木村　浩明 (キムラ　ヒロアキ)

研究者情報

J-Global ID

• 202001007562748289

研究キーワード

• 骨軟部腫瘍   転移性骨腫瘍   

研究分野

• ライフサイエンス / 整形外科学 / 骨軟部腫瘍

経歴

• 2020年10月 - 現在  名古屋市立大学大学院医学研究科 整形外科学分野講師
• 2016年04月 - 2020年10月  金沢赤十字病院整形外科第一整形外科部長
• 2014年04月 - 2016年03月  名古屋市立大学大学院医学研究科 整形外科学分野病院講師
• 2011年09月 - 2014年03月  金沢大学整形外科助教
• 2010年06月 - 2011年08月  金沢大学整形外科医員
• 2008年02月 - 2010年06月  カリフォルニア大学サンディエゴ校外科ポスドク

学歴

• 2002年04月 - 2011年03月   金沢大学   大学院医薬保健学総合研究科
• 1996年04月 - 2002年03月   金沢大学   医学部   医学科

研究活動情報

論文

• Long-term follow-up of chronic osteomyelitis after bone tumor resection.

  Hiroaki Kimura; Hisaki Aiba; Shiro Saito; Takao Sakai; Hideki Murakami

  Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 102597 - 102597 2024年12月
• Risk Factors and Impact on Survival of Pathological Fractures in Patients With Humeral Metastasis.

  Shiro Saito; Hiroaki Kimura; Hisaki Aiba; Yohei Kawaguchi; Hideki Murakami

  Cureus 16 11 e73382  2024年11月
• Recombinant Methioninase Synergistically Reverses High-docetaxel Resistance Developed in Osteosarcoma Cells.

  Sei Morinaga; Qinghong Han; Kohei Mizuta; Byung M Kang; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  Anticancer research 44 11 4773 - 4778 2024年11月
• Recombinant Methioninase Increases Eribulin Efficacy 16-fold in Highly Eribulin-resistant HT1080 Fibrosarcoma Cells, Demonstrating Potential to Overcome the Clinical Challenge of Drug-resistant Soft-tissue Sarcoma.

  Sei Morinaga; Qinghong Han; Kohei Mizuta; Byung Mo Kang; Motokazu Sato; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  Anticancer research 44 9 3777 - 3783 2024年09月
• Overcoming High Trabectedin Resistance of Soft-tissue Sarcoma With Recombinant Methioninase: A Potential Solution of a Recalcitrant Clinical Problem.

  Sei Morinaga; Qinghong Han; Kohei Mizuta; Byung M Kang; Motokazu Sato; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  Anticancer research 44 9 3785 - 3791 2024年09月
• Recombinant Methioninase Is Selectively Synergistic With Doxorubicin Against Wild-type Fibrosarcoma Cells Compared to Normal Cells and Overcomes Highly-Doxorubicin-resistant Fibrosarcoma.

  Sei Morinaga; Qinghong Han; Kohei Mizuta; Byung Mo Kang; Motokazu Sato; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  Anticancer research 44 8 3261 - 3268 2024年08月
• DNA-Binding Agent Trabectedin Combined With Recombinant Methioninase Is Synergistic to Decrease Fibrosarcoma Cell Viability and Induce Nuclear Fragmentation But Not Synergistic on Normal Fibroblasts.

  Sei Morinaga; Qinghong Han; Yutaro Kubota; Kohei Mizuta; Byung Mo Kang; Motokazu Sato; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  Anticancer research 44 6 2359 - 2367 2024年06月
• Extensive Synergy Between Recombinant Methioninase and Eribulin Against Fibrosarcoma Cells But Not Normal Fibroblasts.

  Sei Morinaga; Qinghong Han; Yutaro Kubota; Kohei Mizuta; Byung Mo Kang; Motokazu Sato; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Robert M Hoffman

  Anticancer research 44 3 921 - 928 2024年03月
• The Role of Microsurgery and Fluorescent-reporter Genes in Establishing Mouse Models for Real-Time Imaging of Metastatic Cancer-Cell Trafficking and Colony Formation: A Revolutionary and Disruptive Technology for Metastasis Research.

  Sei Morinaga; Norio Yamamoto; Kensuke Yamauchi; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  Cancer diagnosis & prognosis 4 5 544 - 557 2024年
• Extensive DNA Damage and Loss of Cell Viability Occur Synergistically With the Combination of Recombinant Methioninase and Paclitaxel on Pancreatic Cancer Cells which Report DNA-Damage Response in Real Time.

  Sei Morinaga; Qinghong Han; Kohei Mizuta; Byung Mo Kang; Motokazu Sato; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  Cancer genomics & proteomics 21 6 585 - 590 2024年
• Accurate and Safe Tumor Targeting of Orally-administered Salmonella typhimurium A1-R Leads to Regression of an Aggressive Fibrosarcoma in Nude Mice.

  Sei Morinaga; Ming Zhao; Kohei Mizuta; Byung Mo Kang; Motokazu Sato; Michael Bouvet; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya; Satoru Demura; Robert M Hoffman

  In vivo (Athens, Greece) 38 6 2601 - 2609 2024年
• Special Issue: "Pediatric Orthopedic Malignancy: Types, Symptoms, and Treatment".

  Hisaki Aiba; Shinji Miwa; Hideki Murakami; Hiroaki Kimura

  Children (Basel, Switzerland) 10 9 2023年09月
• Bone Metastases from Gastric Cancer Resembling Paget's Disease: A Case Report.

  Hisaki Aiba; Tomoharu Nakazato; Hideo Matsuo; Hiroaki Kimura; Shiro Saito; Takao Sakai; Hideki Murakami; Jun Kawai; Shingo Kawasaki; Yasuhiro Imamura

  Journal of clinical medicine 11 24 2022年12月 [査読有り]
  
• Protocol for a randomized study of the efficacy of ibandronic acid plus eldecalcitol in patients with gastric cancer after gastrectomy: A comparative study of different routes of administration of ibandronic acid

  Hisaki Aiba; Nobuyuki Suzuki; Hiroyuki Sagawa; Hiroya Hashimoto; Hiroaki Kimura; Jumpei Inoue; Takuya Usami; Yuta Goto; Yuichiro Mizuno; Tomotaka Okubo; Sunao Ito; Shuji Takiguchi; Hideki Murakami

  F1000Research 11 796 - 796 2022年07月
• Management of Soft-tissue Tumors With a Size of 2-5 cm, Including Malignancy.

  Yoshihiro Araki; Norio Yamamoto; Toru Maeda; Hiroaki Kimura; Takashi Ota; Shingo Shimozaki; Takashi Kato; Daisuke Inoue; Takashi Higuchi; Kensaku Abe; Yuta Taniguchi; Hiroyuki Tsuchiya

  Anticancer research 42 3 1555 - 1562 2022年03月
• Reverse lateral upper arm flaps for treating large soft tissue defects extending from the elbow to the forearm.

  Hideki Okamoto; Yohei Kawaguchi; Shinji Miwa; Hisaki Aiba; Hiroya Senda; Satona Murakami; Kazuo Hayakawa; Yuji Joyo; Hideki Murakami; Hiroaki Kimura

  Case reports in plastic surgery & hand surgery 9 1 145 - 150 2022年
• Review: Precise sarcoma patient-derived orthotopic xenograft (PDOX) mouse models enable identification of novel effective combination therapies with the cyclin-dependent kinase inhibitor palbociclib: A strategy for clinical application.

  Takashi Higuchi; Kentaro Igarashi; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Michael Bouvet; Hiroyuki Tsuchiya; Robert M Hoffman

  Frontiers in oncology 12 957844 - 957844 2022年
• Osteosarcoma Patient-derived Orthotopic Xenograft (PDOX) Models Used to Identify Novel and Effective Therapeutics: A Review.

  Takashi Higuchi; Kentaro Igarashi; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Michael Bouvet; Hiroyuki Tsuchiya; Robert M Hoffman

  Anticancer research 41 12 5865 - 5871 2021年12月
• Multifocal Epithelioid Hemangioendothelioma Complicated with Disseminated Intravascular Coagulation

  Hiroaki Kimura; Norio Yamamoto; Katsuhiro Hayashi; Takashi Higuchi; Hiroyuki Tsuchiya

  Case Reports in Oncology 14 3 1490 - 1496 2021年10月
• Effect of Neoadjuvant Therapies on Soft Tissue Sarcomas with Tail-like Lesions: A Multicenter Retrospective Study.

  Hisaki Aiba; Kunihiro Ikuta; Kunihiro Asanuma; Katsuhisa Kawanami; Satoshi Tsukushi; Akihiko Matsumine; Daisuke Ishimura; Akihito Nagano; Yoji Shido; Eiji Kozawa; Kenji Yamada; Junji Wasa; Hiroaki Kimura; Takao Sakai; Hideki Murakami; Tomohisa Sakai; Tomoki Nakamura; Yoshihiro Nishida

  Cancers 13 15 2021年08月
• Significant Improvement After Surgery for a Symptomatic Osteoblastoma in a Patient with Camurati-Engelmann Disease: Case Report and Literature Review.

  Hirotaka Yonezawa; Katsuhiro Hayashi; Norio Yamamoto; Akihiko Takeuchi; Kaoru Tada; Shinji Miwa; Kentaro Igarashi; Hiroaki Kimura; Yu Aoki; Sei Morinaga; Yoshihiro Araki; Yohei Asano; Keisuke Sakurakichi; Hiroko Ikeda; Takayuki Nojima; Hiroyuki Tsuchiya

  Calcified tissue international 108 6 819 - 824 2021年06月
• Myelolipoma mimicking osteosarcoma in the distal femur.

  Takao Sakai; Hisaki Aiba; Motoo Nakagawa; Hideo Hattori; Hideki Murakami; Hiroaki Kimura

  International journal of surgery case reports 83 105997 - 105997 2021年06月
• Combination methionine-methylation-axis blockade: A novel approach to target the methionine addiction of cancer

  Takashi Higuchi; Qinghong Han; Norihiko Sugisawa; Jun Yamamoto; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M. Hoffman

  Cancer Genomics and Proteomics 18 2 113 - 120 2021年04月
• Correction to: Treatment strategy for atypical ulnar fracture due to severely suppressed bone turnover caused by long-term bisphosphonate therapy: a case report and literature review.

  Kensaku Abe; Hiroaki Kimura; Norio Yamamoto; Shingo Shimozaki; Takashi Higuchi; Yuta Taniguchi; Takaaki Uto; Hiroyuki Tsuchiya

  BMC musculoskeletal disorders 22 1 9 - 9 2021年01月
• Multikinase-Inhibitor Screening in Drug-resistant Osteosarcoma Patient-derived Orthotopic Xenograft Mouse Models Identifies the Clinical Potential of Regorafenib

  TAKASHI HIGUCHI; KENTARO IGARASHI; NORIO YAMAMOTO; KATSUHIRO HAYASHI; HIROAKI KIMURA; SHINJI MIWA; MICHAEL BOUVET; HIROYUKI TSUCHIYA; ROBERT M. HOFFMAN

  Cancer Genomics - Proteomics 18 5 637 - 643 2021年
• Different patterns of pneumothorax in patients with soft tissue tumors treated with pazopanib: A case series analysis.

  Hisaki Aiba; Hiroaki Kimura; Satoshi Yamada; Hideki Okamoto; Katsuhiro Hayashi; Shinji Miwa; Yohei Kawaguchi; Shiro Saito; Takao Sakai; Tsutomu Tatematsu; Ryoichi Nakanishi; Hideki Murakami

  PloS one 16 7 e0254866  2021年
• Low-grade myofibroblastic sarcoma of the levator scapulae muscle: a case report and literature review.

  Hirotaka Yonezawa; Norio Yamamoto; Katsuhiro Hayashi; Akihiko Takeuchi; Shinji Miwa; Kentaro Igarashi; Mickhael Bang Langit; Hiroaki Kimura; Shingo Shimozaki; Takashi Kato; Sei Morinaga; Yoshihiro Araki; Yohei Asano; Hiroko Ikeda; Takayuki Nojima; Hiroyuki Tsuchiya

  BMC musculoskeletal disorders 21 1 836 - 836 2020年12月
• Treatment strategy for atypical ulnar fracture due to severely suppressed bone turnover caused by long-term bisphosphonate therapy: a case report and literature review.

  Kensaku Abe; Hiroaki Kimura; Norio Yamamoto; Shingo Shimozaki; Takashi Higuchi; Yuta Taniguchi; Takaaki Uto; Hiroyuki Tsuchiya

  BMC musculoskeletal disorders 21 1 802 - 802 2020年12月
• Osimertinib regressed an EGFR-mutant lung-adenocarcinoma bone-metastasis mouse model and increased long-term survival.

  Takashi Higuchi; Norihiko Sugisawa; Jun Ho Park; Yu Sun; Guangwei Zhu; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Translational oncology 13 10 100826 - 100826 2020年10月
• Efficacy of combination-chemotherapy with pirarubicin, ifosfamide, and etoposide for soft tissue sarcoma: a single-institution retrospective analysis.

  Shiro Saito; Hisaki Aiba; Satoshi Yamada; Hideki Okamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Takanobu Otsuka; Hideki Murakami

  BMC cancer 20 1 868 - 868 2020年09月
• 軟骨肉腫に対する非ステロイド性抗炎症薬によるPPARγを標的とした新しい治療戦略の可能性

  樋口 貴史; 山本 憲男; 林 克洋; 武内 章彦; 木村 浩明; 三輪 真嗣; 五十嵐 健太郎; 米澤 宏隆; 森永 整; 山本 靖彦; 土屋 弘行

  日本整形外科学会雑誌 94 8 S1981 - S1981 (公社)日本整形外科学会 2020年09月
• 軟部肉腫に対するolaratumabの可能性 patient-derived orthotopic xenograft(PDOX)マウスモデルによる解析

  樋口 貴史; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 三輪 真嗣; 五十嵐 健太郎; 米澤 宏隆; 森永 整; 荒木 麗博; 土屋 弘行

  日本整形外科学会雑誌 94 8 S1985 - S1985 (公社)日本整形外科学会 2020年09月
• Improvement of locomotive syndrome with surgical treatment in patients with degenerative diseases in the lumbar spine and lower extremities: a prospective cohort study.

  Satoshi Kato; Yuki Kurokawa; Tamon Kabata; Satoru Demura; Hidenori Matsubara; Yoshitomo Kajino; Yoshiyuki Okamoto; Hiroaki Kimura; Kazuya Shinmura; Kentaro Igarashi; Takaki Shimizu; Noritaka Yonezawa; Noriaki Yokogawa; Hiroyuki Tsuchiya

  BMC musculoskeletal disorders 21 1 515 - 515 2020年08月
• Recombinant Methioninase Combined With Tumor-targeting Salmonella typhimurium A1-R Induced Regression in a PDOX Mouse Model of Doxorubicin-resistant Dedifferentiated Liposarcoma.

  Kentaro Igarashi; Kei Kawaguchi; Ming Zhao; Qinghong Han; Yuying Tan; Tasuku Kiyuna; Kentaro Miyake; Takashi Higuchi; Scott D Nelson; Sarah M Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Anticancer research 40 5 2515 - 2523 2020年05月
• Combination of oral recombinant methioninase and decitabine arrests a chemotherapy-resistant undifferentiated soft-tissue sarcoma patient-derived orthotopic xenograft mouse model.

  Takashi Higuchi; Qinghong Han; Kentaro Miyake; Hiromichi Oshiro; Norihiko Sugisawa; Yuying Tan; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Biochemical and biophysical research communications 523 1 135 - 139 2020年02月
• The combination of oral-recombinant methioninase and azacitidine arrests a chemotherapy-resistant osteosarcoma patient-derived orthotopic xenograft mouse model.

  Takashi Higuchi; Norihiko Sugisawa; Jun Yamamoto; Hiromichi Oshiro; Qinghong Han; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Yuying Tan; Shreya Kuchipudi; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Cancer chemotherapy and pharmacology 85 2 285 - 291 2020年02月
• Exquisite Tumor Targeting by Salmonella A1-R in Combination with Caffeine and Valproic Acid Regresses an Adult Pleomorphic Rhabdomyosarcoma Patient-Derived Orthotopic Xenograft Mouse Model.

  Kentaro Igarashi; Kei Kawaguchi; Ming Zhao; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Scott D Nelson; Sarah M Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Takashi Higuchi; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Translational oncology 13 2 393 - 400 2020年02月
• Patient-derived orthotopic xenograft models of sarcoma.

  Kentaro Igarashi; Kei Kawaguchi; Takashi Murakami; Kentaro Miyake; Tasuku Kiyuna; Masuyo Miyake; Yukihiko Hiroshima; Takashi Higuchi; Hiromichi Oshiro; Scott D Nelson; Sarah M Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Cancer letters 469 332 - 339 2020年01月
• PPARγ Agonist Pioglitazone in Combination with Cisplatinum Arrests a Chemotherapy-resistant Osteosarcoma PDOX Model

  Takashi Higuchi; Jun Yamamoto; Norihiko Sugisawa; Yoshihiko Tashiro; Hiroto Nishino; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Shree R.A.M. Singh; Hiroyuki Tsuchiya; Robert M. Hoffman

  Cancer Genomics and Proteomics 17 1 35 - 40 2020年
• Pioglitazone, an agonist of PPARγ, reverses doxorubicin-resistance in an osteosarcoma patient-derived orthotopic xenograft model by downregulating P-glycoprotein expression.

  Takashi Higuchi; Norihiko Sugisawa; Kentaro Miyake; Hiromichi Oshiro; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Zoey Kline; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 118 109356 - 109356 2019年10月
• The Combination of Olaratumab with Doxorubicin and Cisplatinum Regresses a Chemotherapy-Resistant Osteosarcoma in a Patient-Derived Orthotopic Xenograft Mouse Model.

  Takashi Higuchi; Norihiko Sugisawa; Kentaro Miyake; Hiromichi Oshiro; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Translational oncology 12 9 1257 - 1263 2019年09月
• Oral Recombinant Methioninase, Combined With Oral Caffeine and Injected Cisplatinum, Overcome Cisplatinum-Resistance and Regresses Patient-derived Orthotopic Xenograft Model of Osteosarcoma.

  Takashi Higuchi; Hiromichi Oshiro; Kentaro Miyake; Norihiko Sugisawa; Qinghong Han; Yuying Tan; Junho Park; Zhiying Zhang; Sahar Razmjooei; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Sant P Chawla; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Anticancer research 39 9 4653 - 4657 2019年09月
• Combination Treatment With Sorafenib and Everolimus Regresses a Doxorubicin-resistant Osteosarcoma in a PDOX Mouse Model.

  Takashi Higuchi; Norihiko Sugisawa; Kentaro Miyake; Hiromichi Oshiro; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Zoey Kline; Paige Belt; Sant P Chawla; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Anticancer research 39 9 4781 - 4786 2019年09月
• Sorafenib and Palbociclib Combination Regresses a Cisplatinum-resistant Osteosarcoma in a PDOX Mouse Model.

  Takashi Higuchi; Norihiko Sugisawa; Kentaro Miyake; Hiromichi Oshiro; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Sant P Chawla; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Anticancer research 39 8 4079 - 4084 2019年08月
• Olaratumab combined with doxorubicin and ifosfamide overcomes individual doxorubicin and olaratumab resistance of an undifferentiated soft-tissue sarcoma in a PDOX mouse model.

  Takashi Higuchi; Kentaro Miyake; Norihiko Sugisawa; Hiromichi Oshiro; Zhiying Zhang; Sahar Razmjooei; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Cancer letters 451 122 - 127 2019年06月
• The combination of olaratumab with gemcitabine and docetaxel arrests a chemotherapy-resistant undifferentiated soft-tissue sarcoma in a patient-derived orthotopic xenograft mouse model.

  Takashi Higuchi; Kentaro Miyake; Norihiko Sugisawa; Hiromichi Oshiro; Zhiying Zhang; Sahar Razmjooei; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Cancer chemotherapy and pharmacology 83 6 1075 - 1082 2019年06月
• Pazopanib regresses a doxorubicin-resistant synovial sarcoma in a patient-derived orthotopic xenograft mouse model.

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Scott D Nelson; Tara A Russell; Sarah M Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Takashi Higuchi; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Tissue & cell 58 107 - 111 2019年06月
• Trabectedin and irinotecan combination regresses a cisplatinum-resistant osteosarcoma in a patient-derived orthotopic xenograft nude-mouse model.

  Takashi Higuchi; Kentaro Miyake; Hiromichi Oshiro; Norihiko Sugisawa; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Sant P Chawla; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Biochemical and biophysical research communications 513 2 326 - 331 2019年05月
• Treatment of a Malignant Soft Tissue Tumor Arising in the Vicinity of the Sciatic Nerve with an In-Situ Preparation Technique and Intensive Multidisciplinary Therapy.

  Hisaki Aiba; Katsuhiro Hayashi; Satoshi Yamada; Hideki Okamoto; Hiroaki Kimura; Shinji Miwa; Hiroyuki Inatani; Takanobu Otsuka; Hideki Murakami

  Cancers 11 4 2019年04月
• Osimertinib Regresses an EGFR-Mutant Cisplatinum- Resistant Lung Adenocarcinoma Growing in the Brain in Nude Mice.

  Takashi Higuchi; Hiromichi Oshiro; Zhiying Zhang; Kentaro Miyake; Norihiko Sugisawa; Yuki Katsuya; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Ming Zhao; Michael Bouvet; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Translational oncology 12 4 640 - 645 2019年04月
• The combination of gemcitabine and nab-paclitaxel as a novel effective treatment strategy for undifferentiated soft-tissue sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model.

  Takashi Higuchi; Kei Kawaguchi; Kentaro Miyake; Hiromichi Oshiro; Zhiying Zhang; Sahar Razmjooei; Sintawat Wangsiricharoen; Kentaro Igarashi; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Scott D Nelson; Sarah M Dry; Yunfeng Li; Sant P Chawla; Frederick C Eilber; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 111 835 - 840 2019年03月
• A prospective clinical trial to assess the accuracy of an MRI-based patient-specific acetabular instrument guide in total hip arthroplasty.

  Daisuke Inoue; Tamon Kabata; Hiroaki Kimura; Hiroyuki Tsuchiya

  European journal of orthopaedic surgery & traumatology : orthopedie traumatologie 29 1 65 - 71 2019年01月
• Metabolic targeting with recombinant methioninase combined with palbociclib regresses a doxorubicin-resistant dedifferentiated liposarcoma.

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyaki; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Takashi Higuchi; Arun S Singh; Bartosz Chmielowski; Scott D Nelson; Tara A Russell; Mark A Eckardt; Sarah M Dry; Yunfeng Li; Shree Ram Singh; Sant P Chawla; Fritz C Eilber; Hiroyuki Tsuchiya; Robert M Hoffman

  Biochemical and biophysical research communications 506 4 912 - 917 2018年12月
• Oral Recombinant Methioninase Combined with Caffeine and Doxorubicin Induced Regression of a Doxorubicin-resistant Synovial Sarcoma in a PDOX Mouse Model.

  Takashi Higuchi; Kei Kawaguchi; Kentaro Miyake; Qinghong Han; Yuying Tan; Hiromichi Oshiro; Norihiko Sugisawa; Zhiying Zhang; Sahar Razmjooei; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Sant P Chawla; Arun S Singh; Frederick C Eilber; Shree Ram Singh; Hiroyuki Tsuchiya; Robert M Hoffman

  Anticancer research 38 10 5639 - 5644 2018年10月
• Temozolomide regresses a doxorubicin-resistant undifferentiated spindle-cell sarcoma patient-derived orthotopic xenograft (PDOX): precision-oncology nude-mouse model matching the patient with effective therapy

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Yunfeng Li; Scott D. Nelson; Sarah M. Dry; Arun S. Singh; Irmina A. Elliott; Tara A. Russell; Mark A. Eckardt; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  Journal of Cellular Biochemistry 119 8 6598 - 6603 2018年08月 [査読有り]
  
• Growth of epiphysis after epiphyseal-preservation surgery for childhood osteosarcoma around the knee joint.

  Akihiko Takeuchi; Norio Yamamoto; Katsuhiro Hayashi; Hidenori Matsubara; Hiroaki Kimura; Shinji Miwa; Takashi Higuchi; Kensaku Abe; Yuta Taniguchi; Hiroyuki Tsuchiya

  BMC musculoskeletal disorders 19 1 185 - 185 2018年06月
• Treatment outcomes of the simple bone cyst

  Takashi Higuchi; Norio Yamamoto; Toshiharu Shirai; Katsuhiro Hayashi; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Kensaku Abe; Yuta Taniguchi; Hiroyuki Tsuchiya

  Medicine (United States) 97 18 e0572  2018年05月 [査読有り]
  
• Mid- to long-term clinical outcome of giant cell tumor of bone treated with calcium phosphate cement following thorough curettage and phenolization

  Akihiko Takeuchi; Prakrit Suwanpramote; Norio Yamamoto; Toshiharu Shirai; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Takashi Higuchi; Kensaku Abe; Hiroyuki Tsuchiya

  Journal of Surgical Oncology 117 6 1232 - 1238 2018年05月 [査読有り]
  
• Calcium phosphate cement in the surgical management of benign bone tumors

  Takashi Higuchi; Norio Yamamoto; Katsuhiro Hayashi; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Kensaku Abe; Yuta Taniguchi; Hisaki Aiba; Hiroyuki Tsuchiya

  Anticancer Research 38 5 3031 - 3035 2018年05月 [査読有り]
  
• Anti-tumor effects of a nonsteroidal anti-inflammatory drug zaltoprofen on chondrosarcoma via activating peroxisome proliferator-activated receptor gamma and suppressing matrix metalloproteinase-2 expression.

  Takashi Higuchi; Akihiko Takeuchi; Seiichi Munesue; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Inatani; Shingo Shimozaki; Takashi Kato; Yu Aoki; Kensaku Abe; Yuta Taniguchi; Hisaki Aiba; Hideki Murakami; Ai Harashima; Yasuhiko Yamamoto; Hiroyuki Tsuchiya

  Cancer medicine 7 5 1944 - 1954 2018年05月 [査読有り]
  
• Recombinant methioninase combined with doxorubicin (DOX) regresses a DOX-resistant synovial sarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model

  Kentaro Igarashi; Kei Kawaguchi; Shukuan Li; Qinghong Han; Yuying Tan; Emily Gainor; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Takashi Higuchi; Hiromichi Oshiro; Arun S. Singh; Mark A. Eckardt; Scott D. Nelson; Tara A. Russell; Sarah M. Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  Oncotarget 9 27 19263 - 19272 2018年04月 [査読有り]
  
• Preoperative evaluation of the efficacy of radio-hyperthermo-chemotherapy for soft tissue sarcoma in a case series

  Hisaki Aiba; Satoshi Yamada; Jun Mizutani; Norio Yamamoto; Hideki Okamoto; Katsuhiro Hayashi; Hiroaki Kimura; Akihiko Takeuchi; Shinji Miwa; Takashi Higuchi; Kensaku Abe; Yuta Taniguchi; Yoshihiro Araki; Hiroyuki Tsuchiya; Takanobu Otsuka

  PLoS ONE 13 4 e0195289  2018年04月 [査読有り]
  
• Clinical outcomes of radio-hyperthermo-chemotherapy for soft tissue sarcoma compared to a soft tissue sarcoma registry in Japan: a retrospective matched-pair cohort study

  Hisaki Aiba; Satoshi Yamada; Jun Mizutani; Norio Yamamoto; Hideki Okamoto; Katsuhiro Hayashi; Hiroaki Kimura; Akihiko Takeuchi; Shinji Miwa; Akira Kawai; Kenichi Yoshimura; Hiroyuki Tsuchiya; Takanobu Otsuka

  Cancer Medicine 7 4 1560 - 1571 2018年04月 [査読有り]
  
• Growth of doxorubicin-resistant undifferentiated spindle-cell sarcoma PDOX is arrested by metabolic targeting with recombinant methioninase

  Kentaro Igarashi; Shukuan Li; Qinghong Han; Yuying Tan; Kei Kawaguchi; Takashi Murakami; Tasuku Kiyuna; Kentaro Miyake; Yunfeng Li; Scott D. Nelson; Sarah M. Dry; Arun S. Singh; Irmina A. Elliott; Tara A. Russell; Mark A. Eckardt; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  Journal of Cellular Biochemistry 119 4 3537 - 3544 2018年04月 [査読有り]
  
• Recombinant methioninase in combination with doxorubicin (DOX) overcomes first-line DOX resistance in a patient-derived orthotopic xenograft nude-mouse model of undifferentiated spindle-cell sarcoma

  Kentaro Igarashi; Kei Kawaguchi; Shukuan Li; Qinghong Han; Yuying Tan; Takashi Murakami; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Arun S. Singh; Mark A. Eckardt; Scott D. Nelson; Tara A. Russell; Sarah M. Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Shree Ram Singh; Fritz C. Eilber; Robert M. Hoffman

  Cancer Letters 417 168 - 173 2018年03月 [査読有り]
  
• Tumor-targeting Salmonella typhimurium A1-R combined with recombinant methioninase and cisplatinum eradicates an osteosarcoma cisplatinum-resistant lung metastasis in a patient-derived orthotopic xenograft (PDOX) mouse model: decoy, trap and kill chemotherapy moves toward the clinic

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Shukuan Li; Qinghong Han; Yuying Tan; Ming Zhao; Yunfeng Li; Scott D. Nelson; Sarah M. Dry; Arun S. Singh; Irmina A. Elliott; Tara A. Russell; Mark A. Eckardt; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  Cell Cycle 17 6 801 - 809 2018年03月 [査読有り]
  
• Tumor-targeting Salmonella typhimurium A1-R is a highly effective general therapeutic for undifferentiated soft tissue sarcoma patient-derived orthotopic xenograft nude-mouse models

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Arun S. Singh; Mark A. Eckardt; Scott D. Nelson; Tara A. Russell; Sarah M. Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Shree Ram Singh; Fritz C. Eilber; Robert M. Hoffman

  Biochemical and Biophysical Research Communications 497 4 1055 - 1061 2018年03月 [査読有り]
  
• Temozolomide combined with irinotecan regresses a cisplatinumresistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) precision-oncology mouse model

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Yunfeng Li; Scott D. Nelson; Sarah M. Dry; Arun S. Singh; Irmina A. Elliott; Tara A. Russell; Mark A. Eckardt; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  Oncotarget 9 8 7774 - 7781 2018年 [査読有り]
  
• Knee joint preservation surgery in osteosarcoma using tumour-bearing bone treated with liquid nitrogen

  Takashi Higuchi; Norio Yamamoto; Hideji Nishida; Katsuhiro Hayashi; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Inatani; Shingo Shimozaki; Takashi Kato; Yu Aoki; Kensaku Abe; Yuta Taniguchi; Hiroyuki Tsuchiya

  INTERNATIONAL ORTHOPAEDICS 41 10 2189 - 2197 2017年10月 [査読有り]
  
• Temozolomide combined with irinotecan caused regression in an adult pleomorphic rhabdomyosarcoma patient-derived orthotopic xenograft (PDOX) nude-mouse model

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Takashi Murakami; Shinji Miwa; Scott D. Nelson; Sarah M. Dry; Yunfeng Li; Arun S. Singh; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  ONCOTARGET 8 44 75874 - 75880 2017年09月 [査読有り]
  
• A novel anionic-phosphate-platinum complex effectively targets an undifferentiated pleomorphic sarcoma better than cisplatinum and doxorubicin in a patient-derived orthotopic xenograft (PDOX)

  Kentaro Igarashi; Kei Kawaguchi; Takashi Murakami; Tasuku Kiyuna; Kentaro Miyake; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Scott D. Nelson; Sarah M. Dry; Yunfeng Li; Arun S. Singh; Shinji Miwa; Akira Odani; Fritz C. Eilber; Hiroyuki Tsuchiya; Robert M. Hoffman

  ONCOTARGET 8 38 63353 - 63359 2017年09月 [査読有り]
  
• High Efficacy of Pazopanib on an Undifferentiated Spindle-Cell Sarcoma Resistant to First-Line Therapy Is Identified With a Patient-Derived Orthotopic Xenograft (PDOX) Nude Mouse Model

  Kentaro Igarashi; Kei Kawaguchi; Takashi Murakami; Tasuku Kiyuna; Kentaro Miyake; Arun S. Singh; Scott D. Nelson; Sarah M. Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  JOURNAL OF CELLULAR BIOCHEMISTRY 118 9 2739 - 2743 2017年09月 [査読有り]
  
• Effective Metabolic Targeting of Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models with Recombinant Methioninase

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Takashi Murakami; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Robert M. Hoffman

  ANTICANCER RESEARCH 37 9 4807 - 4812 2017年09月 [査読有り]
  
• Efficacy In Vitro of Caffeine and Valproic Acid on Patient-Derived Undifferentiated Pleomorphic Sarcoma and Rhabdomyosarcoma Cell Lines

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Takashi Murakami; Shinji Miwa; Scott D. Nelson; Sarah M. Dry; Yunfeng Li; Arun S. Singh; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  ANTICANCER RESEARCH 37 8 4081 - 4084 2017年08月 [査読有り]
  
• Do Mesenchymal Stem Cells Derived From Atypical Lipomatous Tumors Have Greater Differentiation Potency Than Cells From Normal Adipose Tissues?

  Hiroyuki Inatani; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Akihiko Takeuchi; Shinji Miwa; Takashi Higuchi; Kensaku Abe; Yuta Taniguchi; Satoshi Yamada; Kiyofumi Asai; Takanobu Otsuka; Hiroyuki Tsuchiya

  CLINICAL ORTHOPAEDICS AND RELATED RESEARCH 475 6 1693 - 1701 2017年06月 [査読有り]
  
• An unusual case of proximal humeral simple bone cyst in an adult from secondary cystic change

  Mamer S. Rosario; Norio Yamamoto; Katsuhiro Hayashi; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Takashi Higuchi; Hiroyuki Inatani; Kensaku Abe; Yuta Taniguchi; Hisaki Aiba; Hiroyuki Tsuchiya

  WORLD JOURNAL OF SURGICAL ONCOLOGY 15 1 102  2017年05月 [査読有り]
  
• Antimetastatic Efficacy of the Combination of Caffeine and Valproic Acid on an Orthotopic Human Osteosarcoma Cell Line Model in Nude Mice

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Takashi Murakami; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Robert M. Hoffman

  ANTICANCER RESEARCH 37 3 1005 - 1011 2017年03月 [査読有り]
  
• Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model

  Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Takashi Murakami; Shinji Miwa; Scott D. Nelson; Sarah M. Dry; Yunfeng Li; Arun Singh; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  CELL CYCLE 16 1 91 - 94 2017年 [査読有り]
  
• Intra-arterial administration of tumor-targeting Salmonella typhimurium A1-R regresses a cisplatin-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model

  Kentaro Igarashi; Kei Kawaguchi; Takashi Murakami; Tasuku Kiyuna; Kentaro Miyake; Scott D. Nelson; Sarah M. Dry; Yunfeng Li; Jane Yanagawa; Tara A. Russell; Arun S. Singh; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C. Eilber; Robert M. Hoffman

  CELL CYCLE 16 12 1164 - 1170 2017年 [査読有り]
  
• Clinical Outcome of Reconstruction Using Frozen Autograft for a Humeral Bone Tumor

  Hiroaki Kimura; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Akihiko Takeuchi; Shinji Miwa; Hiroyuki Tsuchiya

  ANTICANCER RESEARCH 36 12 6631 - 6635 2016年12月 [査読有り]
  
• Surgical management of proximal fibular tumors: A report of 12 cases

  Hiroyuki Inatani; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Akihiko Takeuchi; Shinji Miwa; Takashi Higuchi; Kensaku Abe; Yuta Taniguchi; Satoshi Yamada; Hideki Okamoto; Takanobu Otsuka; Hiroyuki Tsuchiya

  JOURNAL OF BONE ONCOLOGY 5 4 163 - 166 2016年11月 [査読有り]
  
• Real-Time In Vivo Confocal Fluorescence Imaging of Prostate Cancer Bone-Marrow Micrometastasis Development at the Cellular Level in Nude Mice

  Shinji Miwa; Makoto Toneri; Kentaro Igarashi; Shuya Yano; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Hiroyuki Tsuchiya; Robert M. Hoffman

  JOURNAL OF CELLULAR BIOCHEMISTRY 117 11 2533 - 2537 2016年11月 [査読有り]
  
• Efficacy of glycogen synthase kinase-3β targeting against osteosarcoma via activation of ?-catenin.

  Shimozaki S; Yamamoto N; Domoto T; Nishida H; Hayashi K; Kimura H; Takeuchi A; Miwa S; Igarashi K; Kato T; Aoki Y; Higuchi T; Hirose M; Hoffman RM; Minamoto T; Tsuchiya H

  Oncotarget 7 47 77038 - 77051 2016年11月 [査読有り]
  
• Non-toxic Efficacy of the Combination of Caffeine and Valproic Acid on Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models

  Kentaro Igarashi; Norio Yamamoto; Katsuhiro Hayashi; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Robert M. Hoffman; Hiroyuki Tsuchiya

  ANTICANCER RESEARCH 36 9 4477 - 4482 2016年09月 [査読有り]
  
• The outcomes of reconstruction using frozen autograft combined with iodine-coated implants for malignant bone tumors: compared with non-coated implants

  Toshiharu Shirai; Hiroyuki Tsuchiya; Ryu Terauchi; Shinji Tsuchida; Naoki Mizoshiri; Kentaro Igarashi; Shinji Miwa; Akihiko Takeuchi; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Toshikazu Kubo

  JAPANESE JOURNAL OF CLINICAL ONCOLOGY 46 8 735 - 740 2016年08月 [査読有り]
  
• Prediction of skeletal-related events in patients with non-small cell lung cancer

  Hisaki Aiba; Tomoki Kimura; Takaya Yamagami; Nobuyuki Watanabe; Hironari Sakurai; Hiroaki Kimura; Shingo Shimozaki; Satoshi Yamada; Takanobu Otsuka

  Supportive Care in Cancer 24 8 3361 - 3367 2016年08月
• Treatment of a Simple Bone Cyst Using a Cannulated Hydroxyapatite Pin

  Toshiharu Shirai; Hiroyuki Tsuchiya; Ryu Terauchi; Shinji Tsuchida; Naoki Mizoshiri; Kazuya Ikoma; Hiroyoshi Fujiwara; Shinji Miwa; Hiroaki Kimura; Akihiko Takeuchi; Katsuhiro Hayashi; Norio Yamamoto; Toshikazu Kubo

  MEDICINE 94 25 e1027  2015年06月 [査読有り]
  
• Real-Time Fluorescence Imaging of the DNA Damage Repair Response During Mitosis

  Shinji Miwa; Shuya Yano; Mako Yamamoto; Yasunori Matsumoto; Fuminari Uehara; Yukihiko Hiroshima; Makoto Toneri; Takashi Murakami; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Elena V. Efimova; Hiroyuki Tsuchiya; Robert M. Hoffman

  JOURNAL OF CELLULAR BIOCHEMISTRY 116 4 661 - 666 2015年04月 [査読有り]
  
• Efficacy of triplet regimen antiemetic therapy for chemotherapy-induced nausea and vomiting (CINV) in bone and soft tissue sarcoma patients receiving highly emetogenic chemotherapy, and an efficacy comparison of single-shot palonosetron and consecutive-day granisetron for CINV in a randomized, single-blinded crossover study

  Hiroaki Kimura; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Akihiko Takeuchi; Kentaro Igarashi; Hiroyuki Inatani; Shingo Shimozaki; Takashi Kato; Yu Aoki; Takashi Higuchi; Hiroyuki Tsuchiya

  CANCER MEDICINE 4 3 333 - 341 2015年03月 [査読有り]
  
• Inhibition of spontaneous and experimental lung metastasis of soft-tissue sarcoma by tumor-targeting Salmonella typhimurium A1-R.

  Shinji Miwa; Yong Zhang; Kyung-Eun Baek; Fuminari Uehara; Shuya Yano; Mako Yamamoto; Yukihiko Hiroshima; Yasunori Matsumoto; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Michael Bouvet; Hiroyuki Tsuchiya; Robert M Hoffman; Ming Zhao

  Oncotarget 5 24 12849 - 61 2014年12月
• Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model.

  Shinji Miwa; Yukihiko Hiroshima; Shuya Yano; Yong Zhang; Yasunori Matsumoto; Fuminari Uehara; Mako Yamamoto; Hiroaki Kimura; Katsuhiro Hayashi; Michael Bouvet; Hiroyuki Tsuchiya; Robert M Hoffman

  Journal of orthopaedic research : official publication of the Orthopaedic Research Society 32 12 1596 - 601 2014年12月
• Fluorescence-guided surgery of prostate cancer bone metastasis.

  Shinji Miwa; Yasunori Matsumoto; Yukihiko Hiroshima; Shuya Yano; Fuminari Uehara; Mako Yamamoto; Yong Zhang; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Michael Bouvet; Naotoshi Sugimoto; Hiroyuki Tsuchiya; Robert M Hoffman

  The Journal of surgical research 192 1 124 - 33 2014年11月
• Outcomes and Complications of Reconstruction Using Tumor-bearing Frozen Autografts in Patients with Metastatic Bone Tumors

  Shinji Miwa; Akihiko Takeuchi; Toshiharu Shirai; Norio Yamamoto; Katsuhiro Hayashi; Hideji Nishida; Hiroaki Kimura; Kentaro Igarashi; Hiroyuki Tsuchiya

  ANTICANCER RESEARCH 34 10 5569 - 5577 2014年10月 [査読有り]
  
• Tumor-targeting Salmonella typhimurium A1-R prevents experimental human breast cancer bone metastasis in nude mice.

  Shinji Miwa; Shuya Yano; Yong Zhang; Yasunori Matsumoto; Fuminari Uehara; Mako Yamamoto; Yukihiko Hiroshima; Hiroaki Kimura; Katsuhiro Hayashi; Norio Yamamoto; Michael Bouvet; Hiroyuki Tsuchiya; Robert M Hoffman; Ming Zhao

  Oncotarget 5 16 7119 - 25 2014年08月
• Diagnosis and treatment of low-grade osteosarcoma: experience with nine cases

  Katsuhiro Hayashi; Hiroyuki Tsuchiya; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Inatani; Hideki Okamoto; Satoshi Yamada; Hiroko Ikeda; Seiko Sawada-Kitamura; Takayuki Nojima; Akishi Ooi; Takanobu Otsuka

  INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY 19 4 731 - 738 2014年08月 [査読有り]
  
• Synovial Sarcoma in Knee Joint, Mimicking Low-grade Sarcoma Confirmed by Molecular Detection of SYT Gene Split

  Hiroaki Kimura; Norio Yamamoto; Hider Nishida; Katsuhiro Hayashi; Akihiko Takeuchi; Takayuki Nojima; Hiroko Ikeda; Seiko Sawada-Kitamura; Hiroyuki Tsuchiya

  ANTICANCER RESEARCH 34 6 3105 - 3111 2014年06月 [査読有り]
  
• Late recurrence of osteosarcoma: a report of two cases

  Kentaro Igarashi; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Hiroaki Kimura; Akihiko Takeuchi; Shinji Miwa; Hiroyuki Inatani; Shingo Shimozaki; Takashi Kato; Hiroyuki Tsuchiya

  JOURNAL OF ORTHOPAEDIC SURGERY 22 3 415 - 419 2014年 [査読有り]
  
• Pedicle versus free frozen autograft for reconstruction in malignant bone and soft tissue tumors of the lower extremities

  Shingo Shimozaki; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Hiroaki Kimura; Akihiko Takeuchi; Kentaro Igarashi; Hiroyuki Inatani; Takashi Kato; Hiroyuki Tsuchiya

  JOURNAL OF ORTHOPAEDIC SCIENCE 19 1 156 - 163 2014年01月 [査読有り]
  
• Dynamic Color-Coded Fluorescence Imaging of the Cell-Cycle Phase, Mitosis, and Apoptosis Demonstrates How Caffeine Modulates Cisplatinum Efficacy

  Shinji Miwa; Shuya Yano; Yasunori Tome; Naotoshi Sugimoto; Yukihiko Hiroshima; Fuminari Uehara; Sumiyuki Mii; Hiroaki Kimura; Katsuhiro Hayashi; Elena V. Efimova; Toshiyoshi Fujiwara; Hiroyuki Tsuchiya; Robert M. Hoffman

  JOURNAL OF CELLULAR BIOCHEMISTRY 114 11 2454 - 2460 2013年11月 [査読有り]
  
• Huge and deeply situated glomus tumor: Its malignant form

  Toshiharu Shirai; Hiroyuki Tsuchiya; Norio Yamamoto; Katsuhiro Hayashi; Hideji Nishida; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Hiroko Ikeda

  Current Orthopaedic Practice 24 6 670 - 673 2013年11月 [査読有り]
  
• High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of α(v)β(3) integrin.

  Yasunori Tome; Hiroaki Kimura; Hiroki Maehara; Naotoshi Sugimoto; Michael Bouvet; Hiroyuki Tsuchiya; Fuminori Kanaya; Robert M Hoffman

  Anticancer research 33 9 3623 - 7 2013年09月
• Prognostic Value of Histological Response to Chemotherapy in Osteosarcoma Patients Receiving Tumor-Bearing Frozen Autograft

  Shinji Miwa; Akihiko Takeuchi; Hiroko Ikeda; Toshiharu Shirai; Norio Yamamoto; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Hiroaki Kimura; Kentaro Igarashi; Hiroyuki Tsuchiya

  PLOS ONE 8 8 e71362  2013年08月 [査読有り]
  
• Prognostic Value of Radiological Response to Chemotherapy in Patients with Osteosarcoma

  Shinji Miwa; Akihiko Takeuchi; Toshiharu Shirai; Junichi Taki; Norio Yamamoto; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Hiroaki Kimura; Kentaro Igarashi; Akishi Ooi; Hiroyuki Tsuchiya

  PLOS ONE 8 7 e70015  2013年07月 [査読有り]
  
• TNF-α and Tumor Lysate Promote the Maturation of Dendritic Cells for Immunotherapy for Advanced Malignant Bone and Soft Tissue Tumors

  Shinji Miwa; Hideji Nishida; Yoshikazu Tanzawa; Munetomo Takata; Akihiko Takeuchi; Norio Yamamoto; Toshiharu Shirai; Katsuhiro Hayashi; Hiroaki Kimura; Kentaro Igarashi; Eishiro Mizukoshi; Yasunari Nakamoto; Shuichi Kaneko; Hiroyuki Tsuchiya

  PLoS ONE 7 12 e52926 - e52926 2012年12月
• Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice.

  Kimura H; Tome Y; Momiyama M; Hayashi K; Tsuchiya H; Bouvet M; Hoffman RM

  International journal of cancer 131 9 2027 - 2033 2012年11月 [査読有り]
  
• Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways.

  Miwa S; Sugimoto N; Yamamoto N; Shirai T; Nishida H; Hayashi K; Kimura H; Takeuchi A; Igarashi K; Yachie A; Tsuchiya H

  Anticancer research 32 9 3643 - 3649 2012年09月 [査読有り]
  
• Treatment Strategies for Well-differentiated Liposarcomas and Therapeutic Outcomes

  Norio Yamamoto; Katsuhiro Hayashi; Yoshikazu Tanzawa; Hiroaki Kimura; Akihiko Takeuchi; Kentaro Igarashi; Hiroyuki Inatani; Shingo Shimozaki; Seiko Kitamura; Hiroyuki Tsuchiya

  ANTICANCER RESEARCH 32 5 1821 - 1825 2012年05月 [査読有り]
  
• Activity of bone morphogenetic protein-7 after treatment at various temperatures: Freezing vs. pasteurization vs. allograft

  Munetomo Takata; Naotoshi Sugimoto; Norio Yamamoto; Toshiharu Shirai; Katsuhiro Hayashi; Hideji Nishida; Yoshikazu Tanzawa; Hiroaki Kimura; Shinji Miwa; Akihiko Takeuchi; Hiroyuki Tsuchiya

  CRYOBIOLOGY 63 3 235 - 239 2011年12月 [査読有り]
  
• Functional outcomes after total scapulectomy for malignant bone or soft tissue tumors in the shoulder girdle

  Katsuhiro Hayashi; Mitsuaki Karita; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Akihiko Takeuchi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya

  INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY 16 5 568 - 573 2011年10月 [査読有り]
  
• UV Light Killing Efficacy of Fluorescent Protein-Expressing Cancer Cells In Vitro and In Vivo

  Hiroaki Kimura; Claudia Lee; Katsuhiro Hayashi; Kensuke Yamauchi; Norio Yamamoto; Hiroyuki Tsuchiya; Katsuro Tomita; Michael Bouvet; Robert M. Hoffman

  JOURNAL OF CELLULAR BIOCHEMISTRY 110 6 1439 - 1446 2010年08月 [査読有り]
  
• Real-Time Imaging of Single Cancer-Cell Dynamics of Lung Metastasis

  Hiroaki Kimura; Katsuhiro Hayashi; Kensuke Yamauchi; Norio Yamamoto; Hiroyuki Tsuchiya; Katsuro Tomita; Hiroyuki Kishimoto; Michael Bouvet; Robert M. Hoffman

  JOURNAL OF CELLULAR BIOCHEMISTRY 109 1 58 - 64 2010年01月 [査読有り]
  
• Caffeine-potentiated chemotherapy for metastatic osteosarcoma

  Hiroaki Kimura; Hiroyuki Tsuchiya; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Akihiko Takeuchi; Issei Ohnari; Katsuro Tomita

  JOURNAL OF ORTHOPAEDIC SCIENCE 14 5 556 - 565 2009年09月 [査読有り]
  
• Successful management of intractable infection after limb-saving surgery: A case report

  Hiroaki Kimura; Hiroyuki Tsuchiya; Toshiharu Shirai; Katsuhiro Hayashi; Hiroshi Hisakado; Akihiko Takeuchi; Katsuro Tomita

  Journal of Musculoskeletal Research 11 4 191 - 198 2008年 [査読有り]
  

MISC

• Patient-derived orthotopic xenograft models for osteosarcoma individualized precision treatment and effective drug discovery

  Takashi Higuchi; Kentaro Igarashi; Hiromichi Oshiro; Kentaro Miyake; Norihiko Sugisawa; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Zhenfeng Duan; Francis J. Hornicek; Hiroyuki Tsuchiya; Robert M. Hoffman Annals of Joint 6 2021年07月
• 軟部肉腫に対するolaratumabの可能性 patient-derived orthotopic xenograft(PDOX)マウスモデルによる解析

  樋口 貴史; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 三輪 真嗣; 五十嵐 健太郎; 米澤 宏隆; 森永 整; 荒木 麗博; 土屋 弘行 日本整形外科学会雑誌 94 (8) S1985 -S1985 2020年09月
• 脂肪由来幹細胞を用いた凍結自家処理骨移植後の再生促進作用の検討

  青木 裕; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 三輪 真嗣; 五十嵐 健太郎; 稲谷 弘幸; 谷口 裕太; 米澤 宏隆; 荒木 麗博; 森永 整; 杉本 直俊; 土屋 弘行 日本生体電気・物理刺激研究会誌 33 54 -54 2019年11月
• 脂肪由来幹細胞を用いた凍結自家処理骨移植後の再生促進作用の検討

  青木 裕; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 三輪 真嗣; 五十嵐 健太郎; 稲谷 弘幸; 谷口 裕太; 米澤 宏隆; 荒木 麗博; 森永 整; 杉本 直俊; 土屋 弘行 日本生体電気・物理刺激研究会誌 33 54 -54 2019年11月
• 脂肪由来幹細胞による凍結自家処理骨移植後の再生促進作用の検討

  青木 裕; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 三輪 真嗣; 五十嵐 健太郎; 稲谷 弘幸; 阿部 健作; 谷口 裕太; 荒木 麗博; 米澤 宏隆; 杉本 直俊; 土屋 弘行 日本整形外科学会雑誌 92 (6) S1457 -S1457 2018年06月
• 脂肪由来幹細胞による凍結自家処理骨移植後の再生促進作用の検討

  青木 裕; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 三輪 真嗣; 五十嵐 健太郎; 稲谷 弘幸; 阿部 健作; 谷口 裕太; 荒木 麗博; 米澤 宏隆; 杉本 直俊; 土屋 弘行 日本整形外科学会雑誌 92 (6) S1457 -S1457 2018年06月
• 脂肪由来幹細胞による自家凍結処理骨再生の検討

  青木 裕; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 稲谷 弘幸; 樋口 貴史; 阿部 健作; 谷口 裕太; 荒木 麗博; 土屋 弘行 日本整形外科学会雑誌 92 (3) S1106 -S1106 2018年03月
• 脂肪由来幹細胞による凍結処理骨再生の検討

  青木 裕; 山本 憲男; 林 克洋; 木村 浩明; 武内 章彦; 三輪 真嗣; 稲谷 弘幸; 樋口 貴史; 阿部 健作; 谷口 裕太; 相羽 久輝; 荒木 麗博; 土屋 弘行 日本整形外科学会雑誌 91 (8) S1644 -S1644 2017年08月
• Impact of excision repair cross-complementation group 1 (ERCCI) protein on survival of patients with osteosarcoma treated with cisplatin-based chemotherapy.

  Kentaro Igarashi; Norio Yamamoto; Toshiharu Shirai; Katsuhiro Hayashi; Hideji Nishida; Hiroaki Kimura; Akihiko Takeuchi; Shinji Miwa; Hiroyuki Inatani; Shingo Shimozaki; Takashi Kato; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Use of thallium scintigraphy and bone scintigraphy for differentiating between chondrosarcoma and chondroblastic osteosarcoma

  Takashi Kato; Norio Yamamoto; Hideji Nishida; Hiroaki Kimura; Akihiko Takeuchi; Shingo Shimozaki; Yu Aoki; Takashi Higuchi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Detection of MDM2 gene amplification in soft tissue sarcoma by FISH.

  Hiroaki Kimura; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Akihiko Takeuchi; Yoshikazu Tanzawa; Kentaro Igarashi; Shingo Shimozaki; Takashi Kato; Yu Aoki; Takashi Higuchi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Prognostic value of peroxisome proliferator-activated receptor gamma expression on clinical outcome of myxoid liposarcoma.

  Akihiko Takeuchi; Norio Yamamoto; Toshiharu Shirai; Katsuhiro Hayashi; Hideji Nishida; Yoshikazu Tanzawa; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Shingo Shimozaki; Takashi Kato; Yu Aoki; Takashi Higuchi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Minimally invasive surgery for simple bone cysts using hydroxyapatite cannulated pia: A long-term follow-up

  Takashi Higuchi; Norio Yamamoto; Hideji Nishida; Hiroaki Kimura; Akihiko Takeuchi; Shingo Shimozaki; Takashi Kato; Yu Aoki; Subhadrabandhu Saran; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Prognostic value of novel combined scoring system in patients with high-grade soft tissue sarcoma

  Akihiko Takeuchi; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Hiroaki Kimura; Shinji Miwa; Kentaro Igarashi; Shingo Shimozaki; Takashi Kato; Takashi Higuchi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Pelvic reconstruction for malignant bone tumors using recycled frozen autograft

  Yu Aoki; Norio Yamamoto; Toshiharu Shirai; Hideji Nishida; Katsuhiro Hayashi; Yoshikazu Tanzawa; Hiroaki Kimura; Akihiko Takeuchi; Kentaro Igarashi; Hiroyuki Inatani; Shingo Shimozaki; Takashi Kato; Takashi Higuchi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Long-term outcomes of tumor-bearing autografts treated with liquid nitrogen for reconstruction after tumor removal

  Norio Yamamoto; Kentaro Igarashi; Toshiharu Shirai; Katauhiro Hayashi; Hideji Nishida; Hiroaki Kimura; Akihiko Takeuchi; Shingo Shimozaki; Takeshi Kato; Yu Aoki; Takashi Higuchi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Dendritic cells immunotherapy for patients with malignant bone and soft tissue tumors.

  Hideji Nishida; Norio Yamamoto; Akihiko Takeuchi; Hiroaki Kimura; Shingo Shimozaki; Takashi Kato; Yu Aoki; Kentaro Igarashi; Takashi Higuchi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• Molecular targeted therapy for osteosarcoma using glycogen synthase kinase-3 beta (GSK-3 beta) inhibitors.

  Shingo Shimozaki; Norio Yamamoto; Hideji Nishida; Hiroaki Kimura; Akihiko Takeuchi; Takashi Kato; Yu Aoki; Takashi Higuchi; Toshinari Minamoto; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 32 (15) 2014年05月
• 骨肉腫におけるERCC1、MDR1、GSTP1の発現と予後

  五十嵐 健太郎; 山本 憲男; 白井 寿治; 西田 英司; 林 克洋; 丹沢 義一; 木村 浩明; 武内 章彦; 下崎 真吾; 加藤 貴士; 土屋 弘行 日本整形外科学会雑誌 87 (2) S280 -S280 2013年03月
• Marginal resection for osteosarcoma: Long-term outcomes

  Norio Yamamoto; Akihiko Takeuchi; Toshiharu Shirai; Katsuhiro Hayashi; Hiroaki Kimura; Kentaro Igarashi; Hiroyuki Tsuchiya JOURNAL OF CLINICAL ONCOLOGY 30 (15) 2012年05月
• Efficacy of novel platinum complexes against osteosarcoma bone targeting platinum and proteasome inhibitory platinum

  Kentaro Igarashi; Norio Yamamoto; Toshiharu Shirai; Katsuhiro Hayashi; Hideji Nishida; Akihiko Takeuchi; Yoshikazu Tanzawa; Hiroaki Kimura; Shinji Miwa; Hiroyuki Inatani; Shingo Shimozaki; Akira Odani; Hiroyuki Tsuchiya INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 30 S6 -S6 2012年
• Anti-beta 1 integrin antibody inhibits osteosarcoma lung seeding and metastasis

  Hiroaki Kimura; Yasunori Tome; John Grzesiak; Masashi Momiyama; Katsuhiro Hayashi; Michael Bouvet; Hiroyuki Tsuchiya; Robert M. Hoffman CANCER RESEARCH 71 2011年04月
• 肺におけるcancer cell dynamicsのリアルタイムイメージング(Real time imaging of cancer cell dynamics on the lung in the live mouse)

  山内 健輔; 木村 浩明; 林 克洋; 山本 憲男; 土屋 弘行; 富田 勝郎; Hoffman Robert M 日本癌学会総会記事 67回 181 -181 2008年09月
• 腫瘍標的サルモネラ株を使用した、マウス骨肉腫の原発巣及び肺転移の治療(Targeted therapy of bone tumor and lung metastasis in mice with a tumor-selective strain of Salmonella typhymurium)

  林 克洋; 土屋 弘行; 山本 憲男; 白井 寿治; 山内 健輔; 木村 浩明; 武内 章彦; ジョ・ミン; ブベイ・マイケル; ホフマン・ロバート M; 富田 勝郎 日本癌学会総会記事 67回 316 -317 2008年09月

共同研究・競争的資金等の研究課題

• 小児固形腫瘍治療に対する粒子線治療のための吸収性スペーサー留置ラットモデルの構築

  日本学術振興会：科学研究費助成事業

  研究期間 : 2021年04月 -2024年03月 

  代表者 : 亀井 美智; 岩田 宏満; 武田 理沙; 木村 浩明; 高木 大輔; 相羽 久輝

   

  本研究は、小児がん治療における陽子線照射時に安全に吸収性スペーサーを用いるため、ラットモデルを構築し、化学療法併用時の生体への影響について検証することを目的としている。 2021年度までに我々は、ラットモデルの構築のためのパイロット研究を終了し、研究方法を確立した。また既に本試験を開始している。 パイロット研究では、①ラットモデルに適切なスペーサーの大きさと手術手技の確立、②エンドキサン経口投与による化学療法のプロトコル確立、③安全性評価のための、血液毒性、炎症反応、病理組織による微小環境変化評価、CT撮像の画像解析の時期と項目の妥当性を検証した。成熟前のラットでは挿入可能なスペーサーの大きさが小さくなるため、吸収速度が速くなり評価が困難であった。さらにスペーサー留置部位、癒着防止シートの有無、大きさ、固定方法の検討を試みてプロトコルを確立した。実際には、成熟雄ラットを用い、留置術当日からエンドキサン投与を行い安全に解析が可能となっている。血液毒性については、エンドキサン投与の3－4日後に骨髄抑制が最も著明な傾向があり、28日以降は大きな変化が認められなかった。血液毒性、炎症反応の評価スケジュールを投与後3日後、1週間後、その後は1週間ごとに28日サイクルでの化学療法を実施することとした。Albの変化はラットモデルでは変化が認められず、中止とした。CT撮像については、撮像時期とともに、撮像スライス間隔、解像度の検討を行った。CT評価スケジュールは28日間隔とし、スペーサー断面の評価と減少率の解析が可能であった。 今後は、病理組織による微小環境の評価と合わせてスペーサー吸収形態の評価、二酸化炭素の産生、最終の吸収時期についても確認していく。
• 蛍光イメージングを用いた悪性骨軟部腫瘍の転移機序の解明と新規治療法の開発

  日本学術振興会：科学研究費助成事業

  研究期間 : 2014年04月 -2017年03月 

  代表者 : 土屋 弘行; 林 克洋; 山本 憲男; 三輪 真嗣; 木村 浩明

   

  前立腺癌細胞とヌードマウスを用いて骨転移初期変化の蛍光イメージングを行い、シングルセルレベルでの転移巣初期の変化として、腫瘍が骨に到達して転移巣を形成する過程を継時的に可視化することに成功した。 骨軟部肉腫における新規治療法として、蛍光ガイド下腫瘍切除術、腫瘍特異的バクテリア（A1-R）治療、GSK-3β阻害薬の有用性について検討した。蛍光ガイド下手術は前立腺癌の骨転移モデルにおいて腫瘍再発を有意に抑制した。A1-Rは軟部肉腫モデルと骨転移モデルのいずれにおいても腫瘍抑制効果を示した。GSK-3β阻害薬は骨肉腫モデルにおいて有意な腫瘍抑制効果を示した。
• 抗癌剤耐性や肺転移形成と細胞周期との関連―Fucci導入肉腫細胞を用いた解析

  日本学術振興会：科学研究費助成事業

  研究期間 : 2013年04月 -2016年03月 

  代表者 : 木村 浩明

   

  細胞周期をリアルタイムで観察できる蛍光蛋白”Fucci”を導入したヒト骨肉腫細胞株143Bにin vitroでシスプラチン・ドキソルビシンを作用させリアルタイムイメージングを行った。その結果、細胞周期による抗がん剤の感受性の違い、抗がん剤の種類によるアポトーシスの起こり方の違いや細胞周期停止の様子を明らかにできた。
• 骨肉腫の肺転移形成と血液凝固・線溶因子との関連

  日本学術振興会：科学研究費助成事業

  研究期間 : 2011年 -2012年 

  代表者 : 木村 浩明

   

  ヒト骨肉腫cell line "143B" の核にGFP、細胞質にRFPが発現した2色の蛍光を有する143B-dualを使用し、転移能の異なる複数のcell lineを最初に樹立した。それらの細胞におけるuPA、PAI-1、TFの発現量をElizaとPCRで確認し、uPAとPAI-1が転移能と強く関連している事が証明された。現在は143BにおけるuPAとPAI-1のshRNAを行い、in vivoにおいて転移能がどのように変化するかを観察する予定である。

その他のリンク

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