KAGAMI Hiroyuki

Researcher Information

Degree

• Ph. D.

J-Global ID

• 200901036068734943

Research Areas

• Natural sciences / Mathematical physics and basic theory

Academic & Professional Experience

• 2022/04 - Today  周南公立大学福祉情報学部教授
• 2020/04 - 2022/03  Tokuyama UniversityFaculty of Welfare and InformationProfessor
• 2018/10 - 2020/03  Fujita Health UniversitySchool of Medicine准教授
• 2014/08 - 2018/10  藤田保健衛生大学医学部准教授
• 2007/04 - 2014/07  Nagoya College准教授
• 2006/04 - 2014/07  Nagoya CollegeAssociate Professor
• 2006/04 - 2007/03  Nagoya College助教授
• 2003/04 - 2006/03  Nagoya CollegeLecturer
• 2003/04 - 2006/03  Nagoya College講師
• 2002/04 - 2003/03  Wakkanai Hokusei Gakuen CollegeResearch Associate
• 2002/04 - 2003/03  Wakkanai Hokusei Gakuen CollegeFaculty of Integrated Media助手

Education

• 1996/04 - 2002/03  京都大学大学院  理学研究科  物理学・宇宙物理学専攻
• 1996 - 2002  Kyoto University  Graduate School of Science  Division of Physics and Astronomy
• 1994/04 - 1996/03  京都大学大学院  理学研究科  地球惑星科学専攻
• 1994 - 1996  Kyoto University  Graduate School of Science  Department of Geophysics
• 1991/04 - 1993/03  The University of Tokyo  Faculty of Science  Department of Astronomy
• 1991 - 1993  The University of Tokyo  Faculty of Science  Department of Astronomy
• 1989/04 - 1991/03  The University of Tokyo  College of Arts and Sciences  理科一類
• 1989 - 1991  The University of Tokyo  College of Arts and Sciences  Natural Sciences I

Association Memberships

• 異文化間情報連携学会   応用物理学会   日本温泉科学会   日本物理学会   情報処理学会   

Published Papers

• Direction of Regional Tourism atfer COVID-19

  寺田篤史; 鏡裕行; 中嶋克成; 羽田司

  徳山大学総合研究所紀要 44 45 - 54 2022/03
• Fostering Fundamental Competencies Among Prospective Healthcare Professionals Through Engaging in Study-Support for Children with Foreign Backgrounds

  Shinobu Hattori; Hiroyuki Kagami

  徳山大学総合研究所紀要 44 63 - 72 2022/03
• Study of the eruption mechanism of Saturn's moon Enceladus plume using the mathematical model of a geyser (periodic bubbling spring)

  Hiroyuki Kagami

  12th IEEE Integrated STEM Education Conference (in press) 2022/03 [Refereed]
  
• The Modified Mathematical Model of Arteriosclerosis onset: Adding the Effect of Repairing Flaws of the Intima

  Hiroyuki Kagami

  2021 12th International Symposium on Advanced Topics in Electrical Engineering (ATEE) IEEE 2021/03 [Refereed]
  
• The Modified Mathematical Model of the Pathogenesis of Urolithiasis: Add Calculi Dissolution Effect

  Hiroyuki Kagami

  (The conference proceedings of) 2020 3rd International Conference on Computer Applications & Information Security (ICCAIS) IEEE 2020/05 [Refereed]
  
• Evaluation of progression of the symptoms based on the mathematical model of arteriosclerosis onset and measures to control it

  Hiroyuki Kagami

  2019 IEEE 1st Sustainable Cities Latin America Conference (SCLA) IEEE 2019/08 [Refereed]
  
• The conference proceedings of the 22nd IEEE International Conference on Computational Science and Engineering

  Hiroyuki Kagami

  The conference proceedings of the 22nd IEEE International Conference on Computational Science and Engineering 233 - 236 2019/08 [Refereed]
  
• A mathematical model of arteriosclerosis onset

  Hiroyuki Kagami

  (The conference proceedings of) 2019 Advances in Science and Engineering Technology International Conferences 2019/05 [Refereed]
  
• A mathematical model of spray drying granulation process in formulation

  Hiroyuki Kagami

  21st International Drying Symposium Proceedings 99 - 105 2018/09 [Refereed]
  
• Suppression of calculi growth based on the mathematical model of the pathogenesis of urolithiasis

  Hiroyuki Kagami

  Proceedings - 2018 International Conference on Advanced Communication Technologies and Networking, CommNet 2018 Institute of Electrical and Electronics Engineers Inc. 1 - 3 2018/05 [Refereed]
  
• Reproduction of the Transition between Spouting and Pause by the Dynamical Model of a Geyser Induced by Gas Inflow with Consideration of Influx of Groundwater

  Hiroyuki Kagami

  Proceedings of the International Conference of Recent Trends in Environmental Science and Engineering 113-1 - 113-2 2017/08 [Refereed]
  
• Further improvement of the unevenness of the edge portion of the coating film after drying by controlling the evaporation rate of the edge's side surface of the coating liquid film during drying

  Hiroyuki Kagami

  Functional Nanostructures Proceedings 1 (2) 58 - 60 2017/08 [Refereed]
  
• The mathematical model that describes the periodic spouting of a geyser induced by boiling

  Hiroyuki Kagami

  Proc. SPIE SPIE-INT SOC OPTICAL ENGINEERING 10169 101692K-1 - 101692K-7 0277-786X 2017/04 [Refereed]
  
• Dependence on Latent Heat of Vaporization of Locally Thicker Thin Film Formation by Partial Heating of a Polymer Solution Film on the Substrate from the Bottom during Drying

  Kagami Hiroyuki

  Nano Energy Systems 1 (1) 17 - 21 2017/03 [Refereed]
  
• The Mathematical Model of the Pathogenesis of Urolithiasis

  Kagami Hiroyuki

  2nd International Conference on Science, Ecology and Technology - 2016 PROCEEDING BOOK 774 - 777 2016/11 [Refereed]
  
• Application of the dynamical model of a geyser induced by gas inflow to the changes in water level of the Shinyu hot spring pond in Tateyama Caldera

  Kagami H; K. Fukui

  Journal of Hot Spring Sciences 66 (1) 40 - 45 2016 [Refereed]
  
• 立山カルデラ内の火口湖「新湯」の水位変動への間欠泡沸泉の噴出動力学モデルの適用

  鏡裕行; 福井幸太郎

  温泉科学 66 (1) 40 - 45 2016 [Refereed]
  
• Mechanism of Locally Thicker Thin Film Formation by Partial Heating of a Polymer Solution Film on the Substrate from the Bottom during Drying

  Kagami Hiroyuki

  The Ninth International Conference on Thermal Engineering Theory and Applications (Proceedings) 2016 [Refereed]
  
• A model of drying process of a polymer liquid film coated on three-dimensional structure

  Kagami Hiroyuki

  Poceedings of 20th International Drying Symposium A-1-4  2016 [Refereed]
  
• A dynamical model of a geyser induced by gas inflow

  Kagami Hiroyuki

  Journal of Hot Spring Sciences 日本温泉科学会 65 (4) 234 - 245 0030-2821 2016 [Refereed]
  
• Protection of geysers as tourism and environmental resources : Applications of the dynamical model of a geyser induced by gas inflow

  Hiroyuki Kagami

  Proceedings of World Engineering Conference and Convention 2015 2015
• Control of the Marangoni Effect in the Drying Process of a Polymer Solution Coated on a Flat Substrate through Temperature, Evaporation and Solute Concentration Management

  Kagami Hiroyuki

  Athens Journal of Technology & Engineering 2 (1) 45 - 53 2015 [Refereed]
  
• A static model of a geyser induced by gas inflow : Understanding spouting periods through the model

  Kagami Hiroyuki

  International Journal of Geology 9 53 - 58 2015 [Refereed]
  
• More minute thickness control of a thin film after drying through temperature, evaporation and concentration management in drying process of a polymer solution coated on a flat substrate

  Kagami Hiroyuki

  Poceedings of 19th International Drying Symposium 2014 [Refereed]
  
• Impact of the Marangoni effect on the thin film thickness profile after drying polymer solution coated on a flat substrate

  Kagami Hiroyuki

  JPS Conf. Proc. 1 015087-1 - 015087-5 2014 [Refereed]
  
• Impact of the Marangoni effect on the polymer thin film thickness profile after drying polymer solution coated on a flat substrate

  Hiroyuki Kagami

  NANOENGINEERING: FABRICATION, PROPERTIES, OPTICS, AND DEVICES X SPIE-INT SOC OPTICAL ENGINEERING 8816 881606-1 - 881606-5 0277-786X 2013
• Effect of pinpoint-like heating in the drying process of a polymer solution film on a substrate

  Hiroyuki Kagamiy; Hiroshi Kubota

  e-Journal of Surface Science and Nanotechnology 10 561 - 564 1348-0391 2012/10 [Refereed]
  
• A dynamical model of a geyser induced by boiling: an application of the dynamical model of a geyser induced by inflow of gas

  Hiroyuki Kagami

  “Water resources and wetlands” Conference proceedings 55 - 58 2012
• THREE-SOURCE DYNAMICAL MODEL FOR ESTIMATING PARAMETERS FOR IRREGULARLY SPOUTING GEYSERS INDUCED BY GAS INFLOW

  Hiroyuki Kagami

  ADVANCES IN GEOSCIENCES VOL 29: HYDROLOGICAL SCIENCE (HS) WORLD SCIENTIFIC PUBL CO PTE LTD 29 145 - 156 2012 [Refereed]
  
• Thickness control of a thin film after drying through thermal and evaporative management in drying process of a polymer solution coated on a flat substrate: application of the dynamical model of the drying process

  Hiroyuki Kagami; Hiroshi Kubota

  BIOELECTRONICS, BIOMEDICAL, AND BIOINSPIRED SYSTEMS V AND NANOTECHNOLOGY V SPIE-INT SOC OPTICAL ENGINEERING 8068 806813-1 - 806813-6 0277-786X 2011
• A dynamical model of drying process of a polymer solution having plural solvents and plural solutes (polymers) coated on a flat substrate for a flat and homogeneous polymer film fabrication

  Hiroyuki Kagami; Hiroshi Kubota

  PHYSICA STATUS SOLIDI C: CURRENT TOPICS IN SOLID STATE PHYSICS, VOL 8, NO 2 WILEY-V C H VERLAG GMBH 8 (2) 586 - 588 1862-6351 2011 [Refereed]
  
• An extended dynamical model of a geyser induced by inflow of gas in case of plural underground gas supply sources (2)

  Kagami Hiroyuki

  Mathematical Modelling in Civil Engineering 7 (2) 29 - 34 2011 [Refereed]
  
• A modified dynamical model of drying process of a polymer solution having plural solvents coated on a flat substrate for a flat and homogeneous polymer film fabrication

  Hiroyuki Kagami

  MICRO-OPTICS 2010 SPIE-INT SOC OPTICAL ENGINEERING 7716 771625-1 - 771625-9 0277-786X 2010
• Applying the dynamical model of drying process of a polymer solution coated on a flat substrate to effects of bumpy substrate

  Hiroyuki Kagami; Hiroshi Kubota

  NANOENGINEERING: FABRICATION, PROPERTIES, OPTICS, AND DEVICES VII SPIE-INT SOC OPTICAL ENGINEERING 7764 77640T-1 - 77640T-8 0277-786X 2010
• Thickness control of a thin film after drying through thermal management in drying process of a polymer solution coated on a flat substrate : application of the dynamical model of the drying process

  H. Kagami; H. Kubota

  Proceedings of the 2nd International Symposium on Thermal Design and Thermophysical Property for Electronics and Energy 62 - 64 2010
• An extended dynamic model of a geyser induced by an inflow of gas (2): effects of various shapes and repeated expansions and contractions in an underground watercourse

  Kagami Hiroyuki

  Data Science Journal CODATA 9 IGY110 - IGY120 1683-1470 2010 [Refereed]
  
• An extended dynamical model of a geyser induced by inflow of gas: Considering effects of a complicated underground watercourse

  Hiroyuki Kagami

  Advances in Geosciences: Volume 17: Hydrological Science (HS) World Scientific Publishing Co. 17 103 - 111 2010/01 [Refereed]
  
• An extended dynamical model of a geyser induced by inflow of gas in case of plural underground gas supply sources

  Hiroyuki Kagami

  Advances in Geosciences: Volume 17: Hydrological Science (HS) World Scientific Publishing Co. 17 113 - 121 2010/01 [Refereed]
  
• Characteristic three-dimensional structure of resist's distribution after drying a resist solution coated on a flat substrate: analysis using the extended dynamical model of the drying process

  Hiroyuki Kagami

  ADVANCES IN RESIST MATERIALS AND PROCESSING TECHNOLOGY XXVI SPIE-INT SOC OPTICAL ENGINEERING 7273 0277-786X 2009 [Refereed]
  
• A modified dynamical model of drying process of polymer blend solution coated on a flat substrate

  Hiroyuki Kagami

  PHOTOMASK AND NEXT-GENERATION LITHOGRAPHY MASK TECHNOLOGY XV, PTS 1 AND 2 SPIE-INT SOC OPTICAL ENGINEERING 7028 0277-786X 2008 [Refereed]
  
• Nonlinear dynamics that appears in the dynamical model of drying process of a polymer solution coated on a flat substrate - art. no. 641704

  Hiroyuki Kagami

  Complexity and Nonlinear Dynamics SPIE-INT SOC OPTICAL ENGINEERING 6417 41704 - 41704 0277-786X 2007 [Refereed]
  
• Verification of the modified model of-drying process of a polymer liquid film on a flat substrate by experiment (3) - using organic solvent - art. no. 66071X

  Hiroyuki Kagami

  PHOTOMASK AND NEXT-GENERATION LITHOGRAPHY MASK TECHNOLOGY XIV, PTS 1 AND 2 SPIE-INT SOC OPTICAL ENGINEERING 6607 X6071 - X6071 0277-786X 2007 [Refereed]
  
• Verification of the modified model of drying process of a polymer liquid film on a flat substrate by experiment (2) - through more accurate experiment

  Hiroyuki Kagami

  PHOTOMASK AND NEXT GENERATION LITHOGRAPHY MASK TECHNOLOGY XIII, PTS 1 AND 2 SPIE-INT SOC OPTICAL ENGINEERING 6283 0277-786X 2006 [Refereed]
  

Books etc

• デジタル・AI時代の暮らし力 アナログ知のポテンシャル

  淺間正通; 山下巌; 小川あい; 木内明; 林順子; 佐川眞太郎; 志村昭暢; 梅宮悠; 酒井太一; 石井十郎; 関根正敏; 笹本浩; 前野博; 岩本勝幸; 鏡裕行 2020/05
• Fluid / Flow Analysis Casebook

  技術情報協会 (Joint workp.158～164)技術情報協会 2019/09 9784861047589 374p
• 粉体・微粒子分析 テクニック事例集

  椿淳一郎; 近藤郁; 鏡裕行ほか (Joint work)技術情報協会 2015
• ウェットプロセスによる精密薄膜コーティング技術

  真部高明; 薮上信; 鏡裕行ほか 技術情報協会 2014

Conference Activities & Talks

• Study of the eruption mechanism of Saturn's moon Enceladus plume using the mathematical model of a geyser (periodic bubbling spring)

  Hiroyuki Kagami

  12th IEEE Integrated STEM Education Conference  2022/03
• 山口県湯野温泉の活性化に向けた新・湯治の効能に関する調査

  鏡裕行; 寺田篤史; 中嶋克成; 羽田司

  第74回日本温泉科学会大会  2021/11
• The Modified Mathematical Model of Arteriosclerosis onset: Adding the Effect of Repairing Flaws of the Intima

  Hiroyuki Kagami

  The 12th International Symposium on ADVANCED TOPICS IN ELECTRICAL ENGINEERING  2021/03
• 立山カルデラ火口湖新湯の特徴的水位変化の2種の間欠泉数理モデルによる再現の試み

  鏡 裕行

  第73回日本温泉科学会大会  2020/11
• Fostering fundamental competences as healthcare professionals through study-support for children with a foreign citizenship

  Shinobu Hattori; Hiroyuki Kagami

  The 9th International Health Humanities Conference  2020/10
• 多発性嚢胞腎症モデルラットの腎臓における一次繊毛の形態変化

  熊本海生航; 齊藤成; 大野伸彦; 鏡裕行; 釘田雅則; 吉村文; 長尾静子

  第67回日本実験動物学会総会  2020/05
• The Modified Mathematical Model of the Pathogenesis of Urolithiasis: Add Calculi Dissolution Effect

  Hiroyuki Kagami

  International conference on Computer Applications & Information Security (ICCAIS’2020)  2020/03
• 改良された温泉沈殿物の成長の数理モデル：沈殿物の溶解の効果の付加

  鏡 裕行

  第72回日本温泉科学会大会  2019/11
• Estimation of the spouting time by the mathematical model that describes the periodic spouting of a geyser induced by boiling

  Hiroyuki Kagami

  The conference proceedings of the 22nd IEEE International Conference on Computational Science and Engineering (IEEE CSE 2019)  2019/08
• Evaluation of progression of the symptoms based on the mathematical model of arteriosclerosis onset and measures to control it

  Hiroyuki Kagami

  2019 IEEE SCLA - 1st Sustainable Cities Latin America Conference  2019/08
• 外国籍の児童・生徒への学習支援を通じた医療の専門職としての基礎力の育成（その3）

  鏡裕行; 服部しのぶ; 柿崎真沙子

  第51回日本医学教育学会大会  2019/07
• A mathematical model of arteriosclerosis onset

  Hiroyuki Kagami

  2nd Engineering Innovations in Healthcare International Conference  2019/03
• 立山カルデラの火口湖新湯の水位変動と水蒸気噴火との関係  [Not invited]

  福井幸太郎; 鏡裕行; 菊川茂; 飯田肇

  日本地理学会2019年春季学術大会  2019/03
• ヌクレオソームの凝集度の違いでクロマチンを分画する  [Not invited]

  石原悟; 笹川洋平; 琴村直恵; 山下 隼人; 鏡裕行; 阿部真之; 二階堂愛

  第41回日本分子生物学会年会  2018/11
• A mathematical model of spray drying granulation process in formulation

  Hiroyuki Kagami

  21st International Drying Symposium  2018/09
• 温泉沈殿物の成長の数理モデル  [Not invited]

  鏡裕行

  第71回日本温泉科学会大会  2018/09
• 外国籍の児童・生徒への学習支援を通じた医療の専門職としての基礎力の育成（その2）  [Not invited]

  鏡裕行; 塩竈和也; 服部しのぶ

  第50回日本医学教育学会大会  2018/08
• Suppression of Calculi Growth based on the Mathematical Model of the Pathogenesis of Urolithiasis

  Hiroyuki Kagami

  The International Conference on Advanced Communication Technologies and Networking (CommNet '18)  2018/04
• 塗布膜乾燥について  [Invited]

  鏡裕行

  日本粉体工業技術協会乾燥分科会 H29年度第2回分科会 講演会  2017/11
• 立山カルデラ内火口湖「新湯」の近年の不規則水位変動の機構の間欠沸騰泉の動力学モデルによる解析  [Not invited]

  鏡裕行; 福井幸太郎

  日本温泉科学会第70回大会  2017/09
• Reproduction of the transition between the spouting state and the pause state by the dynamical model of a geyser induced by gas inflow with consideration of influx of groundwater

  Hiroyuki Kagami

  The International Conference on Recent Trends in Environmental Science and Engineering  2017/08
• 外国籍の児童・生徒への学習支援を通じた医療の専門職としての基礎力の育成  [Not invited]

  鏡裕行; 塩竈和也; 服部しのぶ

  第49回日本医学教育学会大会  2017/08
• Further improvement of the unevenness of the edge portion of the coating film after drying by controlling the evaporation rate of the edge's side surface of the coating liquid film during drying

  Hiroyuki Kagami

  International Conference on Nanotechnology, Nanomaterials & Thin Films for Energy Applications  2017/07
• 立山カルデラ内火口湖「新湯」の近年の不規則水位変動の機構の数理モデルによる解析  [Not invited]

  鏡裕行; 福井幸太郎

  日本温泉科学会第69回大会  2016/09
• A model of drying process of a polymer liquid film coated on three-dimensional structure  [Not invited]

  Hiroyuki Kagami

  20th International Drying Symposium  2016/08
• Dependence of Locally Thicker Thin Film Formation by Partial Heating of a Polymer Solution Film on the Substrate from the Bottom during Drying on Latent Heat of Vaporization  [Not invited]

  Hiroyuki Kagami

  43rd International Symposium on Compound Semiconductors  2016/07
• The Influence of Partial Heating of a Polymer Solution Film Coated on Three-Dimensional Structure the Drying Process to the Thickness Profile of the Polymer Thin Film after Drying  [Not invited]

  Hiroyuki Kagami

  3rd International Conference on Nanotechnology, Nanomaterials & Thin Films for Energy Applications  2016/07
• 保育者養成校卒業後の動向調査その３－主に現職場の実態や退職理由に着目して―  [Not invited]

  原田明美; 吉見昌弘; 鏡裕行

  日本保育学会第69回大会  2016/05
• Mechanism of Locally Thicker Thin Film Formation by Partial Heating of a Polymer Solution Film on the Substrate from the Bottom during Drying  [Not invited]

  Hiroyuki Kagami

  The Ninth International Conference on Thermal Engineering Theory and Applications  2016/03
• Protection of geysers as tourism and environmental resources : Applications of the dynamical model of a geyser induced by gas inflow  [Not invited]

  Hiroyuki Kagami

  World Engineering Conference and Convention 2015  2015/12
• A static model of a geyser induced by gas inflow  [Not invited]

  Hiroyuki Kagami

  2nd International Conference on Mathematics and Computers in Sciences and Industry  2015/08
• A modified dynamical model of a geyser induced by gas inflow taking transition between spouting mode and pause mode and inflow of groundwater in the spouting pipe into consideration (2)  [Not invited]

  Hiroyuki Kagami

  The Asia Oceania Geosciences Society’s (AOGS) 12th Annual Meeting  2015/08

MISC

• The Mechanism of Drying of Coated Solution Film : Film Thickness Distribution after Drying of Various Types of Solutions Based on Numerical Simulation of the Mathematical Model

  Hiroyuki Kagami  塗装工学  56-  (9)  338  -349  2021/09  [Refereed]
  
• 立山カルデラ内火口湖「新湯」の近年の不規則水位変動の機構の間欠沸騰泉の動力学モデルによる解析

  鏡裕行; 福井幸太郎  日本温泉科学会大会講演要旨集  70th-  2017
• 立山カルデラ内火口湖「新湯」の近年の不規則水位変動の機構の数理モデルによる解析

  鏡裕行; 福井幸太郎  日本温泉科学会大会講演要旨集  69th-  2016
• Diffusion in solution in a nonequilibrium state such as vaporization process II

  Kagami Hiroyuki  Meeting abstracts of the Physical Society of Japan  58-  (2)  280  -280  2003/08
• Diffusion in solution in a nonequilibrium state such as vaporization process

  Kagami Hiroyuki  Meeting abstracts of the Physical Society of Japan  58-  (1)  329  -329  2003/03
• 27aPS-59 A model of drying process of liquid film including polymers coated on a flat surface

  Kagami H.; Miyagawa R.; Nakashima D.; Kobayashi S.; Kitano T.; Kawada A.; Takeshita K.; Kubota H.; Ohmi T.  Meeting abstracts of the Physical Society of Japan  57-  (1)  295  -295  2002/03
• 29p-P-6 Statistical estimation of vorticity distribution in a system described by anisotropic-dissipative Navier-Stokes eq.

  Kagami Hiroyuki  Meeting abstracts of the Physical Society of Japan  54-  (1)  771  -771  1999/03
• Statistical estimation of vorticity distribution in a system described by anisotropicdissipative Navier-Stokes eq.(III)

  KAGAMI Hiroyuki  Meeting abstracts of the Physical Society of Japan  53-  (2)  905  -905  1998/09
• Statistical estimation of vorticity distribution in a system described by anisotropic-dissipative Navier-Stokes eq.(II)

  KAGAMI Hiroyuki  Meeting abstracts of the Physical Society of Japan  53-  (1)  784  -784  1998/03
• 8a-YD-6 Statistical estimation of vorticity distribution in a system described by anisotropic-dissipative Navier-Stokes eq.

  Kagami Hiroyuki  Meeting abstracts of the Physical Society of Japan  52-  (2)  801  -801  1997/09

Research Grants & Projects

• 前向き研究に向けたIgA腎症の組織学的重症度分類の解析プラットフォームの構築

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2020/04 -2023/03 

  Author : 齊藤 成; 高橋 和男; 片山 鑑; 熊本 海生航; 鏡 裕行

   

  IgA腎症の糸球体と尿細管の解析プラットフォームの中心的役割であるプロテオミクス解析の質の向上に努めた。昨年度、糸球体40個をレーザーマイクロダイセクションで切り出して、LC-MSに解析し、約400個のタンパク質を解析できていた。本年度は、プロテオミクスの解析能力を増やすために、各ステップごとで改善点の洗い出しを行なった。その結果、糸球体40個をレーザーマイクロダイセクションで切り出して、L C-MSで解析し、約1100個以上タンパク質が同定できた。同定されたタンパク質の中には、糸球体特異的なタンパク質（ネフリン、ポドシンなど）も認められた。二倍以上の蛋白同定数の飛躍的な改善があった。改善を行うために、以下の点を検証し、解析方法を決定している。レーザーマイクロダイセクションで切り出す際の特殊な専用のスライドを３種類(POL,PEN,Director)によりプロテオミクスの解析に違いがあるのかを試した。超音波処理（Bioraptor, Covaris)は２種類の機器を試した。また、蛋白溶解液を２種類（PTS buffer, SDS buffer)による違いも試した。蛋白量の測定方法(microBCA, 2DQuant kit, EZQ kit)も検討した。蛋白抽出方法(PTS法、SP3法）である。同定数を大きく向上させたのは、蛋白抽出方法SP3法であった。SP3法は蛋白抽出液の中の界面活性剤の除去が優れていた。超音波処理のCovarisは蛋白抽出の能力は優れていたが、専用スライドのフィルムを掛けるとフィルムからのコンタミが起きてしまった。フィルム方式でないDirectorを用いて、SP3で試してみた結果、同定数は約５００個と同定数が悪かった。現在は、PEN or POL-PTS-Bioraptor-SP3の方法でIgA腎症の病態解析を進めている。

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