との関わり「I. 周産期 5. 着床前診断」, 佐藤 剛, 齋藤知恵子, 服部幸雄, 杉浦真弓, 産科と婦人科, 78,   2011年
  • 着床前診断の実践と問題点「トリプレットリピート病に対する着床前診断」, 佐藤 剛, 齋藤知恵子, 服部幸雄, 岡田英幹, 杉浦真弓, 産婦人科の実際, 58,   2007年
  • 着床前診断の実践と問題点「トリプレットリピート病に対する着床前診断」, 佐藤 剛, 杉浦真弓, 周産期医学36増刊 周産期医学必修知識第6版, 36,   2006年
  • 着床前診断の実践と問題点「トリプレットリピート病に対する着床前診断」, 佐藤 剛, 生田克夫, 青山和史, 鈴木由佳, 松原寛和, 鈴森 薫, 東海産科婦人科学会雑誌 , 37,   2000年
  • 不育症 臨床編 不育症と着床前診断, 杉浦真弓, 佐藤 剛, 服部幸雄, HORMONE FRONTIER IN GYNECOLOGY, 19

書籍等出版物

  • FISH法による胚の遺伝子診断法, Medical View社,   2000年
  • ARTラボラトリー 不妊治療の新しい展開のために, 佐藤 剛, 鈴森 薫, 分担執筆, FISH法による胚の遺伝子診断法, MEDICAL VIEW,   2000年

講演・口頭発表等

  • シンポジウム1 「不育症のトピックス」S1-3. 不育症に対するPGT-SR, PGT-Aの有効性., 佐藤 剛, 第63回日本生殖医学会学術講演会,   2018年09月06日, 招待あり
  • Clinical utility of decorin in follicular fluid as a biomarker of oocyte potential., 34th Annual Meeting of European Society of Human Reproduction and Embryology,   2018年07月01日
  • 特別講演Ⅰ「着床前診断、着床前スクリーニングの現況と展望」., 佐藤 剛, 第12回東海ARTカンファレンス,   2016年02月28日, 招待あり
  • 専攻医教育プログラム「着床前診断、着床前スクリーニングの実際」, 佐藤 剛, 第136回東海産科婦人科学会,   2016年02月13日, 招待あり
  • シンポジウム5 「着床前診断の現状と課題」「着床前胚における染色体異常解析の生児獲得率改善への効果」, 佐藤 剛, 日本人類遺伝学会第60回大会,   2015年10月14日, 招待あり
  • Preimplantation Genetic Diagnosis and Natural Conception: A Comparison of Live Birth Rates in Patients with Recurrent Pregnancy Loss Associated with Translocation., 1st Annual Meeting of European Society of Human Reproduction and Embryology,   2015年06月14日
  • Successful pregnancy after artificial insemination with washed sperm in a case of human seminal plasma allergy. , IFFS / JSRM International Meeting,   2015年04月26日
  • Time-lapse monitoring of oocytes activated artificially with electrical stimulation following intracytoplasmic sperm injection (ICSI) with immotile sperm retrieved by micro-dissection testicular sperm extraction (MD-TESE)., IFFS / JSRM International Meeting,   2015年04月26日
  • Conservative therapy with Gonadotropin-Releasing Hormone Agonist on uterine arteriovenous malformation in a patient with congenital heart disease., 39th Nordic Congress of Obstetrics and Gynecology,   2014年06月11日
  • 体外受精・胚移植治療における多前核卵由来の割球の染色体構成に関する検討., 佐藤 剛, 服部幸雄, 杉浦真弓, 第56回日本生殖医学会学術講演会,   2011年10月14日
  • シンポジウム2「PGDの現状と将来展望」2.単一遺伝子疾患のPGD 多様性と新たなアプローチ 1) トリプレットリピート病, 佐藤 剛, 齋藤知恵子, 服部幸雄, 杉浦真弓., 第29回日本受精着床学会,   2011年09月09日, 招待あり
  • 染色体均衡型相互転座を有する習慣流産患者の配偶子形成時における染色体分離様式の検討, 佐藤 剛, 服部幸雄, 杉浦真弓, 第63回日本産科婦人科学会学術講演会,   2011年08月11日
  • シンポジウム4「生殖医療とフローサイトメトリー」染色体相互転座に起因する習慣流産に対するFISHを用いた着床前診断の現況と技術的問題点., 佐藤 剛, 齋藤知恵子, 服部幸雄, 岡田英幹, 杉浦真弓, 第19回日本サイトメトリー学会学術集会,   2009年06月20日, 招待あり
  • 着床前診断を施行した筋強直性ジストロフィーの2例, 佐藤 剛, 服部幸雄, 杉浦真弓, 第60回日本産科婦人科学会学術講演会,   2008年04月12日
  • シンポジウム6「PGD(受精卵着床前診断)2007」筋強直性ジストロフィーの着床前診断の実際, 佐藤 剛, 杉浦真弓, 第25回日本受精着床学会学術講演会,   2007年10月26日, 招待あり
  • Plenary Session " Preimplantation Genetic Diagnosis of Chromosome Abnormalities in the Embryo using FISH and QF-PCR.", 20th International Congress of The Society of The Fetus as a Patient,   2004年04月23日
  • 卵細胞質内精子注入法(ICSI)における電気刺激法の有用性., 佐藤 剛, 牧野亜衣子, 青山和史, 岡田英幹, 生田克夫, 鈴森 薫, 第55回日本産科婦人科学会学術講演会,   2003年04月12日
  • Usefulness of adjuvant L-arginine treatment in in vitro fertilization and embryo transfer., 17th World Congress on Fertility and Sterility,   2001年11月26日
  • シンポジウム「ARTにおける最近の話題」初期胚の染色体異常の検討, 佐藤 剛, 第18回日本受精着床学会,   2000年07月06日, 招待あり
  • Detection of numerical aberrations of chromosomes 13, 18, 21, X and Y in single blastomres using QF-PCR and FISH. , 10th International Conference on Prenatal Diagnosis and Therapy,   2000年06月19日

競争的資金

  • 胚盤胞液内のDNAを用いた無侵襲性着床前診断技術の確立と臨床への応用, 文部科学省科学研究費 基盤研究 (C),   2015年04月 - 2018年03月, 佐藤 剛
  • 糖代謝異常の生殖内分泌、妊孕性への影響, 財団法人 愛知健康増進財団医学研究・健康増進活動等助成金,   2010年04月 - 2011年03月, 佐藤 剛
  • 胚盤胞期胚での筋強直性ジストロフィーに対する着床前診断に関する研究, 文部科学省科学研究費 基盤研究 (C) ,   2007年04月 - 2009年03月, 佐藤 剛
  • 筋強直性ジストロフィーに対する胚盤胞期胚での着床前診断の診断精度・診断効率に関する研究, 名古屋市立大学特別研究奨励費,   2007年04月 - 2009年03月, 佐藤 剛
  • ヒト胚盤胞細胞に対する分子生物学的手法を用いた着床前診断に関する研究, 文部科学省科学研究費 若手研究(B) ,   2013年04月 - 2005年03月, 佐藤 剛
  • ヒト胚盤胞細胞に対して分子生物学的手法を用いた着床前診断の研究, 名古屋市立大学学術振興会 学術研究奨励事業助成金,   2002年04月 - 2004年03月, 佐藤 剛
  • ヒト胚盤胞細胞に対する分子生物学的手法を用いた着床前診断に関する研究,   2003年 - 2004年


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