MIYAJIMA Chiharu

Researcher Information

J-Global ID

• 201901019478884085

Research Areas

• Life sciences / Cell biology

Published Papers

• The Hippo Signaling Pathway Manipulates Cellular Senescence

  Chiharu Miyajima; Mai Nagasaka; Hiromasa Aoki; Kohki Toriuchi; Shogo Yamanaka; Sakura Hashiguchi; Daisuke Morishita; Mineyoshi Aoyama; Hidetoshi Hayashi; Yasumichi Inoue

  Cells 2024/12 [Refereed]
  
• ID3 is a novel target gene of p53 and modulates lung cancer cell metastasis

  Mai Nagasaka; Chiharu Miyajima; Yasumichi Inoue; Sakura Hashiguchi; Yuya Suzuki; Daisuke Morishita; Hiromasa Aoki; Kohki Toriuchi; Ryohei Katayama; Mineyoshi Aoyama; Hidetoshi Hayashi

  Biochemical and Biophysical Research Communications Elsevier BV 149789 - 149789 0006-291X 2024/03
• SET8 is a novel negative regulator of TGF-β signaling in a methylation-independent manner

  Mai Nagasaka; Yasumichi Inoue; Yuji Nagao; Chiharu Miyajima; Daisuke Morishita; Hiromasa Aoki; Mineyoshi Aoyama; Takeshi Imamura; Hidetoshi Hayashi

  Scientific Reports Springer Science and Business Media LLC 13 (1) 2023/12
• Involvement of cardiac glycosides targeting Na/K-ATPase in their inhibitory effects on c-Myc expression via its transcription, translation, and proteasomal degradation.

  Muneshige Tokugawa; Yasumichi Inoue; Hiromasa Aoki; Chiharu Miyajima; Kan'ichiro Ishiuchi; Kento Tsurumi; Chisane Kujirai; Daisuke Morishita; Michiyo Matsuno; Hajime Mizukami; Masaki Ri; Shinsuke Iida; Toshiaki Makino; Mineyoshi Aoyama; Hidetoshi Hayashi

  Journal of biochemistry 2023/11
• Insights into Regulators of p53 Acetylation

  Mai Nagasaka; Chiharu Miyajima; Hiromasa Aoki; Mineyoshi Aoyama; Daisuke Morishita; Yasumichi Inoue; Hidetoshi Hayashi

  Cells MDPI AG 11 (23) 3825 - 3825 2022/11
• 強心配糖体periplocinは多発性骨髄腫において小胞体ストレス応答を抑制し、抗がん活性を示す

  鯨井 千実; 徳川 宗茂; 井上 靖道; 石内 勘一郎; 松野 倫代; 李 政樹; 宮嶋 ちはる; 飯田 真介; 水上 元; 牧野 利明; 林 秀敏

  日本生化学会大会プログラム・講演要旨集 (公社)日本生化学会 95回 3T17m - 02 2022/11
• HMG-CoA Reductase Inhibitor Statins Activate the Transcriptional Activity of p53 by Regulating the Expression of TAZ.

  Chiharu Miyajima; Yurika Hayakawa; Yasumichi Inoue; Mai Nagasaka; Hidetoshi Hayashi

  Pharmaceuticals (Basel, Switzerland) 15 (8) 2022/08
• The deubiquitinating enzyme USP17 regulates c-Myc levels and controls cell proliferation and glycolysis.

  Mai Nagasaka; Yasumichi Inoue; Manaka Yoshida; Chiharu Miyajima; Daisuke Morishita; Muneshige Tokugawa; Haruna Nakamoto; Mayumi Sugano; Nobumichi Ohoka; Hidetoshi Hayashi

  FEBS letters 596 (4) 465 - 478 2022/02
• Periplocin and cardiac glycosides suppress the unfolded protein response.

  Muneshige Tokugawa; Yasumichi Inoue; Kan'ichiro Ishiuchi; Chisane Kujirai; Michiyo Matsuno; Masaki Ri; Yuka Itoh; Chiharu Miyajima; Daisuke Morishita; Nobumichi Ohoka; Shinsuke Iida; Hajime Mizukami; Toshiaki Makino; Hidetoshi Hayashi

  Scientific reports 11 (1) 9528 - 9528 2021/05
• Anti-Inflammatory Activity of Kurarinone Involves Induction of HO-1 via the KEAP1/Nrf2 Pathway.

  Sakiko Nishikawa; Yasumichi Inoue; Yuka Hori; Chiharu Miyajima; Daisuke Morishita; Nobumichi Ohoka; Shigeaki Hida; Toshiaki Makino; Hidetoshi Hayashi

  Antioxidants (Basel, Switzerland) 9 (9) 2020/09
• Transcriptional Coactivator TAZ Negatively Regulates Tumor Suppressor p53 Activity and Cellular Senescence.

  Chiharu Miyajima; Yuki Kawarada; Yasumichi Inoue; Chiaki Suzuki; Kana Mitamura; Daisuke Morishita; Nobumichi Ohoka; Takeshi Imamura; Hidetoshi Hayashi

  Cells 9 (1) 2020/01
• The ubiquitin-specific protease USP17 prevents cellular senescence by stabilizing the methyltransferase SET8 and transcriptionally repressing p21.

  Keishi Fukuura; Yasumichi Inoue; Chiharu Miyajima; Shin Watanabe; Muneshige Tokugawa; Daisuke Morishita; Nobumichi Ohoka; Masayuki Komada; Hidetoshi Hayashi

  The Journal of biological chemistry 294 (44) 16429 - 16439 2019/11
• Kurarinone from Sophora Flavescens Roots Triggers ATF4 Activation and Cytostatic Effects Through PERK Phosphorylation.

  Sakiko Nishikawa; Yuka Itoh; Muneshige Tokugawa; Yasumichi Inoue; Ken-Ichi Nakashima; Yuka Hori; Chiharu Miyajima; Kou Yoshida; Daisuke Morishita; Nobumichi Ohoka; Makoto Inoue; Hajime Mizukami; Toshiaki Makino; Hidetoshi Hayashi

  Molecules (Basel, Switzerland) 24 (17) 2019/08
• Elevated Plasma Levels of LDL Cholesterol Promote Dissecting Thoracic Aortic Aneurysms in Angiotensin II-Induced Mice.

  Hiroki Tanaka; Yasunori Iida; Takayuki Iwaki; Yuko Suzuki; Hideto Sano; Chiharu Miyajima; Nobuhiro Zaima; Takeshi Sasaki; Ayato Sumioka; Shogo Hakamata; Hideyuki Shimizu; Kazuo Umemura; Tetsumei Urano

  Annals of vascular surgery 48 204 - 213 2018/04
• Characterization of Atherosclerosis Formation in a Murine Model of Type IIa Human Familial Hypercholesterolemia.

  Chiharu Miyajima; Takayuki Iwaki; Kazuo Umemura; Victoria A Ploplis; Francis J Castellino

  BioMed research international 2018 1878964 - 1878964 2018
• Tribbles-Related Protein Family Members as Regulators or Substrates of the Ubiquitin-Proteasome System in Cancer Development.

  Satoshi Sakai; Chiharu Miyajima; Chiharu Uchida; Yuka Itoh; Hidetoshi Hayashi; Yasumichi Inoue

  Current cancer drug targets 16 (2) 147 - 56 2016
• Pseudokinase tribbles 1 (TRB1) negatively regulates tumor-suppressor activity of p53 through p53 deacetylation.

  Chiharu Miyajima; Yasumichi Inoue; Hidetoshi Hayashi

  Biological & pharmaceutical bulletin 38 (4) 618 - 24 2015
• Positive Regulation of Interleukin-2 Expression by a Pseudokinase, Tribbles 1, in Activated T Cells.

  Chiharu Miyajima; Yuka Itoh; Yasumichi Inoue; Hidetoshi Hayashi

  Biological & pharmaceutical bulletin 38 (8) 1126 - 33 2015

Research Grants & Projects

• 上皮間葉転換の生体内動態可視化によるがん転移の時空間的理解と新規治療法開発

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2021/04 -2024/03 

  Author : 井上 靖道; 林 秀敏; 宮嶋 ちはる; 今村 健志

   

  上皮間葉転換 (EMT) は、がんの浸潤・転移をはじめとしたがんの悪性化に深く関与している。しかしながら、EMT研究においては、in vitroで得られた結果と、in vivoでの研究結果との間で整合性がつかないことも多く、両者を隔てる原因として、生体における腫瘍の時間空間的多様性が考えられる。本研究では、EMTを起こしたがん細胞の生体内動態を可視化できるイメージングシステムを構築して、EMT形質の獲得および腫瘍化に重要な因子やシグナル伝達を明らかにし、がん転移を予防または克服する薬の創造的開発につながることを目的とした。 今年度の研究で以下の結果を得た。 （1）ヒトビメンチン（Vim）プロモーター、マウスVimプロモーターを元にしたEMT可視化レポータープラスミドを用いて、NMuMG及びA549をはじめとした各細胞株に導入しTGF-β、TNF-αによるEMT誘導とレポーター活性化を確認した。ビメンチン以外にも、間葉系マーカーN-cadherinとテネイシンC、上皮系マーカーE-cadherinを利用した可視化プローブの検討も実施した。（２）作成したEMT可視化を用いて、がん原遺伝子c-MycによるSnail誘導を介したEMT誘導機構の分子メカニズムを解析した。加えて、脱ユビキチン化酵素USP17がc-Mycの脱ユビキチン化を介してc-Mycタンパクを安定化させ、c-Mycによる細胞増殖とワールブルグ効果様代謝変化を促すことを明らかにし論文発表した。
• The Role of Blood Fibrinolytic Factor Plasmin Activity in Manipulating Macrophage-mediated Mechanisms of Atherosclerosis Promotion

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2018/04 -2022/03 

  Author : Miyajima Chiharu

   

  In this study, we analyzed the effect of plasminogen (Plg) on the development of atherosclerosis in mice double-deficient (Ldlr-/-/Apobec1-/-) in the LDL receptor and RNA-editing enzyme APOBEC1, a mouse model of human type IIa familial hyperlipidemia, the most common form of hyperlipidemia in humans. Plg did not affect the expression of scavenger receptors involved in LDL uptake by Mφ, suggesting a novel pathogenetic mechanism that controls Mφ atherosclerosis progression by directly regulating LDL size.

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https://researchmap.jp/cmcm