細田　直ホソダ　ナオ

研究者基本情報

学歴

• 1997年 - 2002年, 東京大学大学院, 薬学系研究科, 機能薬学専攻
•  - 1997年, 東京大学, 薬学部, 薬学科

学位

• 東京大学薬学系研究科／博士（薬学）

経歴

•   2011年04月 - 現在, 名古屋市立大学大学院薬学研究科, 講師
•   2007年04月 - 2011年03月, 名古屋市立大学大学院薬学研究科, 助教
•   2006年06月 - 2007年03月, 名古屋市立大学大学院薬学研究科, 助手
•   2004年08月 - 2006年05月, 米国ロチェスター大学医学部歯学部, 博士研究員
•   2002年04月 - 2004年07月, 東京大学大学院医学系研究科, 学術研究支援員

研究活動情報

研究キーワード

ナンセンス変異, 遺伝子疾患, mRNA, 翻訳

論文

• TDP-43 accelerates deadenylation of target mRNAs by recruiting Caf1 deadenylase., Fukushima M, Hosoda N, Chifu K, Hoshino SI, FEBS letters, 593, (3) 277 - 287,   2018年12月, 査読有り
  DOI
• A novel method for stabilizing microRNA mimics., Nogimori T, Furutachi K, Ogami K, Hosoda N, Hoshino SI, Biochemical and biophysical research communications, 511, (2) 422 - 426,   2019年04月, 査読有り
  DOI
• Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway., Nogimori T, Nishiura K, Kawashima S, Nagai T, Oishi Y, Hosoda N, Imataka H, Kitamura Y, Kitade Y, Hoshino SI, Nucleic acids research, 47, (1) 432 - 449,   2018年11月, 査読有り
  DOI
• Proteolysis: a double-edged sword for the development of amyloidoses., Okamoto A, Hosoda N, Hoshino SI, Prion, 1 - 7,   2018年09月, 査読有り
  DOI
• Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail., Sawazaki R, Imai S, Yokogawa M, Hosoda N, Hoshino SI, Mio M, Mio K, Shimada I, Osawa M, Scientific reports, 8, (1) ,   2018年01月, 査読有り
  DOI
• Proteolysis suppresses spontaneous prion generation in yeast., Okamoto A, Hosoda N, Tanaka A, Newnam GP, Chernoff YO, Hoshino SI, The Journal of biological chemistry, 292, (49) 20113 - 20124,   2017年12月, 査読有り
  DOI
• Arsenite inhibits mRNA deadenylation through proteolytic degradation of Tob and Pan3., Yamagishi R, Hosoda N, Hoshino S, Biochemical and biophysical research communications, 455, (3-4) 323 - 331,   2014年12月, 査読有り
  DOI
• The processed isoform of the translation termination factor eRF3 localizes to the nucleus to interact with the ARF tumor suppressor., Hashimoto Y, Kumagai N, Hosoda N, Hoshino S, Biochemical and biophysical research communications, 445, (3) 639 - 644,   2014年03月, 査読有り
  DOI
• Antiproliferative protein Tob directly regulates c-myc proto-oncogene expression through cytoplasmic polyadenylation element-binding protein CPEB, K. Ogami, N. Hosoda, Y. Funakoshi, S. Hoshino, Oncogene, 33, 55 - 64,   2014年01月02日, The regulation of mRNA deadenylation constitutes a pivotal mechanism of the post-transcriptional control of gene expression. Here we show that the antiproliferative protein Tob, a component of the Caf1-Ccr4 deadenylase complex, is involved in regulating the expression of the proto-oncogene c-myc. The c-myc mRNA contains cis elements (CPEs) in its 3′-untranslated region (3′-UTR), which are recognized by the cytoplasmic polyadenylation element-binding protein (CPEB). CPEB recruits Caf1 deadenylase through interaction with Tob to form a ternary complex, CPEB-Tob-Caf1, and negatively regulates the expression of c-myc by accelerating the deadenylation and decay of its mRNA. In quiescent cells, c-myc mRNA is destabilized by the trans-acting complex (CPEB-Tob-Caf1), while in cells stimulated by the serum, both Tob and Caf1 are released from CPEB, and c-Myc expression is induced early after stimulation by the stabilization of its mRNA as an 'immediate-early gene'. Collectively, these results indicate that Tob is a key factor in the regulation of c-myc gene expression, which is essential for cell growth. Thus, Tob appears to function in the control of cell growth at least, in part, by regulating the expression of c-myc. © 2014 Macmillan Publishers Limited.
  https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891736073&origin=inward
  DOI
• The Hbs1-Dom34 protein complex functions in non-stop mRNA decay in mammalian cells., Saito S, Hosoda N, Hoshino S, The Journal of biological chemistry, 288, (24) 17832 - 17843,   2013年06月, 査読有り
  DOI
• Translation termination factor eRF3 is targeted for caspase-mediated proteolytic cleavage and degradation during DNA damage-induced apoptosis., Hashimoto Y, Hosoda N, Datta P, Alnemri ES, Hoshino S, Apoptosis : an international journal on programmed cell death, 17, (12) 1287 - 1299,   2012年12月, 査読有り
  DOI
• Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay., Osawa M, Hosoda N, Nakanishi T, Uchida N, Kimura T, Imai S, Machiyama A, Katada T, Hoshino S, Shimada I, RNA (New York, N.Y.), 18, (11) 1957 - 1967,   2012年11月, 査読有り
  DOI
• Anti-proliferative protein Tob negatively regulates CPEB3 target by recruiting Caf1 deadenylase., Hosoda N, Funakoshi Y, Hirasawa M, Yamagishi R, Asano Y, Miyagawa R, Ogami K, Tsujimoto M, Hoshino S, The EMBO journal, 30, (7) 1311 - 1323,   2011年04月, 査読有り
  DOI
• Quantitative characterization of Tob interactions provides the thermodynamic basis for translation termination-coupled deadenylase regulation., Ruan L, Osawa M, Hosoda N, Imai S, Machiyama A, Katada T, Hoshino S, Shimada I, The Journal of biological chemistry, 285, (36) 27624 - 27631,   2010年09月, 査読有り
  DOI
• Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay, Isken O, Kim YK, Hosoda N, Mayeur GL, Hershey JW, Maquat LE, Cell, 133, 314-327, (2) 314 - 327,   2008年04月, 査読有り
  DOI
• Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay, Sato H, Hosoda N, Maquat LE, Molecular cell, 29, 255-262, (2) 255 - 262,   2008年02月, 査読有り
  DOI
• Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases, Funakoshi Y, Doi Y, Hosoda N, Uchida N, Osawa M, Shimada I, Tsujimoto M, Suzuki T, Katada T, Hoshino S, Genes & development, 21, 3135-3148, (23) 3135 - 3148,   2007年12月, 査読有り
  DOI
• Failsafe nonsense-mediated decay does not detectably target eIF4E-bound mRNA., Matsuda D, Hosoda N, Kim YK, Maquat LE, Nature structural & molecular biology, 14, 974-979, (10) 974 - 979,   2007年10月, 査読有り
  DOI
• [Features of NMD (nonsense-mediated mRNA decay) in mammalian cells]., Hosoda N, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 51, (16 Suppl) 2544 - 2548,   2006年12月, 査読有り
• Evidence that poly(A) binding protein C1 binds nuclear pre-mRNA poly(A) tails., Hosoda N, Lejeune F, Maquat LE, Molecular and cellular biology, 26, (8) 3085 - 3097,   2006年04月, 査読有り
  DOI
• CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells., Hosoda N, Kim YK, Lejeune F, Maquat LE, Nature structural & molecular biology, 12/,893-901, (10) 893 - 901,   2005年10月, 査読有り
  DOI
• Translation termination factor eRF3 mediates mRNA decay through the regulation of deadenylation., Hosoda N, Kobayashi T, Uchida N, Funakoshi Y, Kikuchi Y, Hoshino S, Katada T, The Journal of biological chemistry, 278/,38287-38291, (40) 38287 - 38291,   2003年10月, 査読有り
  DOI
• Enzymaic and signal transduction properties of CD38/NADase and PC-1/　phosphodiesterase., Chem. Immunol., 75/,60-78,   2000年
• Novel function of the Eukaryotic Polypeptide-chain Releasing Factor 3 (eRF3/GSPT) in the Mechanism of mRNA Degradation., Biochemistry, 64/,1367-1372,   1999年
• Inhibition of phosphodiesterase/pyrophosphatase activity of PC-1 by its association with glycosaminoglycans., 265/,763-770,   1999年
  DOI
• Protein-tyrosine phosphorylation by IgG1 subclass CD38 monoclonal antibodies is mediated through stimulation of the FcgII receptors in human myeloid cell lines., J. Immunol., 159/,5226-5232,   1997年

MISC

• 哺乳動物細胞におけるNMDの分子機構とその特徴 (RNAと生命) -- (RNA動態), 細田 直, 蛋白質核酸酵素, 51, (16) 2544 - 2548,   2006年12月
  http://ci.nii.ac.jp/naid/40015203275
• 躍進するRNA研究 進展する構造解析,機能性RNAの多彩な役割の解明とRNAiなど生命・医工学への応用 第2章 RNAによる遺伝子発現調節 3)翻訳調節とmRNA動態 2.ウイルスRNA翻訳制御, 細田直, 野本明男, 実験医学, 22, (17) 2469 - 2474,   2004年11月25日
  http://jglobal.jst.go.jp/public/200902295471871690

特許

• 人工合成mRNAの発現を効率化する方法, 星野 真一, 細田 直, 野木森 拓人, 特願2016-219227, 特開2018-074954
• 人工合成mRNAの安定化方法, 星野 真一, 細田 直, 野木森 拓人, 特願2014-263857, 特開2015-226531
• 人工合成mRNAの翻訳効率化方法, 星野 真一, 細田 直, 野木森 拓人, 特願2014-107562, 特開2015-221026