Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2014/04 -2017/03
Author : Tsuchiya Hiroyuki
We demonstrate in vivo fluorescence imaging of bone-marrow micrometastasis of prostate cancer at the cellular level in nude mice using PC-3 human prostate cancer cells labeled with green fluorescent protein (GFP). PC-3-GFP cells were visualized by real-time fluorescence imaging, to traffic and grow in the bone marrow. Formation of bone marrow micrometastasis could be imaged at the single-cell level in live mice, using confocal microscopy.
We investigated the efficacies of fluorescence-guided surgery (FGS), genetically-modified auxotrophic strain of Salmonella typhimurium (A1-R), and glycogen synthase kinase-3β (GSK- 3β) inhibitors. FGS significantly reduced residual tumor as well as the recurrence of experimental prostate-cancer bone metastasis. A1-R significantly inhibited tumor growth and metastasis in soft tissue tumor model and bone metastasis model. Treatment with GSK-3β inhibitors attenuated the growth of orthotopic osteosarcoma in mice.