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HAN YOUYUN

    Lecturer
Last Updated :2025/08/22

Researcher Information

Research funding number

  • 50566297

J-Global ID

Research Areas

  • Life sciences / Molecular biology

Academic & Professional Experience

  • Tokyo Institute of Technology School of Life Science and TechnologyResearcher
  • Kyoto University

Published Papers

MISC

Research Grants & Projects

  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2023/04 -2027/03 
    Author : 吉川 学; 韓 龍雲; 岩川 弘宙; 向原 隆文
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2018/04 -2021/03 
    Author : Yoshikawa Manabu
     
    RNA-induced silencing complexes (RISCs) containing a siRNA and an ARGONAUTE protein play an important role in development, stress responses and virus defense. In plants, RISCs induce secondary siRNA production from their targets. In this research we investigated the molecular mechanisms of secondary siRNA production through in vitro analyses using plant extracts. Our data showed that SGS3 and SDE5 cooperatively recruit RDR6 that function in dsRNA formation.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2016/04 -2019/03 
    Author : HAN YONG-WOON
     
    The Zero Mode Waveguides (ZMWs) enables us to visualize a single fluorescent molecule under high concentration of the molecules and characterize the protein-protein or protein-DNA complex formation process. I have already established the method for construction of ZMWs and characterized some of DNA-binding proteins using the ZMWs. However, research technique using ZMWs was not still common technique. For availability of ZMWs, in this study, I developed the method for construction of ZMWs using Character Projection electron beam lithography. The cost of ZMWs decreased to 1/5 and the production efficiency was increased to 10-fold compared with my previous construction method for ZMWs.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2013/04 -2016/03 
    Author : HAN YONG-WOON; IWASA Takuma
     
    Zero Mode Waveguides (ZMWs) consist of nanosized holes in an aluminum film. Thus, ZMWs enable us the visualization of a single fluorescent molecule under micromolar order of the molecules. We characterized E. coli RuvB motor protein, which promotes Holliday junction DNA branch migration, and SRA, hemi-methyl CpG binding domain. We observed fluorescently labeled RuvBs binding to a RuvA-Holliday junction under various nucleotide conditions and our data suggested that not only ATP binding to RuvB but also ATP hydrolysis by RuvB facilitates a stable RuvA-RuvB-Holliday junction DNA complex formation
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2011 -2012 
    Author : HAN Yong-woon; IWASA Takuma
     
    Single molecule imaging techniques enable us to measure and regulate movements of proteins or DNA. Using these techniques, I characterized E. coli UvrD helicase and RuvB motor protein, which promote branch migration of Holliday junction DNA. I also measured association and dissociation rates of Pyrrole-Imidazole polyamides and calculated their model structures to clarify the relationship between the DNA binding kinetics and Pyrrole-Imidazole polyamide structures.

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