Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Date (from‐to) : 2018/04 -2021/03
Author : Sakamoto Nobuhiro
Peritoneal dissemination of gastric cancer is a serious disease with no effective treatment. In this study we examined effect of siRNA for anti-apoptotic survivin and Bcl2 antisense oligo (ASO) in MKN45 gastric cancer cells. SiRNA treatment for survivin did not work well and we designed and tested antisense sequence (AmNA type) for Bcl2 mRNA. Among many candidates we eventually chose 4 sequences after many validation steps using RT-PCR and western blotting in hepatoblastoma cells and HCT116 cells. ASO#2 displayed superior tumor inhibitory effect in combination with low dose cisplatin in MKN45 gastric cancer cells. The knockdown effect sustained for 72 h. This ASO may be a useful nucleic medicine for peritoneal dissemination of gastric cancer. To improve in vivo transduction efficiency, an EPR facilitating super carbonate apatite system may be useful.