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河合 憲康カワイ ノリヤス

所属部署医学研究科腎・泌尿器科学分野
職名准教授
メールアドレス
ホームページURLhttp://www.med.nagoya-cu.ac.jp/index.Jhtm.
生年月日
Last Updated :2020/06/03

研究者基本情報

学歴

  •  - 1991年, 名古屋市立大学, 医学部

学位

  • 名古屋市立大学医学部/博士(医学), 名古屋市立大学

所属学協会

  • 日本緩和医療学会
  • 日本臨床腫瘍学会
  • 日本泌尿器腫瘍学会
  • 日本ハイパーサーミア学会
  • 日本内視鏡外科学会
  • 日本泌尿器内視鏡学会
  • アメリカ泌尿器科学会
  • 日本癌治療学会
  • 日本癌学会
  • 日本泌尿器科学会

研究活動情報

研究キーワード

    ナノテクノロジー, 温熱治療, 前立腺癌

論文

  • Efficacy and safety of cytokines versus first-line sunitinib and second-line axitinib for patients with metastatic renal cell carcinoma (ESCAPE study): A study protocol for phase III randomized sequential open-label study., Kadono Y, Konaka H, Izumi K, Anai S, Fujimoto K, Ishibashi K, Kawai N, Kato T, Iba A, Masumori N, Yoshimura K, Mizokami A, Contemporary clinical trials communications, 15,   2019年09月, 査読有り
  • Improvement in early urinary continence recovery after robotic-assisted radical prostatectomy based on postoperative pelvic anatomic features: a retrospective review., Nakane A, Kubota H, Noda Y, Takeda T, Hirose Y, Okada A, Mizuno K, Kawai N, Tozawa K, Hayashi Y, Yasui T, BMC urology, 19, (1) ,   2019年09月, 査読有り
  • NCL1, A Highly Selective Lysine-Specific Demethylase 1 Inhibitor, Suppresses Castration-Resistant Prostate Cancer Growth via Regulation of Apoptosis and Autophagy., Toshiki Etani, Taku Naiki, Aya Naiki-Ito, Takayoshi Suzuki, Keitaro Iida, Satoshi Nozaki, Hiroyuki Kato, Yuko Nagayasu, Shugo Suzuki, Noriyasu Kawai, Takahiro Yasui, Satoru Takahashi, Journal of clinical medicine, 8, (4) ,   2019年03月31日, 査読有り, Recent studies have shown that epigenetic alterations lead to oncogenic activation, thus indicating that these are therapeutic targets. Herein, we analyzed the efficacy and therapeutic potential of our developed histone lysine demethylase 1 (LSD1) inhibitor, NCL1, in castration-resistant prostate cancer (CRPC). The CRPC cell lines 22Rv1, PC3, and PCai1CS were treated with NCL1, and LSD1 expression and cell viability were assessed. The epigenetic effects and mechanisms of NCL1 were also evaluated. CRPC cells showed strong LSD1 expression, and cell viability was decreased by NCL1 in a dose-dependent manner. Chromatin immunoprecipitation analysis indicated that NCL1 induced histone H3 lysine 9 dimethylation accumulation at promoters of P21. As shown by Western blot and flow cytometry analyses, NCL1 also dose-dependently induced caspase-dependent apoptosis. The stimulation of autophagy was observed in NCL1-treated 22Rv1 cells by transmission electron microscopy and LysoTracker analysis. Furthermore, WST-8 assay revealed that the anti-tumor effect of NCL1 was reinforced when autophagy was inhibited by chloroquine in 22Rv1 cells. Combination index analysis revealed that a concurrent use of these drugs had a synergistic effect. In ex vivo analysis, castrated nude mice were injected subcutaneously with PCai1 cells and intraperitoneally with NCL1. Tumor volume was found to be reduced with no adverse effects in NCL1-treated mice compared with controls. Finally, immunohistochemical analysis using consecutive human specimens in pre- and post-androgen deprivation therapy demonstrated that LSD1 expression levels in CRPC, including neuroendocrine differentiation cases, were very high, and identical to levels observed in previously examined prostate biopsy specimens. NCL1 effectively suppressed prostate cancer growth in vitro and ex vivo without adverse events via the regulation of apoptosis and autophagy, suggesting that NCL1 is a potential therapeutic agent for CRPC.
  • Long-term survival of a patient with pulmonary metastatic urothelial carcinoma following metastasectomy., Hasebe K, Naiki T, Oda R, Etani T, Iida K, Sugiyama Y, Nozaki S, Ando R, Kawai N, Nakanishi R, Yasui T, Urology case reports, 21, 52 - 55,   2018年11月, 査読有り
  • A kit ligand, stem cell factor as a possible mediator inducing overactive bladder., Kubota Y, Hamakawa T, Osaga S, Okada A, Hamamoto S, Kawai N, Kohri K, Yasui T, Neurourology and urodynamics, 37, (4) 1258 - 1265,   2018年04月, 査読有り
  • GPX2 promotes development of bladder cancer with squamous cell differentiation through the control of apoptosis., Taku Naiki, Aya Naiki-Ito, Keitaro Iida, Toshiki Etani, Hiroyuki Kato, Shugo Suzuki, Yoriko Yamashita, Noriyasu Kawai, Takahiro Yasui, Satoru Takahashi, Oncotarget, 9, (22) 15847 - 15859,   2018年03月23日, 査読有り, Herein, we elucidated the molecular mechanisms and therapeutic potential of glutathione peroxidase 2 (GPX2) in bladder cancer. GPX2 expression gradually increased during progression from normal to papillary or nodular hyperplasia (PNHP) and urothelial carcinoma (UC) in a rat N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)-induced bladder carcinogenesis model. GPX2 overexpression was more marked in UC with squamous differentiation (SqD) than in pure UC. Clinical intraepithelial lesions of papillary UC and invasive UC with SqD also had strong GPX2 expression in human radical cystectomy specimens. In addition, prognostic analysis using transurethral specimens revealed that low expression level of GPX2 predicted poor prognosis in patients with pure UC. Further, UC cell lines, BC31 and RT4, cultured in vitro also overexpressed GPX2. Knock-down of GPX2 induced significant inhibition of intracellular reactive oxygen species (ROS) production, in addition to significant growth inhibition and increased apoptosis with activation of caspase 3 or 7 in both BC31 and RT4 cells. Interestingly, tumor growth of BC31 cells subcutaneously transplanted in nude mice was significantly caused the induction of apoptosis, as well as inhibition of angiogenesis and SqD by GPX2 down-regulation. Our findings demonstrated that GPX2 plays an important role in bladder carcinogenesis through the regulation of apoptosis against intracellular ROS, and may be considered as a novel biomarker or therapeutic target in bladder cancer.
  • Early abiraterone acetate treatment is beneficial in Japanese castration-resistant prostate cancer after failure of primary combined androgen blockade., Nagai T, Naiki T, Iida K, Etani T, Ando R, Hamamoto S, Sugiyama Y, Akita H, Kubota H, Hashimoto Y, Kawai N, Yasui T, Prostate international, 6, (1) 18 - 23,   2018年03月, 査読有り
  • Gemcitabine and docetaxel as second-line chemotherapy in elderly patients with metastatic urothelial carcinoma: a retrospective analysis., Naiki T, Iida K, Etani T, Nagai T, Tanaka Y, Sugiyama Y, Ando R, Hamamoto S, Banno R, Nagata D, Kawai N, Yasui T, Cancer management and research, 10, 3669 - 3677,   2018年, 査読有り
  • Complete remission of metastatic pheochromocytoma in 123I-metaiodobenzylguanidine scintigraphy after a single session of 131I-metaiodobenzylguanidine therapy: a case report., Sugino T, Ando R, Unno R, Iida K, Naiki T, Hamamoto S, Mizuno K, Okada A, Umemoto Y, Kawai N, Tozawa K, Hayashi Y, Inaki A, Kayano D, Kinuya S, Yasui T, BMC research notes, 10, (1) ,   2017年12月, 査読有り
  • New steps of robot-assisted radical prostatectomy using the extraperitoneal approach: a propensity-score matched comparison between extraperitoneal and transperitoneal approach in Japanese patients, Satoshi Kurokawa, Yukihiro Umemoto, Kentaro Mizuno, Atsushi Okada, Akihiro Nakane, Hidenori Nishio, Shuzo Hamamoto, Ryosuke Ando, Noriyasu Kawai, Keiichi Tozawa, Yutaro Hayashi, Takahiro Yasui, BMC UROLOGY, 17, (1) ,   2017年11月, 査読有り, Background: Robot-assisted radical prostatectomy (RARP) is commonly performed using the transperitoneal (TP) approach with six trocars over an 8-cm distance in the steep Trendelenburg position. In this study, we investigated the feasibility and the benefit of using the extraperitoneal (EP) approach with six trocars over a 4-cm distance in a flat or 5 degrees Trendelenburg position. We also introduced four new steps to the surgical procedure and compared the surgical results and complications between the EP and TP approach using propensity score matching. Methods: Between August 2012 and August 2016, 200 consecutive patients without any physical restrictions underwent RARP with the EP approach in a less than 5 degrees Trendelenburg position, and 428 consecutive patients underwent RARP with the TP approach in a steep Trendelenburg position. Four new steps to RARP using the EP approach were developed: 1) arranging six trocars; 2) creating the EP space using laparoscopic forceps; 3) holding the separated prostate in the EP space outside the robotic view; and 4) preventing a postoperative inguinal hernia. Clinicopathological results and complications were compared between the EP and TP approaches using propensity score matching. Propensity scores were calculated for each patient using multivariate logistic regression based on the preoperative covariates. Results: All 200 patients safely underwent RARP using the EP approach. The mean volume of estimated blood loss and duration of indwelling urethral catheter use were significantly lower with the EP approach than the TP approach (139.9 vs 184.9 mL, p = 0.03 and 5.6 vs 7.7 days, p < 0.01, respectively). No significant differences in the positive surgical margin were observed. None of the patients developed an inguinal hernia postoperatively after we introduced this technique. Conclusions: The EP approach to RARP was safely performed regardless of patient physique or contraindications to a steep Trendelenburg position. Our method, which involved using the EP approach to perform RARP, can decrease the amount of perioperative blood loss, the duration of indwelling urethral catheter use, and the incidence of postoperative inguinal hernia development.
  • A pilot study of gemcitabine and paclitaxel as third-line chemotherapy in metastatic urothelial carcinoma., Naiki T, Iida K, Kawai N, Etani T, Ando R, Nagai T, Tanaka Y, Hamamoto S, Hamakawa T, Akita H, Sugiyama Y, Yasui T, Journal of rural medicine : JRM, 12, (2) 105 - 111,   2017年11月, 査読有り
  • Feasibility of metronomic chemotherapy with tegafur-uracil, cisplatin, and dexamethasone for docetaxel-refractory prostate cancer., Kubota H, Fukuta K, Yamada K, Hirose M, Naruyama H, Yanai Y, Yamada Y, Watase H, Kawai N, Tozawa K, Yasui T, Journal of rural medicine : JRM, 12, (2) 112 - 119,   2017年11月, 査読有り
  • Antimicrobial susceptibility of pathogens in acute uncomplicated cystitis cases in the urology department of a community hospital in Japan: Comparison with treatment outcome and hospital-wide antibiogram, Toshiki Etani, Taku Naiki, Sachiyo Yamaguchi, Saori Mori, Takashi Nagai, Keitaro Iida, Ryosuke Ando, Noriyasu Kawai, Keiichi Tozawa, Tohru Mogami, Takahiro Yasui, JOURNAL OF INFECTION AND CHEMOTHERAPY, 23, (10) 692 - 697,   2017年10月, 査読有り, We hypothesized that cases of uncomplicated cystitis treated in a Urology Department would display higher antimicrobial susceptibility than those reported by the hospital antibiogram. This would suggest narrow spectrum antibiotics could still be an effective treatment for uncomplicated cystitis despite this era of antimicrobial resistance. The objective of this study was thus to evaluate the rates of antimicrobial susceptibility of isolates cultured from uncomplicated cystitis cases that presented to the Urology Department of a community hospital in Japan. We evaluated the efficacy of cefaclor, a narrow spectrum antibiotic, for uncomplicated cystitis. We further compared the rates of antimicrobial susceptibility of isolates from uncomplicated cystitis cases to those reported in a hospital-wide antibiogram. A retrospective chart review was performed of patients diagnosed with uncomplicated cystitis in the Urology Department. The patients were mainly treated orally by cefaclor at 750 mg/day for seven days. Significantly greater susceptibilities to cefazolin (87.0% vs 65.7%), trimethoprim-sulfamethoxazole (89.4% vs 79.1%) and levofloxacin (84.6% vs 66.9%) were observed in a cystitis antibiogram for Escherichia coli compared with a hospital-wide antibiogram. The clinical efficacy of cefaclor for acute cystitis was also demonstrated. The greater susceptibility of Escherichia coli to antimicrobials observed in this study supports the hypothesis that antimicrobial susceptibility rates in uncomplicated cystitis cases that present to the Urology Department would be greater than those reported in the hospital antibiogram. Therefore, uncomplicated acute cystitis can be treated by narrow spectrum antibiotics such as cefaclor even in this "antimicrobial resistance era''. (C) 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
  • Enzalutamide versus abiraterone as a first-line endocrine therapy for castration-resistant prostate cancer (ENABLE study for PCa): a study protocol for a multicenter randomized phase III trial, Kouji Izumi, Atsushi Mizokami, Mikio Namiki, Shogo Inoue, Nobumichi Tanaka, Yuko Yoshio, Kei Ishibashi, Manabu Kamiyama, Noriyasu Kawai, Hideki Enokida, Takashi Shima, Shizuko Takahara, BMC CANCER, 17, (1) ,   2017年10月, 査読有り, Background: Both enzalutamide and abiraterone have demonstrated improved radiographic progression-free and overall survival for castration-resistant prostate cancer (CRPC) compared with placebo controls before docetaxel treatment in phase III studies. These oral agents target androgen and androgen receptor signaling and are thought to be less toxic than chemotherapy. Cross-resistance to these agents was recently reported because of their similar mechanism of action, and it is important to assess which agent is more effective to use initially for CRPC. Methods/design: The present study is a phase III, investigator-initiated, multicenter, head-to-head, randomized controlled trial investigating enzalutamide vs. abiraterone as a first-line treatment for CRPC patients. Patients will be randomly assigned to an enzalutamide or an abiraterone treatment group. The primary endpoint is the time to prostate-specific antigen progression. The target sample size is set at 100 patients per group (total, 200 patients). The study duration is 5 years, and the duration for recruitment is 2 years and 6 months. Discussion: Thus far, there have been no prospective head-to-head studies comparing enzalutamide and abiraterone. This ENABLE study will clarify which agent should be prioritized for CRPC patients and enable clinicians to decide the appropriate treatment before chemotherapy.
  • Robot-assisted laparoscopic pyeloplasty for ureteropelvic junction obstruction: comparison between pediatric and adult patients-Japanese series., Mizuno K, Kojima Y, Kurokawa S, Kamisawa H, Nishio H, Moritoki Y, Nakane A, Maruyama T, Okada A, Kawai N, Tozawa K, Kohri K, Yasui T, Hayashi Y, Journal of robotic surgery, 11, (2) 151 - 157,   2017年06月, 査読有り
  • A Case of Delayed Radiation Myelopathy of the Thoracic Vertebrae Following Low Dose Radiation Therapy for Metastatic Renal Cell Carcinoma., Nozaki S, Naiki T, Hamamoto S, Ando R, Iida K, Kawai N, Mizuno K, Okada A, Umemoto Y, Yasui T, Urology case reports, 11, 66 - 68,   2017年02月, 査読有り
  • Genome-Wide Gene Expression Profiling of Randall's Plaques in Calcium Oxalate Stone Formers, Kazumi Taguchi, Shuzo Hamamoto, Atsushi Okada, Rei Unno, Hideyuki Kamisawa, Taku Naiki, Ryosuke Ando, Kentaro Mizuno, Noriyasu Kawai, Keiichi Tozawa, Kenjiro Kohri, Takahiro Yasui, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 28, (1) 333 - 347,   2017年01月, 査読有り, Randall plaques (RPs) can contribute to the formation of idiopathic calcium oxalate (CaOx) kidney stones; however, genes related to RP formation have not been identified. We previously reported the potential therapeutic role of osteopontin (OPN) and macrophages in CaOx kidney stone formation, discovered using genome-recombined mice and genome-wide analyses. Here, to characterize the genetic pathogenesis of RPs, we used microarrays and immunohistology to compare gene expression among renal papillary RP and non-RP tissues of 23 CaOx stone formers (SFs) (age- and sex-matched) and normal papillary tissue of seven controls. Transmission electron microscopy showed OPN and collagen expression inside and around RPs, respectively. Cluster analysis revealed that the papillary gene expression of CaOx SFs differed significantly from that of controls. Disease and function analysis of gene expression revealed activation of cellular hyperpolarization, reproductive development, and molecular transport in papillary tissue from RPs and non-RP regions of CaOx SFs. Compared with non-RP tissue, RP tissue showed upregulation (>2-fold) of LCN2, IL11, PTGS1, GPX3, and MMD and downregulation (0.5-fold) of SLC12A1 and NALCN (P<0.01). In network and toxicity analyses, these genes associated with activated mitogenactivated protein kinase, the Akt/phosphatidylinositol 3-kinase pathway, and proinflammatory cytokines that cause renal injury and oxidative stress. Additionally, expression of proinflammatory cytokines, numbers of immune cells, and cellular apoptosis increased in RP tissue. This study establishes an association between genes related to renal dysfunction, proinflammation, oxidative stress, and ion transport and RP development in CaOx SFs.
  • Metastatic urothelial carcinoma with glandular differentiation that confirmed the response by autopsy specimen to second-line mFOLFOX6 (Fluorouracil, Oxaliplatin, and Leucovorin) plus bevacizumab chemotherapy, Naiki, T., Etani, T., Naiki-Ito, A., Fujii, K., Ando, R., Iida, K., Nagai, T., Sugiyama, Y., Nakagawa, M., Kawai, N., Yasui, T., Case Reports in Oncology, 10, (3) 1057 - 1064,   2017年, 査読有り
  • Genomic Landscape of Experimental Bladder Cancer in Rodents and Its Application to Human Bladder Cancer: Gene Amplification and Potential Overexpression of Cyp2a5/CYP2A6 Are Associated with the Invasive Phenotype, Kazuhiro Kanemoto, Katsuhiro Fukuta, Noriyasu Kawai, Keiichi Tozawa, Masako Ochiai, Koji Okamoto, Sumiko Ohnami, Hiromi Sakamoto, Teruhiko Yoshida, Yae Kanai, Masaru Katoh, Takahiro Yasui, Kenjiro Kohri, Tadao Kakizoe, Hitoshi Nakagama, PLOS ONE, 11, (11) ,   2016年11月, 査読有り, Non-muscle invasive (superficial) bladder cancer is a low-grade malignancy with good prognosis, while muscle invasive (invasive) bladder cancer is a high-grade malignancy with poor prognosis. N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induces superficial bladder cancers with papillary morphology in rats and invasive bladder cancers with infiltrating phenotype in mice. In this study, we analyzed genomic landscapes of rodent BBN-induced bladder cancers using array-based comparative genomic hybridization (array CGH). While no significant copy number alterations were detected in superficial bladder tumors in rats, copy number gains in chromosomal regions 2D-E1, 7qA3, 9F2, and 11C-D were detected in invasive bladder tumors in mice. Amplification of representative genes located on 2D-E1 and 7qA3 chromosomal regions was confirmed by quantitative PCR. Cyp2a22 and Cyp2a5 genes but not Cyp2g1, Cyp2a12, and Rab4b genes on mouse chromosome 7qA3 were amplified in invasive bladder cancers. Although the human ortholog gene of Cyp2a22 has not been confirmed, the mouse Cyp2a5 gene is the ortholog of the human CYP2A6 gene located in chromosomal region 19q13.2, and CYP2A6 was identified by database search as one of the closest human homolog to mouse Cyp2a22. Considering a possibility that this region may be related to mouse 7qA3, we analyzed CYP2A6 copy number and expression in human bladder cancer using cell lines and resected tumor specimens. Although only one of eight cell lines showed more than one copy increase of the CYP2A6 gene, CYP2A6 amplification was detected in six out of 18 primary bladder tumors where it was associated with the invasive phenotype. Immunohistochemical analyses of 118 primary bladder tumors revealed that CYP2A6 protein expression was also higher in invasive tumors, especially in those of the scattered type. Together, these findings indicate that the amplification and overexpression of the CYP2A6 gene are characteristic of human bladder cancers with increased malignancy and that CYP2A6 can be a candidate prognostic biomarker in this type of cancer.
  • Treatment Strategy for Pediatric Paratesticular Rhabdomyosarcoma Based on Chimeric Gene Assessment, Rei Unno, Kentaro Mizuno, Yasuhiko Ito, Toshiki Etani, Atsushi Okada, Noriyasu Kawai, Takahiro Yasui, Shinji Saitoh, Yutaro Hayashi, UROLOGY, 95, 187 - 189,   2016年09月, 査読有り, Rhabdomyosarcoma (RMS), a malignant tumor of the soft tissue, occurs in two major subtypes: embryonal and alveolar. A majority of pediatric RMS cases involve the embryonal type and occur in the soft tissues of the head and neck or the urogenital organs, which contain paratesticular tissues. We report herein two cases of pediatric paratesticular RMS. One case was embryonal, whereas the other case was alveolar; the latter exhibited PAX7-FOXO1 gene chimerism and rapid progression. Notably, this is the first report of pediatric paratesticular pure-type alveolar RMS in Japan. (C) 2016 Elsevier Inc.
  • Nonpalpable testicular pure seminoma with elevated serum alpha-fetoprotein presenting with retroperitoneal metastasis: a case report., Iwatsuki S, Naiki T, Kawai N, Etani T, Iida K, Ando R, Nagai T, Okada A, Tozawa K, Sugiyama Y, Yasui T, Journal of medical case reports, 10, (1) ,   2016年05月, 査読有り
  • [A Case of Spindle Cell Lipoma in the Inguinal Region]., Takeda T, Ando R, Unno R, Iida K, Iwatsuki S, Umemoto Y, Kawai N, Tozawa K, Yasui T, Hinyokika kiyo. Acta urologica Japonica, 62, (4) 205 - 208,   2016年04月, 査読有り
  • Bacillus Calmette-Guerin therapy after the second transurethral resection significantly decreases recurrence in patients with new onset high-grade T1 bladder cancer, Keitaro Iida, Taku Naiki, Noriyasu Kawai, Toshiki Etani, Ryosuke Ando, Yosuke Ikegami, Takehiko Okamura, Hiroki Kubota, Atsushi Okada, Kenjiro Kohri, Takahiro Yasui, BMC UROLOGY, 16,   2016年02月, 査読有り, Background: The purpose of this study was to assess the efficacy of Bacillus Calmette-Guerin (BCG) therapy after a second transurethral resection (TUR) in new onset high-grade T1 bladder cancer. Methods: From January 2008 to September 2013, 207 patients with new onset high-grade T1 bladder cancer after an initial TUR were treated at our university and at affiliated hospitals. Residual cancer rate, intravesical recurrence-free survival (RFS), and risk factors for intravesical recurrence were analyzed. Results: Among a total of 207 patients, 42 patients were treated with BCG therapy following a second TUR (group 1), 23 were treated with second TUR alone (group 2), 72 were treated with BCG alone (group 3), and 70 were treated without a second TUR or BCG. The median patients' age was 72.0 years, and the median follow-up period was 33.5 months. The second TUR revealed that 34 patients (52 %) had residual cancer. Between groups 1 and 2 and groups 1 and 3, the differences in RFS were statistically significant (p = 0.002 and 0.045, respectively). In addition, BCG therapy was the most significant factor to predict RFS after the second TUR. Among the 31 patients whose pathology of the second TUR was pT0, only 1 of 12 patients (8 %) in group 1 and 11 of 19 patients (58 %) in group 2 had a recurrence. Conclusions: BCG instillation following a second TUR decreases intravesical recurrence, even if the pathology of the second TUR is pT0.
  • Pure Lymphoepithelioma-Like Carcinoma Originating from the Urinary Bladder., Nagai T, Naiki T, Kawai N, Iida K, Etani T, Ando R, Hamamoto S, Sugiyama Y, Okada A, Mizuno K, Umemoto Y, Yasui T, Case reports in oncology, 9, (1) 188 - 194,   2016年01月, 査読有り
  • Adrenal Neuroblastoma in an Adult: Effect of Radiotherapy on Local Progression after Surgical Removal., Kurokawa S, Mizuno K, Nakane A, Moritoki Y, Nishio H, Kamisawa H, Kubota Y, Okada A, Kawai N, Hayashi Y, Yasui T, Case reports in urology, 2016,   2016年, 査読有り
  • Inflammatory Myofibroblastic Tumor of the Urinary Bladder: A Case Report, Etani, T., Naiki, T., Nagai, T., Iida, K., Ando, R., Naiki-Ito, A., Kawai, N., Tozawa, K., Mizuno, K., Okada, A., Mogami, T., Yasui, T., Case Reports in Oncology, 9, (2) 464 - 469,   2016年, 査読有り
  • Genome-Wide Gene Expression Profiling of Randall's Plaques in Calcium Oxalate Stone Formers., Taguchi K, Hamamoto S, Okada A, Unno R, Kamisawa H, Naiki T, Ando R, Mizuno K, Kawai N, Tozawa K, Kohri K, Yasui T, J Am Soc Nephrol,   2016年
  • Neuroendocrine Carcinoma of the Kidney and Bladder with Loss of Heterozygosity and Changes in Chromosome 3 Copy Number., Okada A, Iida K, Hamakawa T, Umemoto Y, Yasui T, Kawai N, Tozawa K, Sasaki S, Hayashi Y, Kohri K, The American journal of case reports, 16, 611 - 616,   2015年09月, 査読有り
  • MRI Findings of Inverted Urothelial Papilloma of the Bladder, Mitsuru Takeuchi, Kohei Sasaguri, Taku Naiki, Akihito Mitsumori, Hirotoshi Ito, Junko Takahama, Kaori Yamada, Nagaaki Marugami, Takahiro Tsuboyama, Yoshihiro Okumura, Yoshimitstu Ohgiya, Noriyasu Kawai, Kenjiro Kohri, Yuta Shibamoto, AMERICAN JOURNAL OF ROENTGENOLOGY, 205, (2) 311 - 316,   2015年08月, 査読有り, OBJECTIVE. The objective of this study was to characterize MRI findings of inverted urothelial papilloma of the bladder. MATERIALS AND METHODS. Data pertaining to 16 patients with 18 pathologically proven inverted urothelial papillomas of the bladder who had undergone MRI were retrospectively collected from seven institutions. The shape and surface characteristics of the tumors were evaluated using T2-weighted MR images. In addition, the signal intensity of inverted urothelial papillomas was visually assessed on Tl-weighted, T2-weighted, and DW images and on early and delayed phase contrast-enhanced images. RESULTS. The shape of the 18 inverted urothelial papillomas of the bladder was classified as polypoid with a stalk for 16 tumors (89%) and polypoid without a stalk for two tumors (11%). All stalks were surrounded by urine in the bladder. A total of 15 of the tumor surfaces (83%) were nonpapillary and three (17%) were papillary. All 12 of the inverted urothelial papillomas for which evaluable T1-weighted images were available were isointense with the bladder wall. The lesions had a slightly higher signal intensity than the bladder wall in 15 of the patients (83%) and showed isointensity with the bladder wall in three patients (17%). A total of three patients (17%) had tiny hyperintense foci noted on T2-weighted images. All 16 of the inverted urothelial papillomas examined by DWI had very high signal intensity. All 13 of the lesions for which early phase images were obtained using dynamic contrast-enhanced MRI showed strong enhancement. When compared with early phase images, delayed phase images of the same 13 lesions showed that enhancement was stronger in two lesions (15%), similar in six lesions (46%), and weaker in five lesions (38%). CONCLUSION. On MRI, the typical appearance of inverted urothelial papillomas of the bladder is a polypoid shape with a nonpapillary surface and a thin short stalk surrounded by urine. Cystic foci are also occasionally seen within the tumor.
  • [A case of abscess of corpus cavernosum as an early symptom of penile pyodermal gangrenosum: we propose the possibility of a new pathogenic finding]., Iida K, Mizuno K, Kawai N, Ito E, Shintani Y, Morita A, Khori K, Hinyokika kiyo. Acta urologica Japonica, 61, (3) 115 - 119,   2015年03月, 査読有り
  • NCL1, a highly selective lysine-specific demethylase 1 inhibitor, suppresses prostate cancer without adverse effect., Etani T, Suzuki T, Naiki T, Naiki-Ito A, Ando R, Iida K, Kawai N, Tozawa K, Miyata N, Kohri K, Takahashi S, Oncotarget, 6, (5) 2865 - 2878,   2015年02月, 査読有り
  • MRI for differentiation of renal cell carcinoma with sarcomatoid component from other renal tumor types, Mitsuru Takeuchi, Tatsuya Kawai, Tomohiro Suzuki, Taku Naiki, Noriyasu Kawai, Yukio Fujiyoshi, Hiroshi Inagaki, Kenjiro Kohri, Masaki Hara, Yuta Shibamoto, ABDOMINAL IMAGING, 40, (1) 112 - 119,   2015年01月, 査読有り, To investigate the usefulness of MRI for detection of sarcomatoid renal cell carcinoma (SRCC) components within RCC and differentiation from other renal tumors. Two observers independently interpreted T2-weighted images of 10 patients with pathologically confirmed RCCs with SRCC and 131 with non-SRCC renal tumors, with special reference to conspicuously low signal intensity (SI) areas (T2LIA) compared to the renal cortex. SRCC probability was classified as (1) definitely non-SRCC, no T2LIA; (2) probably non-SRCC, < 1 cm T2LIA; (3) low probability of SRCC, homogeneous tumor with 1-3 cm T2LIA; (4) probably SRCC, heterogeneous tumor with 1-3 cm T2LIA; and (5) definitely SRCC, > 3 cm T2LIA, multiple > 1 cm T2LIAs, or showing disruption of the pseudocapsule. The observers used chemical shift imaging to exclude the area representing hemorrhage or hemosiderin deposition from T2LIA. Scores of 4/5 were regarded as positive for evaluating the accuracy and area under the receiver operating characteristic curve. The SI ratio of the lowest SI in the tumor to that of the renal cortex in the 1 and a parts per thousand yen2 score groups was compared using Mann-Whitney's U test. Sensitivity, specificity, accuracy, and positive and negative predictive values were 90%, 95%, 94%, 56%, and 99%, respectively, and area under the receiver operating characteristic curve was 0.93. The mean SI ratio of the lowest SI in the tumor to that of the renal cortex was significantly lower in the a parts per thousand yen2 score group (0.58) than in the 1 score group (1.36). MRI predicted RCC with SRCC with a moderate positive predictive value and a high negative predictive value.
  • A Case of Renal Primitive Neuroectodermal Tumor Confirmed by Fluorescence in situ Hybridization., Etani T, Naiki T, Ando R, Iida K, Naiki-Ito A, Takahashi S, Kobayashi D, Kawai N, Tozawa K, Yasui T, Kohri K, Case reports in oncology, 8, (1) 205 - 211,   2015年, 査読有り
  • A Case of Metastatic Urothelial Carcinoma Treated with Pemetrexed as Third-Line Chemotherapy with Discussion and Literature Review., Iida K, Kawai N, Naiki T, Etani T, Ando R, Nagai T, Sugiyama Y, Naiki-Ito A, Nishio H, Okada A, Kohri K, Yasui T, Case reports in oncology, 8, (3) 530 - 535,   2015年, 査読有り
  • Laparoscopic Versus Open Radical Cystectomy for Patients Older than 75 Years: a Single-Center Comparative Analysis., Yasui T, Tozawa K, Ando R, Hamakawa T, Iwatsuki S, Taguchi K, Kobayashi D, Naiki T, Mizuno K, Okada A, Umemoto Y, Kawai N, Sasaki S, Hayashi Y, Kohri K, Asian Pacific journal of cancer prevention : APJCP, 16, (15) 6353 - 6358,   2015年, 査読有り
  • Efficacy of endoscopic combined intrarenal surgery in the prone split-leg position for staghorn calculi, Hamamoto S, Yasui T, Okada A, Koiwa S, Taguchi K, Itoh Y, Kawai N, Hashimoto Y, Tozawa K, Kohri K, J Endourol, 29, (1) 19 - 24,   2015年01月, 査読有り
  • Laparoscopic Versus Open Radical Cystectomy for Patients Older than 75 Years: a Single-Center Comparative Analysis, Yasui T, Tozawa K, Ando R, Hamakawa T, Iwatsuki S, Taguchi K, Kobayashi D, Naiki T, Mizuno K, Okada A, Umemoto Y, Kawai N, Sasaki S, Hayashi Y, Kohri K, Asian Pac J Cancer Prev, 16, (15) 6353 - 6358,   2015年, 査読有り
  • Metastasectomy as optimal treatment for late relapsing solitary brain metastasis from testicular germ cell tumor: a case report., Iida K, Naiki T, Kawai N, Ando R, Etani T, Tozawa K, Kohri K, BMC research notes, 7,   2014年12月, 査読有り
  • Pitfalls of robot-assisted radical prostatectomy: A comparison of positive surgical margins between robotic and laparoscopic surgery, Keiichi Tozawa, Takahiro Yasui, Yukihiro Umemoto, Kentaro Mizuno, Atsushi Okada, Noriyasu Kawai, Satoru Takahashi, Kenjiro Kohri, INTERNATIONAL JOURNAL OF UROLOGY, 21, (10) 976 - 979,   2014年10月, 査読有り, ObjectivesTo compare the surgical outcomes of laparoscopic radical prostatectomy and robot-assisted radical prostatectomy, including the frequency and location of positive surgical margins. MethodsThe study cohort comprised 708 consecutive male patients with clinically localized prostate cancer who underwent laparoscopic radical prostatectomy (n=551) or robot-assisted radical prostatectomy (n=157) between January 1999 and September 2012. Operative time, estimated blood loss, complications, and positive surgical margins frequency were compared between laparoscopic radical prostatectomy and robot-assisted radical prostatectomy. ResultsThere were no significant differences in age or body mass index between the laparoscopic radical prostatectomy and robot-assisted radical prostatectomy patients. Prostate-specific antigen levels, Gleason sum and clinical stage of the robot-assisted radical prostatectomy patients were significantly higher than those of the laparoscopic radical prostatectomy patients. Robot-assisted radical prostatectomy patients suffered significantly less bleeding (P<0.05). The overall frequency of positive surgical margins was 30.6% (n=167; 225 sites) in the laparoscopic radical prostatectomy group and 27.5% (n=42; 58 sites) in the robot-assisted radical prostatectomy group. In the laparoscopic radical prostatectomy group, positive surgical margins were detected in the apex (52.0%), anterior (5.3%), posterior (5.3%) and lateral regions (22.7%) of the prostate, as well as in the bladder neck (14.7%). In the robot-assisted radical prostatectomy patients, they were observed in the apex, anterior, posterior, and lateral regions of the prostate in 43.0%, 6.9%, 25.9% and 15.5% of patients, respectively, as well as in the bladder neck in 8.6% of patients. ConclusionsPositive surgical margin distributions after robot-assisted radical prostatectomy and laparoscopic radical prostatectomy are significantly different. The only disadvantage of robot-assisted radical prostatectomy is the lack of tactile feedback. Thus, the robotic surgeon needs to take this into account to minimize the risk of positive surgical margins.
  • GPX2 overexpression is involved in cell proliferation and prognosis of castration-resistant prostate cancer., Naiki T, Naiki-Ito A, Asamoto M, Kawai N, Tozawa K, Etani T, Sato S, Suzuki S, Shirai T, Kohri K, Takahashi S, Carcinogenesis, 35, (9) 1962 - 1967,   2014年09月, 査読有り
  • Developments in the Technique of Endoscopic Combined Intrarenal Surgery in the Prone Split-leg Position, Shuzo Hamamoto, Takahiro Yasui, Atsushi Okada, Mitsuru Takeuchi, Kazumi Taguchi, Yuta Shibamoto, Yutaka Iwase, Noriyasu Kawai, Keiichi Tozawa, Kenjiro Kohri, UROLOGY, 84, (3) 565 - 570,   2014年09月, 査読有り, OBJECTIVE To develop a new technique for performing endoscopic combined intrarenal surgery in the prone split-leg position and to evaluate its efficacy using computed tomography. MATERIALS AND METHODS Between December 2010 and January 2013, 60 patients with large calculi (39.2 +/- 2.6 mm) underwent this surgery. A laser fiber was used with a flexible ureteroscope introduced through a ureteral access sheath, and lithoclast lithotripsy was performed through a mini-percutaneous tract. Three-dimensional computed tomography was performed to determine anatomic variations, including the ureteral location and ureteropelvic junction angle in all patients in both the supine and prone positions. RESULTS All procedures were performed successfully with a single tract and the patient in the prone split-leg position. The mean surgical time was 120.5 +/- 6.7 min. The initial stone-free rate was 82%, and the final stone-free rate was 87% after further treatment. One patient required blood transfusion, but none had severe complications. Computed tomography showed that the ureter between the orifice and ureteropelvic junction was straighter and the ureteropelvic junction angle was significantly smaller for surgeries conducted in the prone position than the supine position. CONCLUSION Flexible ureteroscopy in the prone split-leg position is a viable technique. It allows easy insertion of the ureteral sheath and access of the ureteroscope to the renal pelvis. The findings suggest that this hybrid surgery is an efficient, effective, and versatile procedure for the management of renal calculi. (C) 2014 Elsevier Inc.
  • Colony-Stimulating Factor-1 Signaling Suppresses Renal Crystal Formation, Kazumi Taguchi, Atsushi Okada, Hiroshi Kitamura, Takahiro Yasui, Taku Naiki, Shuzo Hamamoto, Ryosuke Ando, Kentaro Mizuno, Noriyasu Kawai, Keiichi Tozawa, Kenichi Asano, Masato Tanaka, Ichiro Miyoshi, Kenjiro Kohri, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 25, (8) 1680 - 1697,   2014年08月, 査読有り, We recently reported evidence suggesting that migrating macrophages (M phi s) eliminate renal crystals in hyperoxaluric mice. M phi s can be inflammatory (M1) or anti-inflammatory (M2), and colony-stimulating factor-1 (CSF-1) mediates polarization to the M2M phi phenotype. M2M phi s promote renal tissue repair and regeneration, but it is not clear whether these cells are involved in suppressing renal crystal formation. We investigated the role of M2M phi s in renal crystal formation during hyperoxaluria using CSF-1-deficient mice, which lack M2M phi s. Compared with wild-type mice, CSF-1-deficient mice had significantly higher amounts of renal calcium oxalate crystal deposition. Treatment with recombinant human CSF-1 increased the expression of M2-related genes and markedly decreased the number of renal crystals in both CSF-1-deficient and wild-type mice. Flow cytometry of sorted renal M phi s showed that CSF-1 deficiency resulted in a smaller population of CD11b(+)F4/80(+)CD163(+)CD206(hi) cells, which represent M2-like M phi s. Additionally, transfusion of M2M phi s into CSF-1 deficient mice suppressed renal crystal deposition. In vitro phagocytosis assays with calcium oxalate monohydrate crystals showed a higher rate of crystal phagocytosis by M2-polarized M phi s than M1-polarized M phi s or renal tubular cells. Gene array profiling showed that CSF-1 deficiency resulted in disordered M2- and stone-related gene expressions. Collectively, our results provide compelling evidence for a suppressive role of CSF-1 signaling in renal crystal formation.
  • Novel effect of the inhibitor of mitochondrial cyclophilin D activation, N-methyl-4-isoleucine cyclosporin, on renal calcium crystallization., Niimi K, Yasui T, Okada A, Hirose Y, Kubota Y, Umemoto Y, Kawai N, Tozawa K, Kohri K, International journal of urology : official journal of the Japanese Urological Association, 21, (7) 707 - 713,   2014年07月, 査読有り
  • Laparoscopic adrenalectomy for solitary adrenal metastasis from lung cancer, Kawai N, Tozawa K, Yasui T, Moritoki Y, Sasaki H, Yano M, Fujii Y, Kohri K, JSLS, 18, (3) ,   2014年07月, 査読有り
  • Gemcitabine and docetaxel, an effective second-line chemotherapy for lung metastasis of urothelial carcinoma, Taku Naiki, Noriyasu Kawai, Yoshihiro Hashimoto, Takehiko Okamura, Ryosuke Ando, Takahiro Yasui, Atsushi Okada, Toshiki Etani, Keiichi Tozawa, Kenjiro Kohri, INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY, 19, (3) 516 - 522,   2014年06月, 査読有り, The objective of this study was to evaluate the efficacy of a gemcitabine and docetaxel (GD) combination as a second-line treatment for patients with metastatic urothelial carcinoma (UC) after failure of first-line treatment with platinum-based chemotherapy. From June 2006 to January 2012, 38 patients with metastatic UC previously treated with platinum-based chemotherapy received GD therapy. This consisted of gemcitabine 800 mg/m(2) and docetaxel 40 mg/m(2) on days 1 and 8 of each 21-day cycle as second-line chemotherapy. All the patients were evaluated for toxicity and assessed every cycle by imaging. We analyzed the efficacy of GD as second-line chemotherapy in the follow-up study. The median number of GD treatment cycles was 4 (range 2-9); the objective response rate was 47.4 %; and the median progression-free survival and median overall survival were 4.1 and 10.8 months, respectively. Univariate and multivariate analyses on the GD treated group showed that the existence of lung metastases was the only prognostic factor for tumor response. Grade 3 treatment-related toxicity included neutropenia (31.6 %) and thrombocytopenia (15.8 %), and only one patient with grade 4 toxicity had thrombocytopenia (2.6 %). The GD regimen as second-line chemotherapy was especially effective for lung metastatic UC and yielded favorable results in patients whose first-line platinum-based chemotherapy had failed. Given the safety and benefit profile seen in this study, a large prospective study is warranted to consider the potential utility of GD chemotherapy as a second-line for UC.
  • Toxicity and efficacy of three dose-fractionation regimens of intensity-modulated radiation therapy for localized prostate cancer, Yoshihiko Manabe, Yuta Shibamoto, Chikao Sugie, Fumiya Baba, Shiho Ayakawa, Aiko Nagai, Shinya Takemoto, Akihiro Hayashi, Noriyasu Kawai, Mitsuru Takeuchi, Satoshi Ishikura, Kenjiro Kohri, Takeshi Yanagi, JOURNAL OF RADIATION RESEARCH, 55, (3) 494 - 501,   2014年05月, 査読有り, Outcomes of three protocols of intensity-modulated radiation therapy (IMRT) for localized prostate cancer were evaluated. A total of 259 patients treated with 5-field IMRT between 2005 and 2011 were analyzed. First, 74 patients were treated with a daily fraction of 2.0 Gy to a total of 74 Gy (low risk) or 78 Gy (intermediate or high risk). Then, 101 patients were treated with a 2.1-Gy daily fraction to 73.5 or 77.7 Gy. More recently, 84 patients were treated with a 2.2-Gy fraction to 72.6 or 74.8 Gy. The median patient age was 70 years (range, 54-82) and the follow-up period for living patients was 47 months (range, 18-97). Androgen deprivation therapy was given according to patient risk. The overall and biochemical failure-free survival rates were, respectively, 96 and 82% at 6 years in the 2.0-Gy group, 99 and 96% at 4 years in the 2.1-Gy group, and 99 and 96% at 2 years in the 2.2-Gy group. The biochemical failure-free rate for high-risk patients in all groups was 89% at 4 years. Incidences of Grade a parts per thousand yen2 acute genitourinary toxicities were 9.5% in the 2.0-Gy group, 18% in the 2.1-Gy group, and 15% in the 2.2-Gy group (P = 0.29). Cumulative incidences of Grade a parts per thousand yen2 late gastrointestinal toxicity were 13% in the 2.0-Gy group at 6 years, 12% in the 2.1-Gy group at 4 years, and 3.7% in the 2.2-Gy group at 2 years (P = 0.23). So far, this stepwise shortening of treatment periods seems to be successful.
  • Increased crystal-cell interaction in vitro under co-culture of renal tubular cells and adipocytes by in vitro co-culture paracrine systems simulating metabolic syndrome, Jun Ichikawa, Atsushi Okada, Kazumi Taguchi, Yasuhiro Fujii, Li Zuo, Kazuhiro Niimi, Shuzo Hamamoto, Yasue Kubota, Yukihiro Umemoto, Yasunori Itoh, Takahiro Yasui, Noriyasu Kawai, Keiichi Tozawa, Kenjiro Kohri, UROLITHIASIS, 42, (1) 17 - 28,   2014年02月, 査読有り, We established an experimental co-culture system for renal tubular cells and adipocytes to investigate kidney stone formation mechanisms under metabolic syndrome (MetS) conditions and examined the interaction between these cells morphologically and genetically. M-1s and 3T3-L1s were cultured individually (control, CON), with 24-h culture media from each cell type added to the other cell type (replacement, RP) in 2-layer co-culture dishes for 24 h (transwell, TW). M-1s were then exposed to calcium oxalate monohydrate (COM) crystals, and attached C-14-labeled COM crystals were quantified. Expression of kidney stone- and adipocyte-related genes was analyzed. The radioactivity of adherent COM crystals significantly increased in TW and was relatively higher in RP compared to CON. M-1s demonstrated significant upregulation of adiponectin (Adipoq) in RP and secreted phosphoprotein 1 (Spp1) in TW compared to CON before COM crystal exposure, and significant downregulation of Spp1 in TW and upregulation of tumor necrosis factor (Tnf), interleukin 6 (Il-6), and chemokine (C-C motif) ligand 2 (Ccl2) compared to CON after COM crystal exposure. 3T3-L1s showed significant upregulation of Spp1, Adipoq, Tnf-alpha, and Ccl2 compared to CON. Enzyme-linked immunosorbent assays of co-culture medium revealed significantly increased TNF-alpha in TW. Our results highlight the potential for paracrine interactions between renal tubular cells and adipocytes and suggest that MetS conditions may lead to kidney stone formation.
  • Long-term follow-up of nephrotoxicity in rats administered both melamine and cyanuric acid., Yasui T, Kobayashi T, Okada A, Hamamoto S, Hirose M, Mizuno K, Kubota Y, Umemoto Y, Kawai N, Tozawa K, Gao B, Kohri K, BMC research notes, 7,   2014年02月, 査読有り
  • Laparoendoscopic Single-Site Partial Nephrectomy Without Hilar Clamping Using a Microwave Tissue Coagulator, Noriyasu Kawai, Takahiro Yasui, Yukihiro Umemoto, Yasue Kubota, Kentaro Mizuno, Atsushi Okada, Ryosuke Ando, Keiichi Tozawa, Yutaro Hayashi, Kenjiro Kohri, JOURNAL OF ENDOUROLOGY, 28, (2) 184 - 190,   2014年02月, 査読有り, Purpose: To report our initial experience and evaluate the possibility of nonhilar clamp laparoendoscopic single-site (LESS) partial nephrectomy by using a microwave tissue coagulator. Patients and Methods: From December 2010 to May 2012, all patients with an exophytic, solitary, enhancing small (4.0cm) renal mass were chosen to receive the study treatment. A multichannel port provided both a retroperitoneal and transperitoneal approach. A rigid, articulating apparatus was used to perform dissection, exposure of tumor, tissue coagulation, and resection without hilar clamping. Pathologic and hematologic data, subjective evaluation of pain, and scar appearance were analyzed. Results: Nonhilar clamp LESS partial nephrectomy by using a microwave tissue coagulator was performed in seven patients (mean operative time, 208min; mean blood loss, 39mL; mean renal mass size, 1.7cm); one procedure with uncontrolled bleeding needed to be converted to conventional laparoscopic partial nephrectomy. No transfusion was necessary. Pathologic investigation demonstrated six renal-cell carcinomas and one oncocytoma. The hemoglobin level decreased by a mean of 1.1g/dL. Patients did not complain about pain, and they had great satisfaction with the results. The mean duration of hospital stay was 13.8 days. Conclusions: LESS partial nephrectomy without hilar clamping by using a microwave tissue coagulator is possible for renal masses, confers postoperative outcomes comparable to the standard counterpart, and assures patient satisfaction. By use of a microwave tissue coagulator, additional trocars were not necessary, and LESS partial nephrectomy could be accomplished through a single port, which reduced invasion and increased the cosmetic satisfaction of the patients.
  • Endoscopic Combined Intrarenal Surgery for Large Calculi: Simultaneous Use of Flexible Ureteroscopy and Mini-Percutaneous Nephrolithotomy Overcomes the Disadvantageous of Percutaneous Nephrolithotomy Monotherapy, Shuzo Hamamoto, Takahiro Yasui, Atsushi Okada, Kazumi Taguchi, Noriyasu Kawai, Ryosuke Ando, Kentaro Mizuno, Yasue Kubota, Hiroyuki Kamiya, Keiichi Tozawa, Kenjiro Kohri, JOURNAL OF ENDOUROLOGY, 28, (1) 28 - 33,   2014年01月, 査読有り, Background and Purpose: Percutaneous nephrolithotomy (PCNL) is considered the standard procedure for the removal of large renal calculi. The development of the minimally invasive PCNL (mini-PCNL) has reduced the complications of the surgery; it also appears to be associated with less morbidity than the conventional PCNL (con-PCNL). This study aimed at evaluating the efficacy of endoscopic intrarenal surgery, using the prone-split leg position, using flexible ureteroscopy and mini-PCNL (mini- endoscopic combined intrarenal surgery [ECIRS]) by retrospectively comparing this technique with mini-PCNL and con-PCNL. Patients and Methods: In total, 161 consecutive patients who were observed for the follow-up of large renal calculi between February 2004 and January 2013 were selected for mini-ECIRS (60), mini-PCNL (19), or con-PCNL (82). Mini-ECIRS was performed with patients in the prone split-leg position via 18F minipercutaneous tract and 14F ureteral access sheath. The mini-PCNL was performed via 18F percutaneous tract and con-PCNL performed via the 30F tract. Mean size of the renal calculi removed via mini-ECIRS, mini-PCNL, and con-PCNL were 39.2, 38.4, and 34.6mm, respectively. Results: Average surgical time for mini-ECIRS was shorter than that for mini-PCNL and con-PCNL (120.5 vs 181.9 vs 134.1min, respectively; P<0.001). The stone-free rate for mini-ECIRS was significantly higher than that of the other procedures (initial rates 81.7% vs 38.9% vs 45.1%, respectively; P<0.001; rates after further treatment 86.7% vs 61.1% vs 61.0%, respectively; P=0.002). Only one patient in the mini-ECIRS group needed blood transfusions. The decrease in hemoglobin during mini-ECIRS and mini-PCNL was significantly lower than that during con-PCNL (P=0.011). Conclusion: Mini-ECIRS is better than monotherapy with mini-PCNL or con-PCNL. The study results show that mini-ECIRS is a safe, efficient, and versatile procedure that can be effective for the management of renal calculi.
  • Outcomes of Robot-Assisted Laparoscopic Prostatectomy with a Posterior Approach to the Seminal Vesicle in 300 Patients., Yasui T, Tozawa K, Okada A, Kurokawa S, Kubota H, Mizuno K, Umemoto Y, Kawai N, Sasaki S, Hayashi Y, Kohri K, International scholarly research notices, 2014,   2014年, 査読有り
  • Impact of prostate weight on perioperative outcomes of robot-assisted laparoscopic prostatectomy with a posterior approach to the seminal vesicle, Takahiro Yasui, Keiichi Tozawa, Satoshi Kurokawa, Atsushi Okada, Kentaro Mizuno, Yukihiro Umemoto, Noriyasu Kawai, Shoichi Sasaki, Yutaro Hayashi, Yoshiyuki Kojima, Kenjiro Kohri, BMC UROLOGY, 14,   2014年01月, 査読有り, Background: To determine the effect of prostate weight on the preoperative and postoperative outcomes of robotic-assisted laparoscopic radical prostatectomy with a posterior approach to the seminal vesicle. Methods: This retrospective study examined prospectively collected data on 219 robotic-assisted laparoscopic radical prostatectomies performed from May 2011 to February 2013. Patients were divided into four groups based on pathologic prostate weight: < 30 g, 30-49 g, 50-79 g, and >= 80 g. Continence and sexual function were assessed using validated questionnaires. Results: Of the 219 patients, 19, 143, 51, and 6 had prostates weighing < 30 g, 30-49 g, 50-79 g, and >= 80 g, respectively. Significant differences were found between the preoperative Gleason scores, total operative times, and robotic times of the groups. Both estimated blood loss and anastomosis time tended to be greater in the higher prostate weight groups, but the differences were not significant. No significant differences were observed in transfusion rate, length of catheterization, complication incidence, or positive surgical margins. The return of urinary function, as determined by questionnaire scores, was not affected by prostate weight. Conclusions: Robotic-assisted laparoscopic radical prostatectomy can be performed safely and with similar perioperative outcomes, regardless of prostate weight. Indeed, oncological outcome, urinary continence, and complications were similar across the prostate weight groups, suggesting that robotic-assisted laparoscopic radical prostatectomy with a posterior approach to the seminal vesicle may be performed effectively on men with large prostates, despite greater surgical times.
  • Evaluation of side effects of radiofrequency capacitive hyperthermia with magnetite on the blood vessel walls of tumor metastaticlesion surrounding the abdominal large vessels: an agar phantom study, Kawai N, Kobayashi D, Yasui T, Umemoto Y, Mizuno K, Okada A, Tozawa K, Kobayashi T, Kohri K, Vasc Cell, 6,   2014年, 査読有り
  • MR Imaging of Urinary Bladder Cancer for T-Staging: A Review and a Pictorial Essay of Diffusion-Weighted Imaging, Mitsuru Takeuchi, Shigeru Sasaki, Taku Naiki, Noriyasu Kawai, Kenjiro Kohri, Masaki Hara, Yuta Shibamoto, JOURNAL OF MAGNETIC RESONANCE IMAGING, 38, (6) 1299 - 1309,   2013年12月, 査読有り, Treatment decisions for bladder cancer patients are mainly based on the depth of bladder wall invasion by the tumor. In this article, we review the conventional MRI and exhibit a recently emerged diffusion-weighted imaging (DWI) of urinary bladder cancer for T-staging. We discuss limitations of conventional MRI, scanning protocols of DWI, normal pelvic findings on DWI, determination of T-stage using DWI, and pitfalls of DWI. DWI provides high contrast between bladder cancer and background tissue because the cancer shows markedly high SI. DWI has high sensitivity for detecting the stalk seen in stage Ta or T1. An inflammatory change or fibrosis surrounding the tumor mimics the invasion of bladder cancer on T2-weighted imaging or enhanced MRI and could lead to over-staging, but DWI could differentiate them clearly because these benign changes do not show high SI on DWI. DWI is also useful for detecting ureteral, urethral, and prostatic extension by means of the urethra. DWI provides more accurate information on the extent of bladder cancer and contributes to determination of the treatment strategy.
  • Prophylactic effects of Bacille Calmette-Guérin intravesical instillation therapy: time period-related comparison between Japan and Western countries., Okamura T, Ando R, Akita H, Kawai N, Tozawa K, Kohri K, Arano H, Current urology reports, 15, (1) ,   2013年12月, 査読有り
  • Oxygen nano-bubble water reduces calcium oxalate deposits and tubular cell injury in ethylene glycol-treated rat kidney, Yasuhiko Hirose, Takahiro Yasui, Kazumi Taguchi, Yasuhiro Fujii, Kazuhiro Niimi, Shuzo Hamamoto, Atsushi Okada, Yasue Kubota, Noriyasu Kawai, Yasunori Itoh, Keiichi Tozawa, Shoichi Sasaki, Kenjiro Kohri, UROLITHIASIS, 41, (4) 279 - 294,   2013年08月, 査読有り, Renal tubular cell injury induced by oxalate plays an important role in kidney stone formation. Water containing oxygen nano-bubbles (nanometer-sized bubbles generated from oxygen micro-bubbles; ONB) has anti-inflammatory effects. Therefore, we investigated the inhibitory effects of ONB water on kidney stone formation in ethylene glycol (EG)-treated rats. We divided 60 rats, aged 4 weeks, into 5 groups: control, the water-fed group; 100 % ONB, the 100 % ONB water-fed group; EG, the EG treated water-fed group; EG + 50 % ONB and EG + 100 % ONB, water containing EG and 50 % or 100 % ONB, respectively. Renal calcium oxalate (CaOx) deposition, urinary excretion of N-acetyl-beta-d-glucosaminidase (NAG), and renal expression of inflammation-related proteins, oxidative stress biomarkers, and the crystal-binding molecule hyaluronic acid were compared among the 5 groups. In the control and 100 % ONB groups, no renal CaOx deposits were detected. In the EG + 50 % ONB and EG + 100 % ONB groups, ONB water significantly decreased renal CaOx deposits, urinary NAG excretion, and renal monocyte chemoattractant protein-1, osteopontin, and hyaluronic acid expression and increased renal superoxide dismutase-1 expression compared with the EG group. ONB water substantially affected kidney stone formation in the rat kidney by reducing renal tubular cell injury. ONB water is a potential prophylactic agent for kidney stones.
  • Transurethral marking incision of the bladder neck: a helpful technique in robot-assisted laparoscopic radical prostatectomy involving post-transurethral resection of the prostate and cancers protruding into the bladder neck., Kurokawa S, Tozawa K, Umemoto Y, Yasui T, Mizuno K, Okada A, Kawai N, Hayashi Y, Kohri K, BMC urology, 13,   2013年08月, 査読有り
  • Thermotherapy using magnetic cationic liposomes powerfully suppresses prostate cancer bone metastasis in a novel rat model, Daichi Kobayashi, Noriyasu Kawai, Shinya Sato, Taku Naiki, Kenji Yamada, Takahiro Yasui, Keiichi Tozawa, Takeshi Kobayashi, Satoru Takahashi, Kenjiro Kohri, PROSTATE, 73, (9) 913 - 922,   2013年06月, 査読有り, BACKGROUND Bone metastasis is a serious problem for individuals with prostate cancer, and the effects of the anticancer drug docetaxel (DTX) are insufficient. We therefore examined the therapeutic potential of magnetic cationic liposomes (MCL) in a novel rat model that allows the evaluation of tumor immunity. The effects of MCL thermotherapy were compared with those of DTX as a conventional therapy for the treatment of bone metastatic prostate cancer. METHODS Prostate tumor tissues were transplanted into the femurs of model rats divided into four groups: control, MCL, DTX, and MCL+DTX. Tumors were injected with MCL, and alternating magnetic field (AMF) irradiation was performed three times a week. Tumor proliferation and bone destruction were evaluated by proliferating cell nuclear antigen positivity, computed tomography, and CD68-positive cell number, while tumor immunity was evaluated by heat shock protein (HSP) 70 expression and CD8-positive lymphocyte number. RESULTS We successfully established a novel femur metastasis model of prostate cancer, and demonstrated that tumor proliferation and bone destruction in the MCL and MCL+DTX groups were significantly suppressed compared with control and DTX groups. MCL thermotherapy concurrently induced necrosis and apoptosis. The expression of HSP70 in the MCL and MCL+DTX groups was also significantly increased, and tumor immunity was enhanced through the induction of CD8-positive lymphocytes. CONCLUSION MCL thermotherapy was clearly more effective than DTX in treating bone metastatic prostate cancer. A combination of MCL thermotherapy and DTX therefore deserves consideration as a novel treatment for this disease. Prostate 73: 913922, 2013. (c) 2013 Wiley Periodicals, Inc.
  • Neoadjuvant hormonal therapy is a feasible option in laparoscopic radical prostatectomy, Taku Naiki, Noriyasu Kawai, Takehiko Okamura, Daisuke Nagata, Yoshiyuki Kojima, Hidetoshi Akita, Takahiro Yasui, Keiichi Tozawa, Kenjiro Kohri, BMC UROLOGY, 12,   2012年12月, 査読有り, Background: Few reports can be found in the literature with respect to the impact of neoadjuvant hormonal therapy (NHT) on operative parameters on laparoscopic radical prostatectomy (LRP) in a large study. The aim of this study was to evaluate the safety and efficacy of NHT prior to LRP for locally confined prostate cancer. Methods: From January 2004 to September 2009, 342 patients undergoing LRP were analyzed, specifically comparing 72 patients who received NHT to 270 who did not. All patients were in clinical stage T2 and nerve sparing LRP were not included. Results: The mean patient age, preoperative prostate specific antigen (PSA), clinical stage, and biopsy Gleason grade were similar for the NHT and the non-NHT LRP groups. The median blood loss and the median operative time were also similar. There were no differences in the intraoperative complication rate of rectum injury, blood transfusion, and open surgery conversion. The positive surgical margin rate was significantly improved in NHT patients. Moreover, PSA recurrence within two years was significantly less in long-term NHT than in non-NHT patients. Conclusions: LRP was shown as a safe and efficacious procedure in patients who have received NHT. Perioperative morbidity of NHT patients undergoing LRP appears equivalent to non-NHT patients, with lower positive surgical margin, and PSA recurrence rate.
  • Single monthly bacillus Calmette-Guérin intravesical instillation is effective maintenance therapy to prevent recurrence in Japanese patients with non-muscle-invasive bladder cancer., Okamura T, Akita H, Ando R, Ikegami Y, Naiki T, Kawai N, Tozawa K, Kohri K, International journal of clinical oncology, 17, (5) 477 - 481,   2012年10月, 査読有り
  • Clinicopathologic Significance of High Signal Intensity on Diffusion-weighted MR Imaging in the Ureter, Urethra, Prostate and Bone of Patients with Bladder Cancer, Mitsuru Takeuchi, Tomohiro Suzuki, Shigeru Sasaki, Masato Ito, Shuzo Hamamoto, Noriyasu Kawai, Kenjiro Kohri, Masaki Hara, Yuta Shibamoto, ACADEMIC RADIOLOGY, 19, (7) 827 - 833,   2012年07月, 査読有り, Rationale and Objectives: The aim of this study was to determine the clinicopathologic significance of high-intensity areas in the ureter, urethra, prostate, and bone incidentally found on diffusion-weighted magnetic resonance imaging (DWI) for the staging of bladder cancer. Materials and Methods: Axial and sagittal DWI and T2-weighted imaging of the pelvis were evaluated in 157 patients with bladder cancer. Two observers assessed T2-weighted imaging with DWI independently. The observers pointed out 67 areas showing abnormal high signal intensity on DWI in the ureter (n = 17), urethra (n = 8), prostate (n = 20), and bone (n = 22). Of the 67 high-intensity areas, 33 lesions were confirmed histopathologically (ureter, n = 10; urethra, n = 7; prostate, n = 16), and 22 bone lesions were diagnosed using T1-weighted imaging and follow-up computed tomography. Thus, 55 lesions were evaluable for correlation with DWI findings. Results: Of the 55 high-intensity areas, 28 (53%) were synchronous or metastatic urothelial cancer or invasion of urothelial cancer. The remaining 27 (47%) were a ureteral clot in one, a ureteral stone granuloma in one, prostatic cancer in six, granulomatous prostatitis in three, and normal red bone marrow in 16. Conclusions: DWI is useful to comprehend the extent of bladder cancer and to detect incidentally coexisting diseases. Other imaging, endoscopic, and clinical findings would be useful to reduce false positivity.
  • Unilateral testicular seminoma with simultaneous contralateral torsion: a case report., Taguchil K, Yasui T, Naiki T, Umemoto Y, Kojima Y, Kawai N, Tozawa K, Hayashi Y, Kohri K, Journal of medical case reports, 6,   2012年07月, 査読有り
  • Pelvic solitary fibrous tumor originally diagnosed as prostatic in origin, Ryosuke Ando, Daichi Kobayashi, Taku Naiki, Noriyasu Kawai, Shoichi Sasaki, Kenjiro Kohri, CLINICAL IMAGING, 36, (3) 243 - 245,   2012年05月, 査読有り, A 71-year-old man was referred to our hospital because of intermittent urine stream and postmicturition dribbling. Magnetic resonance imaging (MRI) results suggested the mass to be a malignant mesenchymal tumor arising from the left lobe of the prostate, on the basis of the presence of a beak sign. Radical prostatectomy and partial rectal excision with subsequent colostomy were performed. Contrary to preoperative MRI, no prostate involvement was found on histologic examination. Histopathologic and immunohistochemical findings showed typical characteristics of solitary fibrous tumors. The patient's postoperative course was uneventful. He showed no signs of recurrence and metastasis at 2-year follow-up. (C) 2012 Elsevier Inc. All rights reserved.
  • Successful treatment of primary malignant lymphoma of the penis by organ-preserving rituximab-containing chemotherapy, Shuzo Hamamoto, Keiichi Tozawa, Hideki Nishio, Noriyasu Kawai, Kenjiro Kohri, INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY, 17, (2) 181 - 184,   2012年04月, 査読有り, Primary penile lymphoma is extremely rare. Here we report the case of a 67-year-old man with the chief complaints of difficulty in urination and priapism, who was eventually diagnosed with primary malignant lymphoma of the penis. Pathological examination of excision biopsy of the left inguinal lymph node revealed malignant CD20+ diffuse large B-cell lymphoma. We treated this patient with a systemic rituximab-chemotherapy regimen and obtained good results, in terms of both functional and cosmetic outcomes. Soluble interleukin-2 receptor was a useful tumor marker for evaluating the therapeutic effect. The patient has been in remission for 10 months after the discontinuation of chemotherapy.
  • Are there Time-period-related Differences in the Prophylactic Effects of Bacille Calmette-Guerin Intravesical Instillation Therapy in Japan?, Takehiko Okamura, Ryosuke Ando, Hidetoshi Akita, Yoshihiro Hashimoto, Yutaka Iwase, Taku Naiki, Noriyasu Kawai, Keiichi Tozawa, Kenjiro Kohri, ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 13, (9) 4357 - 4361,   2012年, 査読有り, Objective: The guidelines on indications for prophylactic use of Bacille Calmette-Guerin (BCG) against non-muscle-invasive bladder cancer (NMIBC) have changed over the years. In order to assess the impact on outcome, the present retrospective comparison of BCG efficacy by time period with Japanese patients was conducted. Patients and Methods: A total of 146 cases of NMIBC treated with BCG since February 1985 were retrospectively evaluated. All patients received 80 mg of BCG (Tokyo 172 strain) six to eight times a week for prophylactic use. Comparison was made among three historical groups (Group A: 1980's, 39 cases; Group B: 1990's, 61 cases; Group C: 2000's, 46 cases). Results: In total, recurrence was seen in 55 of the 146 cases (37.7%), and progression in 14 (9.6%), 1 patient dying of cancer. These overall results were similar to those outlined in previous reports. However, the outcomes of this time-period-based analysis indicated a tendency for a shorter time to recurrence in patients after 2000, although a log-rank test showed no significance (P=0.229). Seven of the cases featuring progression (i.e., half of all such cases) were among the 46 Group C patients (15.2%). Excluding these progressive cases, there was no significant difference among the remaining 132 patients in the three groups. Conclusion: This study results revealed a tendency for a lower non-recurrence rate after 2000 in our series. This could stem from a number of factors, including changes in BCG indication criteria and the evolution of histopathological diagnostic criteria.
  • Are there Time-period-related Differences in the Prophylactic Effects of Bacille Calmette-Guerin Intravesical Instillation Therapy in Japan?, Takehiko Okamura, Ryosuke Ando, Hidetoshi Akita, Yoshihiro Hashimoto, Yutaka Iwase, Taku Naiki, Noriyasu Kawai, Keiichi Tozawa, Kenjiro Kohri, ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 13, (9) 4357 - 4361,   2012年, 査読有り, Objective: The guidelines on indications for prophylactic use of Bacille Calmette-Guerin (BCG) against non-muscle-invasive bladder cancer (NMIBC) have changed over the years. In order to assess the impact on outcome, the present retrospective comparison of BCG efficacy by time period with Japanese patients was conducted. Patients and Methods: A total of 146 cases of NMIBC treated with BCG since February 1985 were retrospectively evaluated. All patients received 80 mg of BCG (Tokyo 172 strain) six to eight times a week for prophylactic use. Comparison was made among three historical groups (Group A: 1980's, 39 cases; Group B: 1990's, 61 cases; Group C: 2000's, 46 cases). Results: In total, recurrence was seen in 55 of the 146 cases (37.7%), and progression in 14 (9.6%), 1 patient dying of cancer. These overall results were similar to those outlined in previous reports. However, the outcomes of this time-period-based analysis indicated a tendency for a shorter time to recurrence in patients after 2000, although a log-rank test showed no significance (P=0.229). Seven of the cases featuring progression (i.e., half of all such cases) were among the 46 Group C patients (15.2%). Excluding these progressive cases, there was no significant difference among the remaining 132 patients in the three groups. Conclusion: This study results revealed a tendency for a lower non-recurrence rate after 2000 in our series. This could stem from a number of factors, including changes in BCG indication criteria and the evolution of histopathological diagnostic criteria.
  • Preoperative Prediction of Neurovascular Bundle Involvement of Localized Prostate Cancer by Combined T2 and Diffusion-weighted Imaging of Magnetic Resonance Imaging, Number of Positive Biopsy Cores, and Gleason Score, Taku Naiki, Takehiko Okamura, Daisuke Nagata, Yuji Mori, Noriyasu Kawai, Kumiko Ogawa, Hidetoshi Akita, Yoshihiro Hashimoto, Keiichi Tozawa, Kenjiro Kohri, ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 12, (4) 909 - 913,   2011年, 査読有り, Because recovery of erectile function and avoidance of positive surgical margins are important but competing outcomes with prostate cancer therapy, the decision to preserve or resect a neurovascular bundle (NVB) during laparoscopic radical prostatectomy (LRP) should be firmly based on information concerning the presence and location of extracapsular extension. In the current retrospective study, the propriety of actual decisions was assessed using preoperative magnetic resonance imaging (MRI), combining T2-weighted imaging (T2WI) with diffusion-weighted imaging (DWI), the apparent diffusion coefficient (ADC), numbers of positive biopsy cores, tumor volume and the Gleason score. MRI before prostate biopsy was performed in 35 patients who underwent LRP for clinically localized prostate cancer. A single radiologist retrospectively assessed whether the tumor localization, capsular penetration, seminal vesicle invasion, NVB involvement, and MRI findings correlated with the postoperative histological results. With the postoperative specimens, 83 lesions demonstrated a Gleason score of 6 or more. Using T2WI with and without DWI and ADC, 39 and 27 of 54 lesions were correctly identified, respectively, the difference being significant. For cancers in the transitional zone, using a threshold Gleason score of 3 or greater, sensitivity was also significantly higher for T2+DWI+ADC than for T2WI alone. Of 35 patients, using all available clinical information (biopsy results including Gleason score, tumor location, percentage of positive biopsy cores, and the percentage of tumor-involved core tissue), we found that the preoperative and postoperative staging were concordant in 25 cases. There is no universal consensus for nerve-sparing LRP; therefore, we performed an additional analysis using simplified clinically defined selection criteria (PSA level >15ng/mL, cT2, less than two positive biopsy scores in the unilateral lobe and less than 30% tumor volume, and a Gleason score of 6). Using this criteria, we selected 12 of 35 patients, and the detection rate of NVB involvement by MRI combined T2WI + DWI + ADC maps was 100% in their 30 lesions, and therefore we consider it safe to perform nerve-sparing LRP using our criteria. Our findings suggest that NVB can be safely preserved in patients with low-grade tumors using simplified clinically defined selection criteria to determine margin involvement.
  • Giant Retroperitoneal Mucinous Tumor Supportively Diagnosed as a Dedifferentiated Liposarcoma by Fluorescence In Situ Hybridization of MDM2 Gene., Naiki T, Hamamoto S, Kawai N, Naiki-Ito A, Kojima Y, Yasui T, Tozawa K, Kohri K, ISRN urology, 2011,   2011年, 査読有り
  • Advantages of Second Line Estramustine for Overall Survival of Hormone-Refractory Prostate Cancer (HRPC) Patients, Taku Naiki, Takehiko Okamura, Noriyasu Kawai, Hiroshi Sakagami, Yasuyuki Yamada, Keiji Fujita, Hidetoshi Akita, Yoshihiro Hashimoto, Keiichi Tozawa, Kenjiro Kohri, ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 10, (1) 71 - 74,   2009年, 査読有り, There is no effective standard therapy for the treatment of hormone refractory prostate cancer (HRPC), and treatments vary among different medical institutions with efforts to improve results. The present retrospective investigation was performed to assess the outcomes of second line, third line, and fourth line therapies. A total of 142 patients with HRPC were treated at Nagoya City University Hospital and its affiliate hospitals during the 10 years between October 1996 and August 2006. Patient background and treatments given after hormone refractory phase were determined, with especial attention to 50% or greater decrease rates of serum PSA levels and other variables with three common regimens based on: estramustine phosphate (EMP); diethylstilbestrol diphosphate (DES); and dexamethasone (DEX). With second line therapy for HRPC, the response rate was highest with EMP, whereas best outcomes were apparent with DES as a third line or fourth line therapy. However, overall survival for all cases and particularly with those having a poorly differentiated lesion, was best with EMP in any time period. Although there is no generally established optimal treatment for HRPC, our analysis supports the efficacy of EMP based on second line therapy response rates and optimal prognosis with longer term use.
  • Malignant mesothelioma of the tunica vaginalis testis related to recent asbestos., Ikegami Y, Kawai N, Tozawa K, Hayashi Y, Kohri K, International journal of urology : official journal of the Japanese Urological Association, 15, (6) 560 - 561,   2008年06月, 査読有り
  • Effect of heat therapy using magnetic nanoparticles conjugated with cationic liposomes on prostate tumor in bone, Kawai N, Futakuchi M, Yoshida T, Ito A, Sato S, Naiki T, Honda H, Shirai T, Kohri K, Prostate, 68, (7) 784 - 792,   2008年05月, 査読有り
  • Evaluation of operative complications related to laparoscopic radical prostatectomy., Tozawa K, Hashimoto Y, Yasui T, Itoh Y, Nagata D, Akita H, Kawai N, Hayashi Y, Kohri K, International journal of urology : official journal of the Japanese Urological Association, 15, (3) 222 - 225,   2008年03月, 査読有り
  • Complete regression of experimental prostate cancer in nude mice by repeated hyperthermia using magnetite cationic liposomes and a newly developed solenoid containing a ferrite core, N Kawai, A Ito, Y Nakahara, H Honda, T Kobayashi, M Futakuchi, T Shirai, K Tozawa, K Kohri, PROSTATE, 66, (7) 718 - 727,   2006年05月, 査読有り, BACKGROUND. Magnetite cationic liposomes (MCLs) can be used to induce hyperthermia because they generate heat in an alternating magnetic field (AMF). This study aimed at developing more practical method for MCL hyperthermia examining the effect of MCL-induced hyperthermia on human prostate cancer in vivo. MATERIALS AND METHODS. A newly developed AMF generator incorporating a solenoid with a ferrite core (FC) was used. Human prostate cancer cells (PC-3 and LNCap) were injected subcutaneously into nude mice. MCLs were injected into tumor nodule and the mice were exposed into AMF three times at 24-hr intervals (repeated hyperthermia; RH) until complete tumor regression was observed. RESULTS. Irradiation with an AMF generated by newly developed device can adequately increase the temperature of tumor tissue. Frequent RH resulted in complete tumor regression in all nude mice. CONCLUSION. RH using MCLs may be a promising new therapy for hormone-refractory human prostate cancer in the future.
  • Long-term outcome of upper urinary tract carcinoma in situ: effectiveness of nephroureterectomy versus bacillus Calmette-Guérin therapy., Kojima Y, Tozawa K, Kawai N, Sasaki S, Hayashi Y, Kohri K, International journal of urology : official journal of the Japanese Urological Association, 13, (4) 340 - 344,   2006年04月, 査読有り
  • Anticancer effect of hyperthermia on prostate cancer mediated by magnetite cationic liposomes and immune-response induction in transplanted syngeneic rats, N Kawai, A Ito, Y Nakahara, M Futakuchi, T Shirai, H Honda, T Kobayashi, K Kohri, PROSTATE, 64, (4) 373 - 381,   2005年09月, 査読有り, BACKGROUND. The hyperthermic effect of magnetic particles was examined in rat prostate cancer in vivo. Magnetic cationic liposomes (MCLs) have a positive surface charge and generate heat in an alternating magnetic field (AMF) due to hysteresis losses. METHODS. Rat prostate cancer cells (PLS 10; androgen independent) were injected subcutaneously into the flank of F344 rats. MCLs were injected into rat prostate cancer nodules that had grown to 5-6 mm in diameter, and were then exposed to an AMF. Tumor growth rates were measured. To examine whether hyperthermia caused immune induction for PLS 10, cytotoxicity assays and immunohistochemical staining for CD3, CD4, CD8, and Heat Shock Protein (HSP) 70 were performed. RESULT. The tumor temperature increased to 45 degrees C whereas the body temperature remained at around 38 degrees C. Tumor regression was observed in the hyperthermic group. CD3, CD4, and CD8 immunocytes were present in the tumor tissues of the rats exposed to hyperthermia, but they were not detected in any of the tumor tissue of untreated rats. HSP70 also appeared in the viable area at its boundary with the necrotic area. The cytotoxic activity of tumor-transplanted rats for PLS 10 cells increased in hyperthermic-treatment rats. CONCLUSION. These results suggest that hyperthermia using MCLs is an effective therapy for prostate cancer, since this treatment appears to kill the prostate cancer cells not only directly by heating but also by inducing an immune response. This therapy may cure not only the primary lesion but also metastatic lesions.
  • MMP-7 promotes prostate cancer-induced osteolysis via the solubilization of RANKL, CC Lynch, A Hikosaka, HB Acuff, MD Martin, N Kawai, RK Singh, TC Vargo-Gogola, JL Begtrup, TE Peterson, B Fingleton, T Shirai, LM Matrisian, M Futakuchi, CANCER CELL, 7, (5) 485 - 496,   2005年05月, 査読有り, We developed a rodent model that mimics the osteoblastic and osteolytic changes associated with human metastatic prostate cancer. Microarray analysis identified MMP-7, cathepsin-K, and apolipoprotein D as being upregulated at the tumor-bone interface. MMP-7, which was produced by osteoclasts at the tumor-bone interface, was capable of processing RANKL to a soluble form that promoted osteoclast activation. MMP-7-cleficient mice demonstrated reduced prostate tumor-induced osteolysis and RANKL processing. This study suggests that inhibition of MMP-7 will have therapeutic benefit in the treatment of prostate cancer-induced osteolysis.
  • Positive correlation between sialyl Lewis X expression and pathologic findings in renal cell carcinoma, K Tozawa, T Okamoto, N Kawai, Y Hashimoto, Y Hayashi, K Kohri, KIDNEY INTERNATIONAL, 67, (4) 1391 - 1396,   2005年04月, 査読有り, Background. Interaction between tumor cells and endothelium plays a major role in cancer invasion and metastasis. Among various cell adhesion molecules, the cognate interaction between sialyl Lewis antigen expressed in the tumor cell surface and E-selectin expressed on endothelial cells is considered to be crucial for the tumor cell adhesion to the endothelium. Methods. The sialyl Lewis X (sL(X)) expression in 45 specimens from renal cell carcinoma patients was examined using immunohistochemistry. Results. In this study, we demonstrate that the immunoreactivity for sL(X) in renal cell carcinoma specimens not only correlates with conventional histopathologic parameters but also serves as a useful indicator for the prognosis of renal cell carcinoma. Conclusion. Since beneficial effect of cimetidine has been reported and ascribed to its inhibitory action on the expression of E-selectin, a ligand molecule of sialyl Lewis antigen, cimetidine may also show inhibitory effect on the tumor recurrence and metastasis of renal cell carcinoma with high level of sL(X) expression.
  • Intravenous urography-virtual cystoscopy is a better preliminary examination that air virtual cystoscopy, N Kawai, T Mimura, D Nagata, K Tozawa, K Kohri, BJU INTERNATIONAL, 94, (6) 832 - 836,   2004年10月, 査読有り, OBJECTIVE To describe a new technique of virtual cystoscopy (VC, used previously but with catheterization to drain residual urine and insufflation with air or carbon dioxide) with no invasive catheterization, used in parallel with intravenous urography (IVU), as conventional cystoscopy is an invasive but essential examination, and VC with multislice computed tomography (CT) was introduced to make preliminary examinations noninvasive. PATIENTS AND METHODS Using multislice CT and a device with 16 rows of detectors, we examined five patients using VC that previously involved catheterization, termed 'air VC' and 16 using VC with the new technique, termed 'IVU VC' We assessed the new technique by evaluating the tumour detection rate, and merits and demerits of both types of VC. RESULTS The detection rate of bladder tumours by IVU VC was similar to that from air VC; moreover, IVU VC overcame two significant disadvantages of air VC, i.e. the appearance of the water surface and the need for catheterization. CONCLUSION Conventional cystoscopy is still an essential examination but this new method of IVU VC may be ideal for preliminary examination of the bladder.
  • Bacillus Calmette-Guerin-refractory superficial bladder cancers: focus on pretreatment episodes., Okamura T, Akita H, Tozawa K, Kawai N, Nagata D, Kohri K, International journal of clinical oncology, 8, (3) 168 - 173,   2003年06月, 査読有り
  • Gold compounds inhibit adhesion of human cancer cells to vascular endothelial cells, K Tozawa, N Kawai, Y Hayashi, S Sasaki, K Kohri, T Okamoto, CANCER LETTERS, 196, (1) 93 - 100,   2003年06月, 査読有り, Transcription factor NF-kappaB controls the expression of a number of genes including those for cell adhesion molecules such as E-selectin, ICAM-1 and VCAM-1. These cell adhesion molecules are known to play important roles in a critical step of tumor metastasis; the arrest of tumor cells on the venous or capillary bed of the target organ. NF-kappaB is activated by extracellular signals such as those elicited by the proinflammatory cytokines, TNF and IL-1. Here we demonstrate that IL-1beta induces nuclear translocation of NF-kappaB in human umbilical vein endothelial cells (HUVEC) followed by induction of cell surface expression of E-selectin, ICAM-1 and VCAM-1, and subsequently augments adhesion of cancer cells expressing sialyl Lewis antigen, a ligand of E-selectin. We also demonstrated that the adhesion of tumor cells to IL-1beta-treated HUVEC was inhibited by gold compounds such as aurothioglucose and aurothiornalate. These observations indicate the involvement of NF-kappaB in cancer metastasis and suggest the feasibility of using gold compounds to prevent metastasis. (C) 2003 Elsevier Science Ltd. All rights reserved.
  • Gold compounds inhibit adhesion of human cancer cells to vascular endothelial cells, K Tozawa, N Kawai, Y Hayashi, S Sasaki, K Kohri, T Okamoto, CANCER LETTERS, 196, (1) 93 - 100,   2003年06月, 査読有り, Transcription factor NF-kappaB controls the expression of a number of genes including those for cell adhesion molecules such as E-selectin, ICAM-1 and VCAM-1. These cell adhesion molecules are known to play important roles in a critical step of tumor metastasis; the arrest of tumor cells on the venous or capillary bed of the target organ. NF-kappaB is activated by extracellular signals such as those elicited by the proinflammatory cytokines, TNF and IL-1. Here we demonstrate that IL-1beta induces nuclear translocation of NF-kappaB in human umbilical vein endothelial cells (HUVEC) followed by induction of cell surface expression of E-selectin, ICAM-1 and VCAM-1, and subsequently augments adhesion of cancer cells expressing sialyl Lewis antigen, a ligand of E-selectin. We also demonstrated that the adhesion of tumor cells to IL-1beta-treated HUVEC was inhibited by gold compounds such as aurothioglucose and aurothiornalate. These observations indicate the involvement of NF-kappaB in cancer metastasis and suggest the feasibility of using gold compounds to prevent metastasis. (C) 2003 Elsevier Science Ltd. All rights reserved.
  • [Clinical evaluation of ureteral endometriosis: report of 8 cases]., Nishihara K, Kawai N, Hibino M, Tozawa K, Sasaki S, Hayashi Y, Kohri K, Hinyokika kiyo. Acta urologica Japonica, 49, (4) 185 - 187,   2003年04月, 査読有り
  • The efficacy of a serum carboxyterminal pyridinoline cross-linked telopeptide of type I collagen as a quantitative screening marker for bone metastases in patients with urological malignancies, T Okamura, H Akita, H Tatsura, N Kawai, D Nagata, M Azemoto, Y Iwase, K Kohri, CANCER DETECTION AND PREVENTION, 27, (1) 14 - 18,   2003年, 査読有り, In order to ascertain whether carboxyterminal pyridinoline cross-linked telopeptide of type I collagen (ICTP) might be useful as a serum screening parameter for bone metastases from non-prostate urological malignancies as well as prostate cancers, as series of 210 patients were examined. In addition to ICTP, serum alkaline phosphatase (ALP) and also prostate specific antigen (PSA) in the prostate cancer cases were assayed using commercial kits. The areas under the receiver operating characteristic (ROC) curves were 0.7846 for ICTP (cut-off point 9.6 mug/l), 0.8304 for ALP in prostate cancer cases, and 0.8278 for ICTP (cut-off point 10.6 mug/l), and 0.7139 for ALP in non-prostate cancer cases. While significance was only observed for ICTP and PSA in prostate cancer cases, borderline significance was also evident with ICTP for non-prostate malignancies, and with ALP for prostate cancer case. The results suggest that serum ICTP may be useful in combination with ALP as a quantitative clinical marker for low cost screening for bone metastases in patients with all types of urological malignancies. (C) 2002 International Society for Preventive Oncology. Published by Elsevier Science Ltd. All rights reserved.
  • The efficacy of a serum carboxyterminal pyridinoline cross-linked telopeptide of type I collagen as a quantitative screening marker for bone metastases in patients with urological malignancies, T Okamura, H Akita, H Tatsura, N Kawai, D Nagata, M Azemoto, Y Iwase, K Kohri, CANCER DETECTION AND PREVENTION, 27, (1) 14 - 18,   2003年, 査読有り, In order to ascertain whether carboxyterminal pyridinoline cross-linked telopeptide of type I collagen (ICTP) might be useful as a serum screening parameter for bone metastases from non-prostate urological malignancies as well as prostate cancers, as series of 210 patients were examined. In addition to ICTP, serum alkaline phosphatase (ALP) and also prostate specific antigen (PSA) in the prostate cancer cases were assayed using commercial kits. The areas under the receiver operating characteristic (ROC) curves were 0.7846 for ICTP (cut-off point 9.6 mug/l), 0.8304 for ALP in prostate cancer cases, and 0.8278 for ICTP (cut-off point 10.6 mug/l), and 0.7139 for ALP in non-prostate cancer cases. While significance was only observed for ICTP and PSA in prostate cancer cases, borderline significance was also evident with ICTP for non-prostate malignancies, and with ALP for prostate cancer case. The results suggest that serum ICTP may be useful in combination with ALP as a quantitative clinical marker for low cost screening for bone metastases in patients with all types of urological malignancies. (C) 2002 International Society for Preventive Oncology. Published by Elsevier Science Ltd. All rights reserved.
  • N-acetyl-L-cysteine enhances chemotherapeutic effect on prostate cancer cells, K Tozawa, T Okamoto, Y Hayashi, S Sasaki, N Kawai, K Kohri, UROLOGICAL RESEARCH, 30, (1) 53 - 58,   2002年03月, 査読有り, Transcription factor nuclear factor kappaB (NF-kappaB) controls gene expression of a number of genes, including cytokines such as interleukin-6 (IL-6), granulocyte-macrophage (GM)-CSF, and interleukin-8 (IL-8). IL-6 is known to play important roles in the growth of prostate cancer cells, activation of androgen receptor, and prostate-specific protein expression. NF-kappaB is activated by extracellular signals such as proinflammatory cytokines, chemotherapeutic reagents, and radiation. Here we demonstrate that cisplatin (CDDP) and etoposide (VP-16) induce nuclear translocation of NF-kappaB in prostate cancer cell lines, followed by secretion of IL-6. We also demonstrated that the growth of hormone-independent prostate cancer cell lines can be inhibited by the anti-NF-kappaB reagent N-acetyl-L-cysteine (NAC). These observations indicate that NF-kappaB can be a target of new adjuvant therapy against hormone refractory prostate cancer.

競争的資金

  • IVR技術で磁性微粒子を癌転移巣に注入する新規温熱治療の臨床応用に向けた基礎研究, 名古屋市立大学,   2015年04月01日 - 2018年03月31日
  • 細胞間輸送機構を標的としたホルモン療法抵抗性前立腺癌治療法確立に向けた基礎的研究, 名古屋市立大学,   2014年04月01日 - 2017年03月31日
  • ポリエチレングリコールによる多核細胞形成を介した膀胱腫瘍増殖抑制効果の検討, 名古屋市立大学,   2013年04月01日 - 2016年03月31日
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  • 磁場誘導組織内加温法と免疫治療の融合による前立腺癌に対する新しい治療法の開発,   2012年04月01日 - 2015年03月31日
  • ホルモン耐性前立腺癌に対する核内受容体転写共役機構の解析と新規分子標的治療の開発,   2012年04月01日 - 2015年03月31日


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