Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Date (from‐to) : 2019/04 -2022/03
Author : Matsunaga Tamihide
We used gellan gum (GG), a soluble dietary fiber, to optimize human iPS cell-derived enterocytes (hiPSEs) differentiation. hiPSEs cocultured with GG had significantly higher expression of small intestine- and pharmacokinetics-related genes and proteins. GG could improve the differentiation efficiency of human iPS cells to enterocytes and increase intestinal maturation by extending the life span of hiPSEs. Human iPS cell-derived budding-like organoids showed a high expression of drug transporters and induced the expression of CYP3A4 and P-glycoprotein. Upon dissociation and seeding on cell culture inserts, the organoids retained intestinal characteristics, forming polarized intestinal folds with approximately 400 Ω×cm2 transepithelial electrical resistance. We generated small intestine-on-a-chip and construct with human iPS cell-derived 2D organoids. These novel methods have great potential for disease modeling and drug screening applications.