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HAN YOUYUN

    Lecturer
Last Updated :2025/10/03

Researcher Information

Research funding number

  • 50566297

J-Global ID

Research Areas

  • Life sciences / Molecular biology

Academic & Professional Experience

  • Tokyo Institute of Technology School of Life Science and TechnologyResearcher
  • Kyoto University

Published Papers

MISC

Research Grants & Projects

  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2023/04 -2027/03 
    Author : 吉川 学; 韓 龍雲; 岩川 弘宙; 向原 隆文
     
    これまでの植物のRNAサイレンシングに関する研究によって、詳細な分子機構やウイルスに対する抵抗性における機能などが明らかにされてきた。近年、ウイルス以外にも、細菌やカビ、卵菌など広範な植物病原微生物に対する防御機構の一つとしてRNAサイレンシングが機能することが示唆されている。一方、それらの植物病原微生物やウイルスは、宿主のRNAサイレンシングを阻害するサプレッサーと呼ばれるタンパク質を有している。我々は、試験管内で植物のRNAサイレンシングを解析できる実験系を構築し、その分子機構を解析してきた。本研究ではこの実験系を用いて、植物病原微生物に由来するサプレッサーによるRNAサイレンシング抑制機構を明らかにすることを目的とする。 まず本研究では、様々な植物病原体に由来する既存サプレッサーによるRNAサイレンシング阻害の作用機作を試験管内実験系で解析し、分子レベルでの解明する。また植物病害細菌のエフェクターから宿主RNAサイレンシングを抑制するタンパク質を探索する。そのためにエフェクター遺伝子ライブラリーを準備して試験管内転写反応によりmRNAを作製した。次に試験管内翻訳反応でそれらのエフェクタータンパク質を調製した後、試験管内RNAサイレンシング実験系で阻害効果を調べる。阻害効果が確認できたエフェクターについては、阻害するステップの解析や質量分析などにより相互作用する宿主タンパク質を同定するなどの解析を行う。以上の研究を行い、様々な作物に植物病原微生物抵抗性の付与に繋がる知見を得ることを目指す。
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2023/04 -2027/03 
    Author : 吉川 学; 韓 龍雲; 岩川 弘宙; 向原 隆文
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2018/04 -2021/03 
    Author : Yoshikawa Manabu
     
    RNA-induced silencing complexes (RISCs) containing a siRNA and an ARGONAUTE protein play an important role in development, stress responses and virus defense. In plants, RISCs induce secondary siRNA production from their targets. In this research we investigated the molecular mechanisms of secondary siRNA production through in vitro analyses using plant extracts. Our data showed that SGS3 and SDE5 cooperatively recruit RDR6 that function in dsRNA formation.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2017/06 -2019/03 
    Author : Harada Yoshie; Tadakuma Hisashi; Nakao Kimiko; Han Yong-Woon; Fukumoto Kodai; Masubuchi Takeya
     
    Transcription is the first step of gene expression. To visualize the real-time process of transcription, we established the method using Zero-mode waveguides (ZMWs). We optimized the fabrication process and glass surface modification, which allowed us to minimize the fluctuation of ZMW-chip quality. In addition, it is important to control the precise three-dimensional (3D) layout of transcription factors, e.g. RNA polymerase (RNAP), as the 3D layout on the flexible DNA affects the interaction of RNAP and regulatory factors. Therefore, using DNA origami technology, we established new approach to control the molecular layout at nano-meter resolution. These methods should contribute to future elucidation of transcription mechanism.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2016/04 -2019/03 
    Author : HAN YONG-WOON
     
    The Zero Mode Waveguides (ZMWs) enables us to visualize a single fluorescent molecule under high concentration of the molecules and characterize the protein-protein or protein-DNA complex formation process. I have already established the method for construction of ZMWs and characterized some of DNA-binding proteins using the ZMWs. However, research technique using ZMWs was not still common technique. For availability of ZMWs, in this study, I developed the method for construction of ZMWs using Character Projection electron beam lithography. The cost of ZMWs decreased to 1/5 and the production efficiency was increased to 10-fold compared with my previous construction method for ZMWs.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2013/04 -2016/03 
    Author : HAN YONG-WOON; IWASA Takuma
     
    Zero Mode Waveguides (ZMWs) consist of nanosized holes in an aluminum film. Thus, ZMWs enable us the visualization of a single fluorescent molecule under micromolar order of the molecules. We characterized E. coli RuvB motor protein, which promotes Holliday junction DNA branch migration, and SRA, hemi-methyl CpG binding domain. We observed fluorescently labeled RuvBs binding to a RuvA-Holliday junction under various nucleotide conditions and our data suggested that not only ATP binding to RuvB but also ATP hydrolysis by RuvB facilitates a stable RuvA-RuvB-Holliday junction DNA complex formation
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2011 -2012 
    Author : HAN Yong-woon; IWASA Takuma
     
    Single molecule imaging techniques enable us to measure and regulate movements of proteins or DNA. Using these techniques, I characterized E. coli UvrD helicase and RuvB motor protein, which promote branch migration of Holliday junction DNA. I also measured association and dissociation rates of Pyrrole-Imidazole polyamides and calculated their model structures to clarify the relationship between the DNA binding kinetics and Pyrrole-Imidazole polyamide structures.

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